B2M Knockout THP-1 Cell Line

78389 BPS Bioscience 2 vials 6500 EUR
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from THP-1 cells.

CIITA Knockout THP-1 Cell Line

78390 BPS Bioscience 2 vials 6500 EUR
Description: CIITA (Class II Transactivator) has been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing.

TCR/B2M Knockout Jurkat Cell Line

78552 BPS Bioscience 2 vials 8645 EUR
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells to generate the TCR Knockout Jurkat cell Line (BPS Bioscience #78539). These TCR knockout cells were then used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing.

FCGR2A (CD32A) Knockout Jurkat Cell Line

78549 BPS Bioscience 2 vials 6500 EUR
Description: The FCGR2A Knockout Jurkat cell line was generated by CRISPR/Cas9 genome editing to remove FCGR2A (CD32A), the gene encoding protein FcγRIIa (Fragment crystallizable gamma receptor II a, also known as FcGRIIa, Fc-gamma-RIIa, and CD32A).

B2M/CIITA Double Knockout THP-1 Cell Line

78391 BPS Bioscience 2 vials 9500 EUR
Description: Both B2M (Beta-2-Microglobulin) and CIITA (Class II Transactivator) have been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing.

B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

78363 BPS Bioscience 2 vials 11095 EUR
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells. Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.

TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line

78556 BPS Bioscience 2 vials 12205 EUR
Description: This cell line is a knockout of TCR (T Cell Receptor). The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells stably expressing the firefly luciferase gene under the control of NFAT response elements.This cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to parental NFAT-Luciferase Reporter Jurkat cells (BPS Bioscience #60621).

TCR/B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

78557 BPS Bioscience 2 vials 16695 EUR
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells to generate the TCR Knockout NFAT Luciferase Reporter Jurkat cell Line (BPS Bioscience #78556). These TCR knockout cells were used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. _x000D_Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. 

CRISPR/Cas9 Kinase Knockout Lentivirus Library (Array Format)

78487 BPS Bioscience 200 µl x 649 7700 EUR
Description: The CRISPR/Cas9 Kinase Knockout Lentivirus Library (Array Format) targets 619 human kinases and pseudo-kinases.bpsbioscience.com/media/wysiwyg/Kinases/Kinase_Library_-_List_Kinases_Pseudokinases_06-15-2022.xlsx Download the table to view all available kinases. The Array consists of a series of vials, with each vial containing a mixture of integrating CRISPR/Cas9 lentiviral particles targeting 5 sgRNAs for a specific gene (1 vial per gene, 5 sgRNAs per gene). The Array also includes a total of 150 control sgRNAs that do not target any gene (combined into 30 vials containing 5 control sgRNAs per vial). Thus, the Array contains a total of 649 vials and 3,245 sgRNAs.The lentiviruses are replication incompetent, VSV-G pseudotyped lentiviral particles ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The SIN (self-inactivation) lentiviral backbone contains the Cas9 gene (Streptococcus pyogenes CRISPR associated protein 9) driven by an EF1a promoter, an sgRNA driven by a U6 promoter, and a puromycin selection marker.The lentiviruses integrate randomly into the cellular genome to express both Cas9 and the sgRNAs. Because the lentiviruses contain Cas9, they can be used in any target cell regardless of whether the cells already express Cas9. Puromycin selection ensures high expression of both Cas9 and the sgRNAs. Knockout efficiencies will depend on the cell type and the gene of interest. Stable CRISPR/Cas9 knockout cell lines can also be generated following limiting dilution.The library is delivered with a User Manual booklet.

Gene Knock-Out HR Targeting Vector [MCS1-EF1α-RFP-T2A-Puro-pA-MCS2]

HR110PA-1 SBI 10 ug 933 EUR

Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2]

HR410PA-1 SBI 10 ug 933 EUR

Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP-T2A-Hygro-pA-MCS2]

HR510PA-1 SBI 10 ug 933 EUR

Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2]

HR210PA-1 SBI 10 ug 1071 EUR

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration

HR700PA-1 SBI 10 µg 1071 EUR

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration

HR710PA-1 SBI 10 µg 1071 EUR

Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration

HR720PA-1 SBI 10 µg 1071 EUR

JBS Floppy-Choppy In situ proteolysis as rescue technique in protein crystallization ** shipped on blue ice - must be shipped via overnight service

M-CO-110 MiTeGen 1 Kit 267 EUR
Description: JBS Floppy-Choppy In situ proteolysis as rescue technique in protein crystallization ** shipped on blue ice - must be shipped via overnight service

QuantiSir General Gene Knockdown Quantification Kit

P-5001 EpiGentek
  • 945.66 EUR
  • 376.20 EUR
  • 625.90 EUR
  • 96 Assays
  • 48 Assays
  • 96 Assays

Random Hexamers 100 µg

26-4000-03 Gene Link 100 ug 150 EUR
Description:

Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3).

References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

AGS8830-8 PCR instrument

AGS8830 Daan Gene 1 unit/set Ask for price

RNA/DNA Purification Kit (Magnetic Bead)

DA0623 Daan Gene 32 test/kit Ask for price

Specimen Preservation Reagent

DA0970 Daan Gene 100 test/kit Ask for price

PCR detection kit for Monkeypox Virus

DA1430 Daan Gene 24 test/kit 129.6 EUR
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970.

Nucleic Acid Instrument

Smart-32 Daan Gene 1 unit/set Ask for price

Nucleic Acid Instrument

Swift-96 Daan Gene 1 unit/set Ask for price

QuantiSir Specific Gene Knockdown Quantification Kit For DNA Damage/Repair - XRCC4

P-5003 EpiGentek 96 Assays 945.66 EUR

QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - TS

P-5005 EpiGentek 96 Assays 945.66 EUR

QuantiSir Specific Gene Knockdown Quantification Kit For Signal Transduction - ViDR

P-5008 EpiGentek 96 Assays 945.66 EUR

QuantiSir Specific Gene Knockdown Quantification Kit For Cell Death/Apoptosis - XIAP

P-5004 EpiGentek 96 Assays 945.66 EUR

QuantiSir Specific Gene Knockdown Quantification Kit For Cell Proliferation - Wnt 4

P-5006 EpiGentek 96 Assays 945.66 EUR

QuantiSir Specific Gene Knockdown Quantification Kit For Epigenetic Regulators - TFIIB

P-5002 EpiGentek 96 Assays 945.66 EUR

QuantiSir Specific Gene Knockdown Quantification Kit For Transcription Factor - YY-1

P-5009 EpiGentek 96 Assays 945.66 EUR

QuantiSir Specific Gene Knockdown Quantification Kit For Tumor Suppressor/Oncogene - WT-1

P-5007 EpiGentek 96 Assays 945.66 EUR

Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out

HR100PA-1 SBI 10 ug 868 EUR

TARGATT? Knock-in iPSC Generation (Master Cell Line)

AST-1100 Applied StemCell 1 vial of 1X10^6 cells Ask for price
Description: 6 month

TARGATT? Knock-in CHO Generation Kit (Master Cell Line)

AST-1200 Applied StemCell 1 Kit Ask for price
Description: 12 month

Difco Technical Agar

281230 Scientific Laboratory Supplies EACH 290.4 EUR

PH 7.01.00 Technical Buffer Sol

HI5007 Scientific Laboratory Supplies EACH 38.4 EUR

TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line)

AST-1300 Applied StemCell 1 Kit Ask for price
Description: 12 month

Technical Buffer pH 4.01 250ml

51350004 Scientific Laboratory Supplies EACH 30.6 EUR

Technical Buffer pH 10.10 250ml

51350010 Scientific Laboratory Supplies EACH 31.2 EUR

Pullulan, technical

GC6579-10 Glentham Life Sciences 10 46.6 EUR

Pullulan, technical

GC6579-25 Glentham Life Sciences 25 81.7 EUR

250ml Technical Buffer pH 2

51350002 Scientific Laboratory Supplies EACH 32.52 EUR

Technical Chlordane

CDANE-X Scientific Laboratory Supplies 1ML 96 EUR

Ellagic acid, 90%, technical

GP7432-10 Glentham Life Sciences 10 57.4 EUR

Stearic acid, technical, 90%

GK8631-1 Glentham Life Sciences 1 25.7 EUR

Stearic acid, technical, 90%

GK8631-100 Glentham Life Sciences 100 9.8 EUR

Stearic acid, technical, 90%

GK8631-5 Glentham Life Sciences 5 76.6 EUR

Heptane, technical grade

GK7040-1 Glentham Life Sciences 1 47.9 EUR

Heptane, technical grade

GK7040-2500 Glentham Life Sciences 2500 95.6 EUR

TARGATT? Knock-in Mouse Cell Line Generation Kit (Master Cell Line)

AST-7001 Applied StemCell 1 Kit Ask for price
Description: 6 month

Mettler technical pH buffer pH 7 (250ml)

51350006 Scientific Laboratory Supplies EACH 29.88 EUR

Barium carbonate, 95%, technical

GX0451-100G Glentham Life Sciences 100 g 52.8 EUR

Barium carbonate, 95%, technical

GX0451-10KG Glentham Life Sciences 10 kg 313.2 EUR

Barium carbonate, 95%, technical

GX0451-1KG Glentham Life Sciences 1 kg 84 EUR

Barium carbonate, 95%, technical

GX0451-500G Glentham Life Sciences 500 g 64.8 EUR

Barium carbonate, 95%, technical

GX0451-5KG Glentham Life Sciences 5 kg 189.6 EUR

Mettler technical pH buffer pH9.21 (250ml)

51350008 Scientific Laboratory Supplies EACH 31.08 EUR

Technical Chlordane (High Level)

CDANE-XH Scientific Laboratory Supplies 1ML 130.8 EUR

Calcium carbonate, technical

GK8162-25KG Glentham Life Sciences 25 kg 93.6 EUR

Calcium carbonate, technical

GK8162-5KG Glentham Life Sciences 5 kg 55.2 EUR

Calcium carbonate, technical

GK8162-25 Glentham Life Sciences 25 47.4 EUR

Calcium carbonate, technical

GK8162-5 Glentham Life Sciences 5 15.9 EUR

Mettler technical pH buffer pH11 (250ml)

51350012 Scientific Laboratory Supplies EACH 32.4 EUR

Gibco Bacto Yeast Extract Technical

288620 Scientific Laboratory Supplies EACH 94 EUR

Ammonia Solution 25% Technical Grade

NH25WW Scientific Laboratory Supplies 1L 277.2 EUR

Aluminium hydroxide, technical

GX7438-1 Glentham Life Sciences 1 52 EUR

Aluminium hydroxide, technical

GX7438-100 Glentham Life Sciences 100 17.9 EUR

Aluminium hydroxide, technical

GX7438-500 Glentham Life Sciences 500 32.6 EUR