Description: Quantitative

Description: The Spike S1 (B.1.1.7 Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling inhibitors of the interaction of ACE2 with the B.1.1.7 Variant of the SARS-CoV-2 Spike S1 protein. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike S1 B.1.1.7 protein is coated on a 96-well transparent plate. Next, ACE2-Biotin is incubated with Spike S1 variant on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a luminometer or microplate reader capable of reding chemiluminescence.

Description: The Spike S1 RBD-B.1.351 (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling inhibitors of the interaction of ACE2 with the RBD region of the B.1.351 variant of the SARS-CoV-2 Spike S1 protein.

Description: The Spike RBD (SARS-CoV-2) UK variant (N501Y):ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling inhibitors of the interaction of ACE2 with the UK variant (N501Y) of the SARS-CoV-2 Spike RBD protein. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike RBD protein UK variant is coated on a 96-well transparent plate. Next, ACE2-Biotin is incubated with Spike RBD UK variant on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a luminometer or microplate reader capable of reading chemiluminescence.

Description: The Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling neutralizing antibodies or inhibitors of the interaction between the Omicron variant SARS-CoV-2 Spike RBD and human ACE2. This kit comes in a convenient 96-well format, with Biotinylated-ACE2, purified Spike RBD (Omicron Variant; B.1.1.529 BA.1) protein, Streptavidin-HRP, and assay buffers for 100 reactions. The assay requires only a few steps. First, SARS-CoV-2 Spike RBD (Omicron Variant; B.1.1.529 BA.1) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a chemiluminescence HRP substrate to produce the luminescence signal.

Description: The Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between the SARS-CoV-2 Omicron variant Spike Trimer and human ACE2. The SARS-CoV-2 Spike Trimer, included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/host cell interaction.The assay requires only a few steps. First, SARS-CoV-2 Spike Trimer (B.1.1.529 BA.1, Omicron Variant) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of chemiluminescence HRP substrate to produce the luminescence signal.

Description: The FTO Chemiluminescent Assay Kit is designed to measure FTO activity for screening and profiling applications. The key to the FTO Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to the activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FTO enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The G9a Direct Activity Chemiluminescent Assay Kit is designed to measure G9a_x000D_activity for screening and profiling applications. The key to the G9a Direct Activity Assay_x000D_Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With_x000D_this kit, only three simple steps are required for methyltransferase detection. First,_x000D_S-adenosylmethionine is incubated with a sample containing assay buffer_x000D_and methyltransferase enzyme for one hour. Next, primary antibody is added._x000D_Finally, the strips are treated with an HRP-labeled secondary antibody followed by_x000D_addition of the HRP substrate to produce chemiluminescence that can then be measured_x000D_using a chemiluminescence reader.

Description: The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity for screening and profiling applications. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The NSD2 Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The EZH1 Chemiluminescent Assay Kit is designed to measure activity of the EZH1 complex (EZH1/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH1 Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The NSD2 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD2_x000D_activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay_x000D_Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this_x000D_kit, only three simple steps are required for methyltransferase detection. First, S-_x000D_adenosylmethionine is incubated with a sample containing assay buffer and_x000D_methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated_x000D_with an HRP-labeled secondary antibody followed by addition of the HRP substrate to_x000D_produce chemiluminescence that can then be measured using a chemiluminescence_x000D_reader.

Description: The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity_x000D_for screening and profiling applications. The NSD3 Chemiluminescent Assay Kit comes in a_x000D_convenient format, with 96-well plate precoated with a specific substrate, the antibody against_x000D_methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine,_x000D_methyltransferase assay buffer, and purified NSD3 enzyme for 96 enzyme_x000D_reactions. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody_x000D_that recognizes methylated residue of Histone H3. With this kit, only three simple steps are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally,_x000D_the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP_x000D_substrate to produce chemiluminescence that can then be measured using a chemiluminescence_x000D_reader.

Description: The TET1 Chemiluminescent Assay Kit is designed to measure TET1 activity for screening and profiling applications. TET1 belongs to the Ten Eleven Translocation (TET) family proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET1 may repress gene transcription in a catalytic activity-independent manner_x000D__x000D_ The key to the TET1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes hydroxymethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of TET1 activity. First, the TET1 enzyme is incubated with the methylated substrate for two hours. Then the primary antibody is added. Next, the plate is treated with an HRP-labeled secondary antibody. Finally, the HRP substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. _x000D_

Description: The TET2 Chemiluminescent Assay Kit is designed to measure TET2 activity for screening and profiling applications. TET2 belongs to the Ten Eleven Translocation (TET) family proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET2 is one of the most frequently mutated genes in myeloid malignancies. _x000D__x000D__x000D__x000D_ The key to the TET2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes hydroxymethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of TET2 activity. First, TET2 enzyme is incubated with the methylated substrate for two hours. Then the primary antibody is added. Next, the plate is treated with an HRP-labeled secondary antibody. Finally, the HRP substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The EZH2 Direct Activity Chemiluminescent Assay Kit is designed to measure activity of the_x000D_EZH2 complex (EZH2/EED/SUZ12/RbAp48/AEBP) for screening and profiling_x000D_applications. The_x000D_key to the EZH2 Direct Activity Assay Kit is a highly specific antibody that recognizes_x000D_methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a_x000D_sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary_x000D_antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody_x000D_followed by addition of the HRP substrate to produce chemiluminescence that can then be_x000D_measured using a chemiluminescence reader._x000D_

Description: The EZH1 Chemiluminescent Assay Kit is designed to measure activity of the_x000D_EZH1 complex (EZH1/EED/SUZ12/RbAp48/AEBP) for screening and profiling purposes. The key to the EZH1 Chemiluminescent Assay Kit is_x000D_a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three_x000D_simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine_x000D_is incubated with a sample containing assay buffer and methyltransferase_x000D_enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled_x000D_secondary antibody followed by addition of the HRP substrate to produce_x000D_chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The EZH2 WT (Wild Type) Chemiluminescent Assay Kit is designed to measure activity of the EZH2 complex (EZH2/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.

Description: The GCN5 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of GCN5. The GCN5 Chemiluminescent Assay Kit is based on the GCN5 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader. _x000D_

Description: The p300 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of p300 or to measure histone acetyltransferase (HAT) activity for screening and profiling applications. Histone acetyltransferase p300 (also known as KAT3B) is involved in various cellular events, and its dysfunction is linked to a number of human diseases including Parkinson's, Huntington's, and Alzheimer's diseases. The P300 Chemiluminescent Assay Kit is based on the p300 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader._x000D_

Description: Cyclic GMP (cGMP) is an important regulatory molecule in the GPCR signaling cascade. Our cGMP ELISA Kits provide a highly sensitive method to measure low levels of cyclic GMP in a variety of sample types.

Description: Cyclic GMP (cGMP) is an important regulatory molecule in the GPCR signaling cascade. Our cGMP ELISA Kits provide a highly sensitive method to measure low levels of cyclic GMP in a variety of sample types.

Description: The SMYD4 Chemiluminescent Assay Kit is designed to measure SMYD4_x000D_activity for screening and profiling applications. The SMYD4 Chemiluminescent Assay Kit comes_x000D_in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against_x000D_methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine,_x000D_methyltransferase assay buffer, and purified SMYD4 enzyme for 96 enzyme_x000D_reactions. The key to the SMYD4 Chemiluminescent Activity Assay Kit is a highly specific_x000D_antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps_x000D_are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a_x000D_sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added._x000D_Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the_x000D_HRP substrate to produce chemiluminescence that can then be measured using a_x000D_chemiluminescence reader.

Description: The PRMT5 Direct Activity Chemiluminescent Assay Kit is designed to measure PRMT5_x000D_ activity for screening and profiling applications. The key to the PRMT5_x000D_ Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3_x000D_ residue of Histone H4. With this kit, only three simple steps on a microtiter plate are_x000D_ required for methyltransferase detection. First, S-adenosylmethionine is incubated with a_x000D_ sample containing assay buffer and methyltransferase enzyme for one hour. Next,_x000D_ primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary_x000D_ antibody followed by addition of the HRP substrate to produce chemiluminescence that_x000D_ can then be measured using a chemiluminescence reader.

Description: The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1_x000D_Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3_x000D_residue of Histone H4. With this kit, only three simple steps are required for_x000D_methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme. Next, primary antibody is added._x000D_Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by_x000D_addition of the HRP substrate to produce chemiluminescence that can then be measured_x000D_using a chemiluminescence reader.

Description: The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3_x000D_activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highly_x000D_specific antibody that recognizes methylated substrate. With this kit, only three simple_x000D_steps on a microtiter plate are required for methyltransferase detection. First,_x000D_Sadenosylmethionine is incubated with a sample containing assay buffer and_x000D_methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the_x000D_plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP_x000D_substrate to produce chemiluminescence that can then be measured using a_x000D_chemiluminescence reader.

Description: The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4_x000D_Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated_x000D_PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required_x000D_for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme. Next, primary antibody is added._x000D_Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by_x000D_addition of an HRP substrate to produce chemiluminescence that can then be measured_x000D_using a chemiluminescence reader.

Description: The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The SETD2 Direct Activity Chemiluminescent Assay Kit is designed to measure SETD2_x000D_activity for screening and profiling applications. The key to the SETD2_x000D_Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36_x000D_residue of Histone H3. With this kit, only three simple steps on a microtiter plate are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a_x000D_sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary_x000D_antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody_x000D_followed by addition of the HRP substrate to produce chemiluminescence that can then be_x000D_measured using a chemiluminescence reader.

Description: The PRMT8 Chemiluminescent Assay Kit is designed to measure PRMT8_x000D_activity for screening and profiling applications. The key to the PRMT8 Chemiluminescent Assay Kit is a highly specific_x000D_antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple_x000D_steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine_x000D_is incubated with a sample containing assay buffer and methyltransferase_x000D_enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled_x000D_secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that_x000D_can then be measured using a chemiluminescence reader.

Description: The PRMT8 Chemiluminescent Assay Kit is designed to measure PRMT8 activity for screening and profiling applications. The PRMT8 Chemiluminescent Assay Kit comes in a convenient format with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine 3 (R3) residue of Histone H4, the secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified PRMT8 enzyme for 100 enzyme reactions. The key to the PRMT8 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine isincubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PRMT5 Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PRDM9 Chemiluminescent Assay Kit is designed to measure PRDM9 activity for screening and profiling applications, using purified PRDM9. The PRDM9 Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and enough purified PRDM9 enzyme for 100 enzyme reactions. The key to the PRDM9 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The PARP7 Chemiluminescent Activity Assay kit is designed to measure PARP7 activity for screening and profiling applications. The key to the PARP7 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP7 Chemiluminescent Activity Assay Kit is designed to measure PARP7 activity for screening and profiling applications. PARP7 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 384-well plate. Next, the PARP7 biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Description: The PARP12 Chemiluminescent Assay Kit is designed to measure PARP12 activity for screening and profiling applications. PARP12 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the PARP12 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP12 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP12 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The EHMT1 (GLP) Direct Activity Chemiluminescent Assay Kit is designed to measure EHMT1, also known as G9a-like protein or GLP activity for screening and profiling applications. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primaryantibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1_x000D_activity for screening and profiling applications. The key to the SetDB1 Direct Activity Assay Kit is a highly specific antibody that_x000D_recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally,_x000D_the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP_x000D_substrate to produce chemiluminescence that can then be measured using a chemiluminescence_x000D_reader.

Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The DNMT3A Direct Activity Assay Kit is designed to measure DNMT3A activity for screening and profiling applications. The key to the DNMT3A Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3A activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3A/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The DNMT3B Direct Activity Assay Kit is designed to measure DNMT3B activity for screening and profiling applications. The key to the DNMT3B Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3B activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3B/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The METTL3 Chemiluminescent Assay Kit is designed to measure METTL3 activity for screening and profiling applications. The METTL3 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated adenosine, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified METTL3 enzyme for 96 enzyme reactions. The key to the METTL3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes N6methylated adenosine. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The ALKBH5 Chemiluminescent Assay Kit is designed to measure ALKBH5 activity for screening and profiling applications. The key to the ALKBH5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to ALKBH5 demethylase activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, ALKBH5 enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Description: The PARP10 Chemiluminescent Assay Kit is designed to measure PARP10 activity for screening and profiling applications. The key to the PARP10 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP10 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP10 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader

Description: The PARP11 Chemiluminescent Activity Assay kit is designed to measure PARP11 activity for screening and profiling applications. The key to the PARP11 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP11 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP11 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Description: The EZH2 (A738T) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (A738T)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.

Description: The EZH2 (Y641F) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.

Description: The EZH2 (Y641N) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes.