gene knockout method

May 14, 2020,

By Samantha

Method 614 Kit
614-SK	Scientific Laboratory Supplies	1ML	587.1 EUR
B2M Knockout THP-1 Cell Line
78389	BPS Bioscience	2 vials	6500 EUR
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from THP-1 cells.
CIITA Knockout THP-1 Cell Line
78390	BPS Bioscience	2 vials	6500 EUR
Description: CIITA (Class II Transactivator) has been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing.
FCGR2A (CD32A) Knockout Jurkat Cell Line
78549	BPS Bioscience	2 vials	6500 EUR
Description: The FCGR2A Knockout Jurkat cell line was generated by CRISPR/Cas9 genome editing to remove FCGR2A (CD32A), the gene encoding protein FcγRIIa (Fragment crystallizable gamma receptor II a, also known as FcGRIIa, Fc-gamma-RIIa, and CD32A).
TCR/B2M Knockout Jurkat Cell Line
78552	BPS Bioscience	2 vials	8645 EUR
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells to generate the TCR Knockout Jurkat cell Line (BPS Bioscience #78539). These TCR knockout cells were then used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing.
Method 524.3 Supplemental Mix
5243-A	Scientific Laboratory Supplies	1ML	498.18 EUR
Method 524.3 Mix A
5243-G	Scientific Laboratory Supplies	1ML	212.04 EUR
Method 525.3 Mix A
5253-A	Scientific Laboratory Supplies	1ML	292.98 EUR
Method 525.3 PCB Mix
5253-PCB	Scientific Laboratory Supplies	1ML	221.16 EUR
Method 531.2 Calibration Mix
5312-A	Scientific Laboratory Supplies	1ML	121.98 EUR
Method 8082 Calibration Mix
8082-C	Scientific Laboratory Supplies	1ML	225.72 EUR
COD Reagent (1977 method)
WTR50W	Scientific Laboratory Supplies	2.5L	109.44 EUR
Method 504.1 Analytes (High Level)
5041-AH	Scientific Laboratory Supplies	1ML	114 EUR
Method 524.2 Revision 4 Mix
5242-R4200	Scientific Laboratory Supplies	1ML	76.38 EUR
EPA Method 525 INT STD
525-I	Scientific Laboratory Supplies	1ML	92.34 EUR
EPA Method 605 Calibration Standard
605-X2	Scientific Laboratory Supplies	1ML	92.34 EUR
ASTM Method D6042 Internal Standard
ASTM-D6042-IS	Scientific Laboratory Supplies	1ML	320.34 EUR
EPA Method 8081 Pesticide standard
22-BIG-PEST-200	Scientific Laboratory Supplies	1ML	68.4 EUR
UHPLC Method Develop Kit 1.8um
CHR5148	Scientific Laboratory Supplies	PK5	3723.24 EUR
B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line
78363	BPS Bioscience	2 vials	11095 EUR
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells. Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.
B2M/CIITA Double Knockout THP-1 Cell Line
78391	BPS Bioscience	2 vials	9500 EUR
Description: Both B2M (Beta-2-Microglobulin) and CIITA (Class II Transactivator) have been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing.
CRISPR/Cas9 Kinase Knockout Lentivirus Library (Array Format)
78487	BPS Bioscience	200 µl x 649	7700 EUR
Description: The CRISPR/Cas9 Kinase Knockout Lentivirus Library (Array Format) targets 619 human kinases and pseudo-kinases.bpsbioscience.com/media/wysiwyg/Kinases/Kinase_Library_-_List_Kinases_Pseudokinases_06-15-2022.xlsx Download the table to view all available kinases. The Array consists of a series of vials, with each vial containing a mixture of integrating CRISPR/Cas9 lentiviral particles targeting 5 sgRNAs for a specific gene (1 vial per gene, 5 sgRNAs per gene). The Array also includes a total of 150 control sgRNAs that do not target any gene (combined into 30 vials containing 5 control sgRNAs per vial). Thus, the Array contains a total of 649 vials and 3,245 sgRNAs.The lentiviruses are replication incompetent, VSV-G pseudotyped lentiviral particles ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The SIN (self-inactivation) lentiviral backbone contains the Cas9 gene (Streptococcus pyogenes CRISPR associated protein 9) driven by an EF1a promoter, an sgRNA driven by a U6 promoter, and a puromycin selection marker.The lentiviruses integrate randomly into the cellular genome to express both Cas9 and the sgRNAs. Because the lentiviruses contain Cas9, they can be used in any target cell regardless of whether the cells already express Cas9. Puromycin selection ensures high expression of both Cas9 and the sgRNAs. Knockout efficiencies will depend on the cell type and the gene of interest. Stable CRISPR/Cas9 knockout cell lines can also be generated following limiting dilution.The library is delivered with a User Manual booklet.
TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line
78556	BPS Bioscience	2 vials	12205 EUR
Description: This cell line is a knockout of TCR (T Cell Receptor). The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRÎ±/Î² chains were genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells stably expressing the firefly luciferase gene under the control of NFAT response elements.This cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to parental NFAT-Luciferase Reporter Jurkat cells (BPS Bioscience #60621).
US EPA Method 200.7 (Rev.3.3) and SW-846 Method 6010 (3rd ed.)
MIXSTD-SET	Scientific Laboratory Supplies	EACH	595.08 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD-SETA	Scientific Laboratory Supplies	EACH	546.06 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD1-100	Scientific Laboratory Supplies	125ML	169.86 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD1-500	Scientific Laboratory Supplies	500ML	281.58 EUR
US EPA Method 200.7 (Revision 4.4) and SW-846 Method 6010(Third Edition)
MIXSTD1A-100	Scientific Laboratory Supplies	125ML	186.96 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD1A-500	Scientific Laboratory Supplies	500ML	315.78 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD1C-100	Scientific Laboratory Supplies	125ML	206.34 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD1C-500	Scientific Laboratory Supplies	500ML	351.12 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD2-100	Scientific Laboratory Supplies	125ML	156.18 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD2-500	Scientific Laboratory Supplies	500ML	306.66 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD2A-100	Scientific Laboratory Supplies	125ML	132.24 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD2A-500	Scientific Laboratory Supplies	500ML	262.2 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD3-100	Scientific Laboratory Supplies	125ML	126.54 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD3-500	Scientific Laboratory Supplies	500ML	208.62 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD3A-100	Scientific Laboratory Supplies	125ML	108.3 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD3A-500	Scientific Laboratory Supplies	500ML	180.12 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD4-100	Scientific Laboratory Supplies	125ML	169.86 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD4-500	Scientific Laboratory Supplies	500ML	281.58 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD4A-100	Scientific Laboratory Supplies	125ML	140.22 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD4A-500	Scientific Laboratory Supplies	500ML	234.84 EUR
US EPA Method 200.7 (Rev. 3.3) and SW-846 Method 6010 (3rd Ed.)
MIXSTD5-100	Scientific Laboratory Supplies	125ML	158.46 EUR
US EPA Method 200.7 (Rev.3.3) and SW-846 Method 6010 (3rd ed.)
MIXSTD5-500	Scientific Laboratory Supplies	500ML	262.2 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD5A-100	Scientific Laboratory Supplies	125ML	140.22 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MIXSTD5A-500	Scientific Laboratory Supplies	500ML	234.84 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MXSTD-SETAN	Scientific Laboratory Supplies	EACH	507.3 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MXSTD4A-100N	Scientific Laboratory Supplies	125ML	126.54 EUR
US EPA Method 200.7 (Rev. 4.4) and SW-846 Method 6010 (3rd Ed.)
MXSTD4A-500N	Scientific Laboratory Supplies	500ML	206.34 EUR
TCR/B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line
78557	BPS Bioscience	2 vials	16695 EUR
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRÎ±/Î² chains were genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells to generate the TCR Knockout NFAT Luciferase Reporter Jurkat cell Line (BPS Bioscience #78556). These TCR knockout cells were used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. _x000D_Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.
Antibody Pair Support Kit (Sandwich Method)
abx098958-1096tests	Abbexa	10 × 96 tests	343.2 EUR

Antibody Pair Support Kit (Competitive Method)
abx098959-1096tests	Abbexa	10 × 96 tests	343.2 EUR

EPA Method 5242 Mix with Gasses
5242-VCX-200G	Scientific Laboratory Supplies	1ML	140.22 EUR
Method 8121 Chlorinated Hydrocarbons QC Mix
8121-B	Scientific Laboratory Supplies	1ML	152.76 EUR
ASTM Method D2887 Standard-20 Components
ASTM-D2887	Scientific Laboratory Supplies	1ML	282.72 EUR
ASTM Method D3710 Standard-16 Components
ASTM-D3710	Scientific Laboratory Supplies	1ML	286.14 EUR
ASTM Method D6042 Standard-7 Components
ASTM-D6042	Scientific Laboratory Supplies	1ML	567.72 EUR
ASTM Method D6296 Standard-11 Components
ASTM-D6296-E	Scientific Laboratory Supplies	1ML	417.24 EUR
ASTM Method D6379 Standard-3 Components
ASTM-D6379	Scientific Laboratory Supplies	1ML	363.66 EUR
UOP Method 543 Standard-2 Components
UOP-543	Scientific Laboratory Supplies	1ML	226.86 EUR
UOP Method 551 Standard-7 Components
UOP-551	Scientific Laboratory Supplies	1ML	308.94 EUR
UOP Method 690 Standard-7 Components
UOP-690	Scientific Laboratory Supplies	1ML	328.32 EUR
UOP Method 744 Standard-8 Components
UOP-744	Scientific Laboratory Supplies	1ML	371.64 EUR
UOP Method 868 Standard-10 Components
UOP-868	Scientific Laboratory Supplies	1ML	421.8 EUR
UOP Method 931 Standard-5 Components
UOP-931	Scientific Laboratory Supplies	1ML	285 EUR
RNA/DNA Purification Kit (Magnetic Bead)
DA0623	Daan Gene	32 test/kit	Ask for price
Specimen Preservation Reagent
DA0970	Daan Gene	100 test/kit	Ask for price
PCR detection kit for Monkeypox Virus
DA1430	Daan Gene	24 test/kit	129.6 EUR
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970.
AGS8830-8 PCR instrument
AGS8830	Daan Gene	1 unit/set	Ask for price