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Quantitative T cell repertoire analysis by deep cDNA sequencing of T cell receptor α and β chains using next-generation sequencing (NGS).

Immune responses play a essential function in numerous illness situations together with most cancers and autoimmune ailments. Nonetheless, so far, there has not been a speedy, delicate, complete, and quantitative evaluation technique to look at T-cell or B-cell immune responses. Right here, we report a brand new strategy to characterize T cell receptor (TCR) repertoire by sequencing hundreds of thousands of cDNA of TCR α and β chains together with a newly-developed algorithm.
Utilizing samples from lung most cancers sufferers handled with most cancers peptide vaccines as a mannequin, we show that detailed data of the V-(D)-J mixture together with complementary figuring out area 3 (CDR3) sequences will be decided. We recognized in depth irregular splicing of TCR transcripts in lung most cancers samples, indicating the dysfunctional splicing equipment in T lymphocytes by prior chemotherapy.
As well as, we discovered three probably novel TCR exons that haven’t been described beforehand within the reference genome. This newly developed TCR NGS platform will be utilized to raised perceive immune responses in lots of illness areas together with immune issues, allergy symptoms, and organ transplantations.
NGS catalog: A database of subsequent technology sequencing research in people.
Subsequent technology sequencing (NGS) applied sciences have been quickly utilized in biomedical and organic analysis since its introduction just a few years in the past, and they’re anticipated to advance at an unprecedented tempo within the following years. To offer the analysis group with a complete NGS useful resource, we’ve developed the database Subsequent Era Sequencing Catalog (NGS Catalog, a frequently up to date database that collects, curates and manages obtainable human NGS information obtained from printed literature.
NGS Catalog deposits publication data of NGS research and their mutation traits (SNVs, small insertions/deletions, copy quantity variations, and structural variants), in addition to mutated genes and gene fusions detected by NGS. Different capabilities embody person information add, NGS basic evaluation pipelines, and NGS software program.
NGS Catalog is especially helpful for investigators who’re new to NGS however wish to benefit from these highly effective applied sciences for their very own analysis. Lastly, primarily based on the info deposited in NGS Catalog, we summarized options and findings from entire exome sequencing, entire genome sequencing, and transcriptome sequencing research for human ailments or traits.
Subsequent technology sequencing (NGS) methods for the genetic testing of myopathies.
Subsequent technology sequencing (NGS) applied sciences provide the chance to map total genomes at reasonably priced prices. This brings the genetic testing process to a better degree of complexity. The optimistic facet is the convenience to deal with the complicated analysis of genetically heterogeneous issues and to establish novel illness genes.
Worries come up from the administration of too many DNA variations with unpredictable that means and incidental findings that may trigger moral and medical dilemmas. The know-how of enrichment makes potential to focus the sequencing to the exome or to a extra particular DNA goal.

That is getting used to supply insights into the genetics underlying Mendelian traits concerned in myopathies and to arrange cost-effective diagnostic assessments. This large potential of the NGS purposes makes doubtless that these will quickly grow to be the primary strategy in genetic diagnostic laboratories.
EPA-ng: Massively Parallel Evolutionary Placement of Genetic Sequences.
Subsequent Era Sequencing (NGS) applied sciences have led to a ubiquity of molecular sequence information. This information avalanche is especially difficult in metagenetics, which focuses on taxonomic identification of sequences obtained from numerous microbial environments. Phylogenetic placement strategies decide how these sequences match into an evolutionary context. Earlier implementations of phylogenetic placement algorithms, such because the Evolutionary Placement Algorithm (EPA) included in RAxML, or pplacer, are being more and more used for this goal.
Nonetheless, as a result of regular progress in NGS applied sciences, the present implementations face substantial scalability limitations. Right here we current EPA-ng, a whole reimplementation of the EPA that’s considerably quicker, gives a distributed reminiscence parallelization, and integrates ideas from each, RAxML-EPA and pplacer. EPA-ng will be executed on customary shared reminiscence, in addition to on distributed reminiscence methods (e.g., computing clusters).
To show the scalability of EPA-ng we positioned 1 billion metagenetic reads from the Tara Oceans Undertaking onto a reference tree with 3;748 taxa in just below 7 hours, utilizing 2;048 cores. Our efficiency evaluation exhibits that EPA-ng outperforms RAxML-EPA and pplacer by as much as an element of 30 in sequential execution mode, whereas attaining comparable parallel effectivity on shared reminiscence methods. We additional present that the distributed reminiscence parallelization of EPA-ng scales effectively as much as 2;048 cores.
What next-generation sequencing (NGS) know-how has enabled us to study major autosomal recessive microcephaly (MCPH).
The affect that next-generation sequencing know-how (NGS) is having on many elements of molecular and cell biology, is changing into more and more obvious. One of the vital noticeable outcomes of the brand new know-how in human genetics, has been the accelerated fee of identification of disease-causing genes. Particularly for uncommon, heterogeneous issues, comparable to autosomal recessive major microcephaly (MCPH), the handful of genes beforehand recognized to harbour disease-causing mutations, has grown at an unprecedented fee inside a couple of years.
Data of recent genes mutated in MCPH during the last 4 years has contributed to our understanding of the dysfunction at each the medical and mobile ranges. The capabilities of proteins comparable to WDR62, CASC5, PHC1, CDK6, CENP-E, CENP-F, CEP63, ZNF335, PLK4 and TUBGPC, have been added to the complicated community of essential mobile processes recognized to be concerned in mind development and measurement.
Along with the significance of mitotic spindle meeting and construction, centrosome and centriole operate and DNA restore and harm response, new mechanisms involving kinetochore-associated proteins and chromatin remodelling complexes have been elucidated.
Magnesium oxide |
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GK6094-5KG | Glentham Life Sciences | 5 kg | 274.8 EUR |
Magnesium ribbon |
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GK9627-25G | Glentham Life Sciences | 25 g | 69.6 EUR |
Esomeprazole magnesium |
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HY-B1446 | MedChemExpress | 50mg | 129.6 EUR |
Saroglitazar Magnesium |
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HY-19937A | MedChemExpress | 5mg | 704.4 EUR |
Magnesium stearate |
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GX8335-100G | Glentham Life Sciences | 100 g | 52.8 EUR |
Magnesium stearate |
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GX8335-1KG | Glentham Life Sciences | 1 kg | 103.2 EUR |
Magnesium stearate |
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GX8335-250G | Glentham Life Sciences | 250 g | 60 EUR |
Magnesium stearate |
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GX8335-500G | Glentham Life Sciences | 500 g | 74.4 EUR |
Liposomal Magnesium |
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DPC002MG | ProFoldin | 2 ml | 140.62 EUR |
Description: This product includes 2 ml of 150 mM magnesium acetate encapsulated in liposomes. |
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MAGNESIUM CHLORIDE |
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313001 | Survival Technologies | each | Ask for price |
Magnesium Assay Kit |
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55R-1393 | Fitzgerald | 100 assays | 784.8 EUR |
Description: Assay Kit for detection of Magnesium in the research laboratory |
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Esomeprazole Magnesium trihydrate |
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A3399-100 | ApexBio | 100 mg | 537.6 EUR |
Description: Esomeprazole Magnesium (Nexium) is a proton pump inhibitor to reduce gastric acid secretion. The stability of Esomeprazole Magnesium is a function of pH; it rapidly degrades in acidic media, but it has acceptable stability under alkaline conditions. |
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Esomeprazole Magnesium trihydrate |
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A3399-200 | ApexBio | 200 mg | 710.4 EUR |
Description: Esomeprazole Magnesium (Nexium) is a proton pump inhibitor to reduce gastric acid secretion. The stability of Esomeprazole Magnesium is a function of pH; it rapidly degrades in acidic media, but it has acceptable stability under alkaline conditions. |
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Esomeprazole Magnesium trihydrate |
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A3399-50 | ApexBio | 50 mg | 340.8 EUR |
Description: Esomeprazole Magnesium (Nexium) is a proton pump inhibitor to reduce gastric acid secretion. The stability of Esomeprazole Magnesium is a function of pH; it rapidly degrades in acidic media, but it has acceptable stability under alkaline conditions. |
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Esomeprazole Magnesium trihydrate |
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A3399-500 | ApexBio | 500 mg | 1503.6 EUR |
Description: Esomeprazole Magnesium (Nexium) is a proton pump inhibitor to reduce gastric acid secretion. The stability of Esomeprazole Magnesium is a function of pH; it rapidly degrades in acidic media, but it has acceptable stability under alkaline conditions. |
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Magnesium Assay Kit |
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abx098445-Hitachi7020R140ml2R240ml2 | Abbexa | Hitachi 7020; R1: 40ml×2 R2: 40ml×2 | 247.2 EUR |
Magnesium Assay Kit |
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abx098445-Hitachi7060R190ml1R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×1 R2: 90ml×1 | 247.2 EUR |
Magnesium Assay Kit |
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abx098445-Toshiba40R140ml2R240ml2 | Abbexa | Toshiba 40; R1: 40ml×2 R2: 40ml×2 | 247.2 EUR |
Magnesium Assay Kit |
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abx098445-Toshiba40R150ml2R250ml2 | Abbexa | Toshiba 40; R1: 50ml×2 R2: 50ml×2 | 247.2 EUR |
Magnesium Assay Kit |
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abx298873-100Assays | Abbexa | 100 Assays | 510 EUR |
Magnesium acetate, tetrahydrate |
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MB0326 | Bio Basic | 500g | 82.97 EUR |
Magnesium chloride, hexahydrate |
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MB0328 | Bio Basic | 500g | 76.7 EUR |
Magnesium nitrate hexahydrate |
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MB0586 | Bio Basic | 500g | 80.88 EUR |
Magnesium dodecyl sulfate |
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MD1224 | Bio Basic | 25g | 101.76 EUR |
Magnesium chloride, hexahydrate |
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MRB0328 | Bio Basic | 250g | 73.57 EUR |
Magnesium sulfate, anhydrous |
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MN1988 | Bio Basic | 250g | 78.79 EUR |
Magnesium acetate tetrahydrate |
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GK4886-1KG | Glentham Life Sciences | 1 kg | 122.4 EUR |
Magnesium acetate tetrahydrate |
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GK4886-250G | Glentham Life Sciences | 250 g | 66 EUR |
Magnesium acetate tetrahydrate |
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GK4886-500G | Glentham Life Sciences | 500 g | 86.4 EUR |
Magnesium chloride, anhydrous |
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GK5046-100G | Glentham Life Sciences | 100 g | 55.2 EUR |
Magnesium chloride, anhydrous |
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GK5046-1KG | Glentham Life Sciences | 1 kg | 88.8 EUR |
Magnesium chloride, anhydrous |
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GK5046-500G | Glentham Life Sciences | 500 g | 67.2 EUR |
Magnesium chloride, anhydrous |
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GK5046-5KG | Glentham Life Sciences | 5 kg | 189.6 EUR |
Magnesium sulfate heptahydrate |
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GK8109-1KG | Glentham Life Sciences | 1 kg | 64.8 EUR |
Magnesium sulfate heptahydrate |
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GK8109-250G | Glentham Life Sciences | 250 g | 49.2 EUR |
Magnesium sulfate heptahydrate |
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GK8109-500G | Glentham Life Sciences | 500 g | 55.2 EUR |
Magnesium sulfate heptahydrate |
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GK8109-5KG | Glentham Life Sciences | 5 kg | 103.2 EUR |
Magnesium chloride hexahydrate |
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GK8228-1KG | Glentham Life Sciences | 1 kg | 60 EUR |
Magnesium chloride hexahydrate |
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GK8228-500G | Glentham Life Sciences | 500 g | 52.8 EUR |
Magnesium chloride hexahydrate |
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GK8228-5KG | Glentham Life Sciences | 5 kg | 103.2 EUR |
Magnesium sulfate, anhydrous |
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GK9094-100G | Glentham Life Sciences | 100 g | 52.8 EUR |
Magnesium sulfate, anhydrous |
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GK9094-1KG | Glentham Life Sciences | 1 kg | 103.2 EUR |
Magnesium sulfate, anhydrous |
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GK9094-250G | Glentham Life Sciences | 250 g | 64.8 EUR |
Magnesium sulfate, anhydrous |
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GK9094-500G | Glentham Life Sciences | 500 g | 79.2 EUR |
Magnesium sulfate, anhydrous |
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GK9094-5KG | Glentham Life Sciences | 5 kg | 265.2 EUR |
Esomeprazole (magnesium salt) |
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HY-17021A | MedChemExpress | 10mg | 886.8 EUR |
Esomeprazole (Magnesium trihydrate) |
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HY-17022 | MedChemExpress | 500mg | 1159.2 EUR |
(+)-Magnesium L-ascorbate |
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GV8858-100G | Glentham Life Sciences | 100 g | 84 EUR |
(+)-Magnesium L-ascorbate |
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GV8858-25G | Glentham Life Sciences | 25 g | 55.2 EUR |
Magnesium bromide hexahydrate |
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GX3683-100G | Glentham Life Sciences | 100 g | 60 EUR |
Magnesium bromide hexahydrate |
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GX3683-1KG | Glentham Life Sciences | 1 kg | 186 EUR |
Magnesium bromide hexahydrate |
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GX3683-250G | Glentham Life Sciences | 250 g | 84 EUR |
Magnesium bromide hexahydrate |
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GX3683-500G | Glentham Life Sciences | 500 g | 122.4 EUR |
Magnesium nitrate hexahydrate |
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GK0164-1KG | Glentham Life Sciences | 1 kg | 79.2 EUR |
Magnesium nitrate hexahydrate |
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GK0164-250G | Glentham Life Sciences | 250 g | 52.8 EUR |
Magnesium nitrate hexahydrate |
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GK0164-500G | Glentham Life Sciences | 500 g | 64.8 EUR |
Magnesium nitrate hexahydrate |
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GK0164-5KG | Glentham Life Sciences | 5 kg | 213.6 EUR |
Magnesium Assay Kit |
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Z5030032 | Biochain | 250 assays | 837.6 EUR |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Magnesium Chloride (MgCl?) |
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2105-100 | Biovision | each | 164.4 EUR |
Metal Slide Tray |
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T001 | Insitus Biotechnologies | each | 112.8 EUR |
Hard metal cutter |
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MIL1016 | Scientific Laboratory Supplies | EACH | 343.14 EUR |
Metal hub needle |
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DD74890 | Scientific Laboratory Supplies | PK6 | 194.94 EUR |
Metal hub needle |
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DD74934 | Scientific Laboratory Supplies | PK6 | 178.98 EUR |
Metal Grid Boxes |
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M-CEM-AGG1001 | MiTeGen | 1 UNIT | 47 EUR |
Description: Metal Grid Boxes |
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QuantiChrom Magnesium Assay Kit |
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DIMG-250 | BioAssay Systems | 250 | 472.8 EUR |
Description: Quantitative determination of magnesium ion Mg2+ by colorimetric (500nm) method. Procedure: 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM). Shelf life: 12 months. Shipping: ambient temp; storage: 4°C. |
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Magnesium sulfate, heptahydrate, ACS |
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MB0329 | Bio Basic | 500g | 74.62 EUR |
25mM MgCl2 (Magnesium chloride) |
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MB1193 | Bio Basic | 4x1.5ml, 6ml | 70.44 EUR |
Magnesium sulfate, heptahydrate, biotech |
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MRB0329 | Bio Basic | 500g | 76.7 EUR |
Magnesium citrate tribasic hydrate |
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GK3304-1KG | Glentham Life Sciences | 1 kg | 103.2 EUR |
Magnesium citrate tribasic hydrate |
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GK3304-500G | Glentham Life Sciences | 500 g | 74.4 EUR |
Magnesium D-gluconate hydrate |
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GC8065-100G | Glentham Life Sciences | 100 g | 79.2 EUR |
Magnesium D-gluconate hydrate |
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GC8065-250G | Glentham Life Sciences | 250 g | 111.6 EUR |
Magnesium D-gluconate hydrate |
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GC8065-25G | Glentham Life Sciences | 25 g | 57.6 EUR |
Magnesium D-gluconate hydrate |
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GC8065-500G | Glentham Life Sciences | 500 g | 165.6 EUR |
Magnesium Colorimetric Assay Kit |
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K2068-100 | ApexBio | 100 assays | 602.4 EUR |
Magnesium gluconate, USP grade |
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GX4157-100G | Glentham Life Sciences | 100 g | 112.8 EUR |
Magnesium gluconate, USP grade |
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GX4157-500G | Glentham Life Sciences | 500 g | 303.6 EUR |
Magnesium Chloride Stock Solution |
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MGCLS01 | Scientific Laboratory Supplies | 1L | 135.66 EUR |
Magnesium Colorimetric Assay Kit |
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K385-100 | Biovision | each | 574.8 EUR |
High Density Metal Free |
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6BCL-QH-100 | ABTBeads | 100 ml | 446.4 EUR |
High Density Metal Free |
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6BCL-QH-25 | ABTBeads | 25 ml | 194.4 EUR |
High Density Metal Free |
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6BCL-QH-500 | ABTBeads | 500 ml | 1564.8 EUR |
Low Density Metal Free |
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6BCL-QL-100 | ABTBeads | 100 ml | 446.4 EUR |
Low Density Metal Free |
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6BCL-QL-25 | ABTBeads | 25 ml | 194.4 EUR |
Low Density Metal Free |
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6BCL-QL-500 | ABTBeads | 500 ml | 1564.8 EUR |
Metal Free Rapid Run |
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6RR-QH-100 | ABTBeads | 100 ml | 517.2 EUR |
Metal Free Rapid Run |
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6RR-QH-25 | ABTBeads | 25 ml | 199.2 EUR |
Metal Free Rapid Run |
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6RR-QH-500 | ABTBeads | 500 ml | 906 EUR |
TPEN (Heavy metal chelator) |
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SIH-231-100MG | Stressmarq | 100 mg | 201.6 EUR |
Description: The substance TPEN is a heavy metal chelator. It is synthetically produced and has a purity of >98%. The pure substance is white to off-white solid which is soluble in DMSO, 100% ethanol or methanol. |
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TPEN (Heavy metal chelator) |
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SIH-231-500MG | Stressmarq | 500 mg | 560.4 EUR |
Description: The substance TPEN is a heavy metal chelator. It is synthetically produced and has a purity of >98%. The pure substance is white to off-white solid which is soluble in DMSO, 100% ethanol or methanol. |
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Desiccator Plate 90mm Metal |
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DES1050 | Scientific Laboratory Supplies | EACH | 10.26 EUR |
Desiccator Plate 140mm Metal |
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DES1052 | Scientific Laboratory Supplies | EACH | 20.52 EUR |
Desiccator Plate 184mm Metal |
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DES1054 | Scientific Laboratory Supplies | EACH | 27.36 EUR |
Desiccator Plate 235mm Metal |
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DES1056 | Scientific Laboratory Supplies | EACH | 35.34 EUR |
Desiccator Plate 280mm Metal |
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DES1058 | Scientific Laboratory Supplies | EACH | 44.46 EUR |
Metal Mirror Freezing Kit |
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M-CEM-37455 | MiTeGen | 1 UNIT | 3350 EUR |
Description: Metal Mirror Freezing Kit |
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Magnesium-Dependent Phosphatase 1 (Recombinant) |
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20-abx073181 | Abbexa |
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Magnesium Transporter NIPA4 (NIPA4) Antibody |
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abx025583-400ul | Abbexa | 400 ul | 627.6 EUR |
Magnesium Transporter NIPA4 (NIPA4) Antibody |
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abx025583-80l | Abbexa | 80 µl | 343.2 EUR |
Magnesium, powder, 150 - 350 mesh |
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GX0342-100G | Glentham Life Sciences | 100 g | 69.6 EUR |
EDTA magnesium disodium salt hydrate |
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GE3956-100G | Glentham Life Sciences | 100 g | 79.2 EUR |
EDTA magnesium disodium salt hydrate |
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GE3956-1KG | Glentham Life Sciences | 1 kg | 294 EUR |
EDTA magnesium disodium salt hydrate |
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GE3956-250G | Glentham Life Sciences | 250 g | 122.4 EUR |
EDTA magnesium disodium salt hydrate |
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GE3956-25G | Glentham Life Sciences | 25 g | 54 EUR |
EDTA magnesium disodium salt hydrate |
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GE3956-500G | Glentham Life Sciences | 500 g | 184.8 EUR |
Magnesium Std in Extracting Reagent |
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MGERS01 | Scientific Laboratory Supplies | 1L | 289.56 EUR |
MicroMolar Calcium / Magnesium Assay Kit |
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DMA200 | ProFoldin | 200 assays | 221.78 EUR |
Description: This product includes 200 ul of 100 x C56 dye and 1000 ul of 10 x Reagent E and 50 ul of CaCl2. It is for measurement of 200 samples using 96-well plates. |
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Metal Transporter CNNM3 (CNNM3) Antibody |
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20-abx111860 | Abbexa |
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Metal Transporter CNNM3 (CNNM3) Antibody |
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20-abx003478 | Abbexa |
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Metal Transporter CNNM4 (CNNM4) Antibody |
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abx032513-400ul | Abbexa | 400 ul | 627.6 EUR |
Metal Transporter CNNM4 (CNNM4) Antibody |
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abx032513-80l | Abbexa | 80 µl | 343.2 EUR |
Two of the foremost contributions to our medical data are the realisation that major microcephaly attributable to mutations in genes on the MCPH loci is seldom an remoted medical characteristic and is usually accompanied both by further cortical malformations or primordial dwarfism. Gene-phenotype correlations are being revisited, with a brand new dimension of locus heterogeneity and phenotypic variability being revealed.
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