gene knockout method
B2M Knockout THP-1 Cell Line |
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78389 | BPS Bioscience | 2 vials | 6500 EUR |
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from THP-1 cells. |
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CIITA Knockout THP-1 Cell Line |
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78390 | BPS Bioscience | 2 vials | 6500 EUR |
Description: CIITA (Class II Transactivator) has been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing. |
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TCR/B2M Knockout Jurkat Cell Line |
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78552 | BPS Bioscience | 2 vials | 8645 EUR |
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells to generate the TCR Knockout Jurkat cell Line (BPS Bioscience #78539). These TCR knockout cells were then used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. |
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FCGR2A (CD32A) Knockout Jurkat Cell Line |
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78549 | BPS Bioscience | 2 vials | 6500 EUR |
Description: The FCGR2A Knockout Jurkat cell line was generated by CRISPR/Cas9 genome editing to remove FCGR2A (CD32A), the gene encoding protein FcγRIIa (Fragment crystallizable gamma receptor II a, also known as FcGRIIa, Fc-gamma-RIIa, and CD32A). |
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B2M/CIITA Double Knockout THP-1 Cell Line |
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78391 | BPS Bioscience | 2 vials | 9500 EUR |
Description: Both B2M (Beta-2-Microglobulin) and CIITA (Class II Transactivator) have been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing. |
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B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line |
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78363 | BPS Bioscience | 2 vials | 11095 EUR |
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells. Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. |
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TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line |
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78556 | BPS Bioscience | 2 vials | 12205 EUR |
Description: This cell line is a knockout of TCR (T Cell Receptor). The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells stably expressing the firefly luciferase gene under the control of NFAT response elements.This cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to parental NFAT-Luciferase Reporter Jurkat cells (BPS Bioscience #60621). |
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TCR/B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line |
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78557 | BPS Bioscience | 2 vials | 16695 EUR |
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells to generate the TCR Knockout NFAT Luciferase Reporter Jurkat cell Line (BPS Bioscience #78556). These TCR knockout cells were used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. _x000D_Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. |
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CRISPR/Cas9 Kinase Knockout Lentivirus Library (Array Format) |
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78487 | BPS Bioscience | 200 µl x 649 | 7700 EUR |
Description: The CRISPR/Cas9 Kinase Knockout Lentivirus Library (Array Format) targets 619 human kinases and pseudo-kinases.bpsbioscience.com/media/wysiwyg/Kinases/Kinase_Library_-_List_Kinases_Pseudokinases_06-15-2022.xlsx Download the table to view all available kinases. The Array consists of a series of vials, with each vial containing a mixture of integrating CRISPR/Cas9 lentiviral particles targeting 5 sgRNAs for a specific gene (1 vial per gene, 5 sgRNAs per gene). The Array also includes a total of 150 control sgRNAs that do not target any gene (combined into 30 vials containing 5 control sgRNAs per vial). Thus, the Array contains a total of 649 vials and 3,245 sgRNAs.The lentiviruses are replication incompetent, VSV-G pseudotyped lentiviral particles ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The SIN (self-inactivation) lentiviral backbone contains the Cas9 gene (Streptococcus pyogenes CRISPR associated protein 9) driven by an EF1a promoter, an sgRNA driven by a U6 promoter, and a puromycin selection marker.The lentiviruses integrate randomly into the cellular genome to express both Cas9 and the sgRNAs. Because the lentiviruses contain Cas9, they can be used in any target cell regardless of whether the cells already express Cas9. Puromycin selection ensures high expression of both Cas9 and the sgRNAs. Knockout efficiencies will depend on the cell type and the gene of interest. Stable CRISPR/Cas9 knockout cell lines can also be generated following limiting dilution.The library is delivered with a User Manual booklet. |
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Gene Knock-Out HR Targeting Vector [MCS1-EF1α-RFP-T2A-Puro-pA-MCS2] |
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HR110PA-1 | SBI | 10 ug | 933 EUR |
Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2] |
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HR410PA-1 | SBI | 10 ug | 933 EUR |
Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP-T2A-Hygro-pA-MCS2] |
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HR510PA-1 | SBI | 10 ug | 933 EUR |
Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2] |
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HR210PA-1 | SBI | 10 ug | 1071 EUR |
Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration |
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HR700PA-1 | SBI | 10 µg | 1071 EUR |
Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration |
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HR710PA-1 | SBI | 10 µg | 1071 EUR |
Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration |
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HR720PA-1 | SBI | 10 µg | 1071 EUR |
Method 614 Kit |
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614-SK | Scientific Laboratory Supplies | 1ML | 706.8 EUR |
Method 524.3 Mix A |
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5243-G | Scientific Laboratory Supplies | 1ML | 254.4 EUR |
Method 525.3 Mix A |
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5253-A | Scientific Laboratory Supplies | 1ML | 358.8 EUR |
QuantiSir General Gene Knockdown Quantification Kit |
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P-5001 | EpiGentek |
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Method 525.3 PCB Mix |
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5253-PCB | Scientific Laboratory Supplies | 1ML | 282 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MXSTD-SETAN | Scientific Laboratory Supplies | EACH | 656.4 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MXSTD4A-100N | Scientific Laboratory Supplies | 125ML | 162 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MXSTD4A-500N | Scientific Laboratory Supplies | 500ML | 271.2 EUR |
US EPA Method 200.7 (Rev.3.3) and SW-846 Method 6010 (3rd ed.) |
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MIXSTD-SET | Scientific Laboratory Supplies | EACH | 768 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD-SETA | Scientific Laboratory Supplies | EACH | 703.2 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD1-100 | Scientific Laboratory Supplies | 125ML | 219.6 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD1-500 | Scientific Laboratory Supplies | 500ML | 363.6 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD1A-500 | Scientific Laboratory Supplies | 500ML | 409.2 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD1C-100 | Scientific Laboratory Supplies | 125ML | 240 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD1C-500 | Scientific Laboratory Supplies | 500ML | 408 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD2-100 | Scientific Laboratory Supplies | 125ML | 204 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD2-500 | Scientific Laboratory Supplies | 500ML | 398.4 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD2A-100 | Scientific Laboratory Supplies | 125ML | 176.4 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD2A-500 | Scientific Laboratory Supplies | 500ML | 342 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD3-100 | Scientific Laboratory Supplies | 125ML | 163.2 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD3-500 | Scientific Laboratory Supplies | 500ML | 274.8 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD3A-100 | Scientific Laboratory Supplies | 125ML | 142.8 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD3A-500 | Scientific Laboratory Supplies | 500ML | 234 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD4-100 | Scientific Laboratory Supplies | 125ML | 220.8 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD4-500 | Scientific Laboratory Supplies | 500ML | 366 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD4A-100 | Scientific Laboratory Supplies | 125ML | 186 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD4A-500 | Scientific Laboratory Supplies | 500ML | 304.8 EUR |
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD5-100 | Scientific Laboratory Supplies | 125ML | 206.4 EUR |
US EPA Method 200.7 (Rev.3.3) and SW-846 Method 6010 (3rd ed.) |
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MIXSTD5-500 | Scientific Laboratory Supplies | 500ML | 342 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD5A-100 | Scientific Laboratory Supplies | 125ML | 186 EUR |
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.) |
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MIXSTD5A-500 | Scientific Laboratory Supplies | 500ML | 304.8 EUR |
Method 8082 Calibration Mix |
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8082-C | Scientific Laboratory Supplies | 1ML | 286.8 EUR |
US EPA Method 200.7 (Revision 4.4) and SW-846 Method 6010(Third Edition) |
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MIXSTD1A-100 | Scientific Laboratory Supplies | 125ML | 241.2 EUR |
Method 531.2 Calibration Mix |
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5312-A | Scientific Laboratory Supplies | 1ML | 158.4 EUR |
Method 524.3 Supplemental Mix |
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5243-A | Scientific Laboratory Supplies | 1ML | 604.8 EUR |
EPA Method 525 INT STD |
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525-I | Scientific Laboratory Supplies | 1ML | 103.2 EUR |
Method 524.2 Revision 4 Mix |
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5242-R4200 | Scientific Laboratory Supplies | 1ML | 88.8 EUR |
EPA Method 8081 Pesticide standard |
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22-BIG-PEST-200 | Scientific Laboratory Supplies | 1ML | 76.8 EUR |
COD Reagent (1977 method) |
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WTR50W | Scientific Laboratory Supplies | 2.5L | 135.6 EUR |
Method 504.1 Analytes (High Level) |
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5041-AH | Scientific Laboratory Supplies | 1ML | 126 EUR |
EPA Method 5242 Mix with Gasses |
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5242-VCX-200G | Scientific Laboratory Supplies | 1ML | 158.4 EUR |
EPA Method 605 Calibration Standard |
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605-X2 | Scientific Laboratory Supplies | 1ML | 103.2 EUR |
Method 8121 Chlorinated Hydrocarbons QC Mix |
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8121-B | Scientific Laboratory Supplies | 1ML | 195.6 EUR |
Random Hexamers 100 µg |
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26-4000-03 | Gene Link | 100 ug | 150 EUR |
Description: Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3). References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5. |
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AGS8830-8 PCR instrument |
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AGS8830 | Daan Gene | 1 unit/set | Ask for price |
RNA/DNA Purification Kit (Magnetic Bead) |
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DA0623 | Daan Gene | 32 test/kit | Ask for price |
Specimen Preservation Reagent |
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DA0970 | Daan Gene | 100 test/kit | Ask for price |
PCR detection kit for Monkeypox Virus |
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DA1430 | Daan Gene | 24 test/kit | 129.6 EUR |
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970. |
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Nucleic Acid Instrument |
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Smart-32 | Daan Gene | 1 unit/set | Ask for price |
Nucleic Acid Instrument |
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Swift-96 | Daan Gene | 1 unit/set | Ask for price |
UOP Method 543 Standard-2 Components |
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UOP-543 | Scientific Laboratory Supplies | 1ML | 271.2 EUR |
UOP Method 551 Standard-7 Components |
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UOP-551 | Scientific Laboratory Supplies | 1ML | 370.8 EUR |
UOP Method 690 Standard-7 Components |
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UOP-690 | Scientific Laboratory Supplies | 1ML | 394.8 EUR |
UOP Method 744 Standard-8 Components |
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UOP-744 | Scientific Laboratory Supplies | 1ML | 430.8 EUR |