Mapping the signal sequence-binding site on SRP reveals a significant role for the NG domain.

We current proof that the sign recognition particle (SRP) acknowledges sign sequences through the NG area on the SRP54 protein subunit. Utilizing a just lately developed cross-linking methodology (Fancy, D. A., and Kodadek, T. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 6020-6024; Correction (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 1317), we discover that sign peptides cross-link to the Escherichia coli SRP protein Ffh (the homologue of the mammalian SRP54 subunit) through the NG area.
Inside the NG area, the cross-linking web site maps to the ras-like C-terminal subdomain termed the G area. This consequence stands in distinction to earlier research, which concluded based mostly on nascent chain cross-linking that the sign sequence certain to the adjoining M area. As unbiased proof of a direct binding interplay between the NG area and the sign sequence, we discover that the NG area of Ffh binds sign peptides as an remoted entity. Our outcomes recommend that the NG area types a considerable a part of the binding web site for the sign sequence.
p53-Regulated Networks of Protein, mRNA, miRNA, and lncRNA Expression Revealed by Built-in Pulsed Secure Isotope Labeling With Amino Acids in Cell Tradition (pSILAC) and Subsequent Era Sequencing (NGS) Analyses.
We decided the impact of p53 activation on de novo protein synthesis utilizing quantitative proteomics (pulsed secure isotope labeling with amino acids in cell tradition/pSILAC) within the colorectal most cancers cell line SW480. This was mixed with mRNA and noncoding RNA expression analyses by subsequent technology sequencing (RNA-, miR-Seq). Moreover, genome-wide DNA binding of p53 was analyzed by chromatin-immunoprecipitation (ChIP-Seq).
Thereby, we recognized differentially regulated proteins (542 up, 569 down), mRNAs (1258 up, 415 down), miRNAs (111 up, 95 down) and lncRNAs (270 up, 123 down). Modifications in protein and mRNA expression ranges confirmed a constructive correlation (r = 0.50, p < 0.0001). In whole, we detected 133 direct p53 goal genes that had been differentially expressed and displayed p53 occupancy within the neighborhood of their promoter.
Extra transcriptionally induced genes displayed occupied p53 binding websites (4.3% mRNAs, 7.2% miRNAs, 6.3% lncRNAs, 5.9% proteins) than repressed genes (2.4% mRNAs, 3.2% miRNAs, 0.8% lncRNAs, 1.9% proteins), suggesting oblique mechanisms of repression. Round 50% of the down-regulated proteins displayed seed-matching sequences of p53-induced miRNAs within the corresponding 3′-UTRs.
Furthermore, proteins repressed by p53 considerably overlapped with these beforehand proven to be repressed by miR-34a. We confirmed up-regulation of the novel direct p53 goal genes LINC01021, MDFI, ST14 and miR-486 and confirmed that ectopic LINC01021 expression inhibits proliferation in SW480 cells. Moreover, KLF12, HMGB1 and CIT mRNAs had been confirmed as direct targets of the p53-induced miR-34a, miR-205 and miR-486-5p, respectively.

According to the lack of p53 operate throughout tumor development, elevated expression of KLF12, HMGB1 and CIT was detected in superior phases of most cancers. In conclusion, the combination of a number of omics strategies allowed the excellent identification of direct and oblique effectors of p53 that present new insights and leads into the mechanisms of p53-mediated tumor suppression.
Subsequent-generation sequencing (NGS) for evaluation of microbial water high quality: present progress, challenges, and future alternatives.
Water high quality is an emergent property of a posh system comprised of interacting microbial populations and launched microbial and chemical contaminants. Research leveraging next-generation sequencing (NGS) applied sciences are offering new insights into the ecology of microbially mediated processes that affect contemporary water high quality reminiscent of algal blooms, contaminant biodegradation, and pathogen dissemination. As well as, sequencing strategies focusing on small subunit (SSU) rRNA hypervariable areas have allowed identification of signature microbial species that function bioindicators for sewage contamination in these environments.
Past amplicon sequencing, metagenomic and metatranscriptomic analyses of microbial communities in contemporary water environments reveal the genetic capabilities and interaction of waterborne microorganisms, shedding gentle on the mechanisms for manufacturing and biodegradation of poisons and different contaminants. This evaluate discusses the challenges and advantages of making use of NGS-based strategies to water high quality analysis and evaluation.
We’ll contemplate the suitability and biases inherent within the software of NGS as a screening software for evaluation of organic dangers and talk about the potential and limitations for direct quantitative interpretation of NGS knowledge.
Secondly, we’ll study case research from latest literature the place NGS based mostly strategies have been utilized to matters in water high quality evaluation, together with growth of bioindicators for sewage air pollution and microbial supply monitoring, characterizing the distribution of toxin and antibiotic resistance genes in water samples, and investigating mechanisms of biodegradation of dangerous pollution that threaten water high quality. Lastly, we offer a brief evaluate of rising NGS platforms and their potential purposes to the subsequent technology of water high quality evaluation instruments.
Inference of Markovian properties of molecular sequences from NGS knowledge and purposes to comparative genomics.
Subsequent-generation sequencing (NGS) applied sciences generate massive quantities of quick learn knowledge for a lot of totally different organisms. The truth that NGS reads are typically quick makes it difficult to assemble the reads and reconstruct the unique genome sequence. For clustering genomes utilizing such NGS knowledge, word-count based mostly alignment-free sequence comparability is a promising method, however for this method, the underlying anticipated phrase counts are important
Tungsten Rod 1.2 mm, 99.95+% |
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GX0975-5 | Glentham Life Sciences | 5 | 216.7 EUR |
Tungsten Rod 2.5 mm, 99.95+% |
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GX1135-2 | Glentham Life Sciences | 2 | 113.1 EUR |
Tungsten Foil 0.2 mm, 99.96% |
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GX6917-100 | Glentham Life Sciences | 100 | 409 EUR |
Tungsten Foil 0.1 mm, 99.96% |
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GX7233-100 | Glentham Life Sciences | 100 | 391 EUR |
Tungsten Foil 0.5 mm, 99.96% |
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GX9553-100 | Glentham Life Sciences | 100 | 554 EUR |
Tungsten Foil 0.5 mm, 99.96% |
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GX9553-50 | Glentham Life Sciences | 50 | 203.6 EUR |
Tungsten Foil 0.15 mm, 99.96% |
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GX3609-100 | Glentham Life Sciences | 100 | 396 EUR |
Tungsten Foil 0.025 mm, 99.95% |
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GX3926-100 | Glentham Life Sciences | 100 | 482.2 EUR |
Tungsten Foil 0.025 mm, 99.95% |
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GX3926-45 | Glentham Life Sciences | 45 | 194 EUR |
Tungsten Foil 0.025 mm, 99.95% |
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GX3926-50 | Glentham Life Sciences | 50 | 321 EUR |
Tungsten Foil 1.0 mm, 99.96% |
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GX0201-100 | Glentham Life Sciences | 100 | 619.2 EUR |
Tungsten Foil 1.0 mm, 99.96% |
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GX0201-50 | Glentham Life Sciences | 50 | 252.7 EUR |
Tungsten Foil 0.7 mm, 99.96% |
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GX0404-100 | Glentham Life Sciences | 100 | 397.6 EUR |
Tungsten Foil 0.05 mm, 99.9+% |
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GX0695-100 | Glentham Life Sciences | 100 | 439.9 EUR |
Tungsten Foil 0.05 mm, 99.9+% |
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GX0695-25 | Glentham Life Sciences | 25 | 128.1 EUR |
Tungsten Foil 0.05 mm, 99.9+% |
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GX0695-50 | Glentham Life Sciences | 50 | 220.1 EUR |
Tungsten Foil 2.0 mm, 99.96% |
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GX1242-100 | Glentham Life Sciences | 100 | 536.1 EUR |
Tungsten Foil 2.0 mm, 99.96% |
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GX1242-50 | Glentham Life Sciences | 50 | 327.4 EUR |
Tungsten(VI) oxide, 99.9% |
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GX6170-100 | Glentham Life Sciences | 100 | 220.1 EUR |
Tungsten(VI) oxide, 99.9% |
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GX6170-25 | Glentham Life Sciences | 25 | 108.5 EUR |
Tungsten(VI) oxide, 99.995% |
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GX6962-10 | Glentham Life Sciences | 10 | 132.6 EUR |
Tungsten(VI) oxide, 99.995% |
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GX6962-25 | Glentham Life Sciences | 25 | 215.1 EUR |
Lead tungstate, 99.9% |
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GX7052-100 | Glentham Life Sciences | 100 | 197 EUR |
Lead tungstate, 99.9% |
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GX7052-25 | Glentham Life Sciences | 25 | 101 EUR |
Tungsten(VI) oxide, 99.99% |
|||
GX9024-100 | Glentham Life Sciences | 100 | 149.2 EUR |
Tungsten(VI) oxide, 99.99% |
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GX9024-250 | Glentham Life Sciences | 250 | 242.7 EUR |
Tungsten(IV) oxide, 99.5% |
|||
GX9957-100 | Glentham Life Sciences | 100 | 796.6 EUR |
Tungsten(IV) oxide, 99.5% |
|||
GX9957-25 | Glentham Life Sciences | 25 | 301.3 EUR |
Tungsten(IV) oxide, 99.5% |
|||
GX9957-5 | Glentham Life Sciences | 5 | 111.5 EUR |
Zinc tungstate, 99.9% |
|||
GX2338-10 | Glentham Life Sciences | 10 | 113.1 EUR |
Zinc tungstate, 99.9% |
|||
GX2338-25 | Glentham Life Sciences | 25 | 195.6 EUR |
Zinc tungstate, 99.9% |
|||
GX2338-50 | Glentham Life Sciences | 50 | 278.8 EUR |
Tungsten(VI) oxide, 99+% |
|||
GX0912-1 | Glentham Life Sciences | 1 | 309.6 EUR |
Tungsten(VI) oxide, 99+% |
|||
GX0912-100 | Glentham Life Sciences | 100 | 102.4 EUR |
Tungsten(VI) oxide, 99+% |
|||
GX0912-250 | Glentham Life Sciences | 250 | 131.1 EUR |
Tungsten(VI) oxide, 99.9% |
|||
GX1091-100 | Glentham Life Sciences | 100 | 113.1 EUR |
Tungsten(VI) oxide, 99.9% |
|||
GX1091-500 | Glentham Life Sciences | 500 | 254.1 EUR |
SKINNER LIST ACID EXTRACTABLES 1mL |
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SKINNER-AE | Scientific Laboratory Supplies | 1ML | 183.6 EUR |
Tungsten boride, 99.5% |
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GX6015-250 | Glentham Life Sciences | 250 | 472.4 EUR |
Tungsten boride, 99.5% |
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GX6015-50 | Glentham Life Sciences | 50 | 149.2 EUR |
Tungsten boride, 99.5% |
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GX2208-250 | Glentham Life Sciences | 250 | 472.4 EUR |
Tungsten boride, 99.5% |
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GX2208-50 | Glentham Life Sciences | 50 | 149.2 EUR |
Tungsten boride, 99+% |
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GX2331-25 | Glentham Life Sciences | 25 | 120.5 EUR |
Tungsten boride, 99+% |
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GX2331-250 | Glentham Life Sciences | 250 | 521.4 EUR |
Tungsten boride, 99+% |
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GX2331-50 | Glentham Life Sciences | 50 | 203.6 EUR |
Sodium tungstate |
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20-abx184826 | Abbexa |
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Tungsten sulfide, 99.8% |
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GX4566-100 | Glentham Life Sciences | 100 | 211.8 EUR |
Tungsten sulfide, 99.8% |
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GX4566-25 | Glentham Life Sciences | 25 | 104 EUR |
Tungsten sulfide, 99.8% |
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GX4566-250 | Glentham Life Sciences | 250 | 374.8 EUR |
Tungsten carbide |
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GX5611-100 | Glentham Life Sciences | 100 | 120.5 EUR |
Tungsten carbide |
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GX5611-250 | Glentham Life Sciences | 250 | 220.1 EUR |
Tungsten carbide |
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GX5611-500 | Glentham Life Sciences | 500 | 360.1 EUR |
Tungsten Pellets 6.35 x 6.35 mm, 99.97% |
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GX8345-100 | Glentham Life Sciences | 100 | 420.4 EUR |
Tungsten Pellets 6.35 x 6.35 mm, 99.97% |
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GX8345-25 | Glentham Life Sciences | 25 | 143.2 EUR |
Tungsten(IV) carbide, 99,95 % |
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GX8863-100 | Glentham Life Sciences | 100 | 353.6 EUR |
Tungsten(IV) carbide, 99,95 % |
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GX8863-25 | Glentham Life Sciences | 25 | 171.7 EUR |
Tungsten(IV) carbide, 99,95 % |
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GX8863-250 | Glentham Life Sciences | 250 | 596.8 EUR |
Tungsten(IV) carbide, 99,95 % |
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GX8863-500 | Glentham Life Sciences | 500 | 852.7 EUR |
Copper tungstate, 99.5% |
|||
GX9426-100 | Glentham Life Sciences | 100 | 262.3 EUR |
Copper tungstate, 99.5% |
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GX9426-25 | Glentham Life Sciences | 25 | 123.5 EUR |
Barium tungstate, 99.9% |
|||
GX1134-50 | Glentham Life Sciences | 50 | 198.7 EUR |
AAS Tungsten Std 1000ppm in H2O |
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AAW-H | Scientific Laboratory Supplies | 500ML | 166.8 EUR |
AAS Tungsten Std 10000ppm in H2O |
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AAW-M | Scientific Laboratory Supplies | 500ML | 144 EUR |
ICP Std Tungsten 1000ug/mL in 1% HNO3 + 2% HF |
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PW2A14 | Scientific Laboratory Supplies | 100ML | 221.95 EUR |
ICP Std Tungsten 1000ug/mL in 1% HNO3 + 2% HF |
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PW2B14 | Scientific Laboratory Supplies | 250ML | 651.6 EUR |
ICP Std Tungsten 10000ug/mL in 1% HNO3 + 2% HF |
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PW4B15 | Scientific Laboratory Supplies | 250ML | 485.68 EUR |
Lithium tungstate, 99.9% |
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GX5534-100 | Glentham Life Sciences | 100 | 215.1 EUR |
Lithium tungstate, 99.9% |
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GX5534-25 | Glentham Life Sciences | 25 | 107 EUR |
Calcium tungstate, 99+% |
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GX9049-100 | Glentham Life Sciences | 100 | 126.5 EUR |
Calcium tungstate, 99+% |
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GX9049-250 | Glentham Life Sciences | 250 | 246.1 EUR |
Tungsten silicide, 99% |
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GX9862-50 | Glentham Life Sciences | 50 | 180.9 EUR |
Tungsten Granules 0.5-2 mm, 99.7% |
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GX2321-1 | Glentham Life Sciences | 1 | 501.7 EUR |
Tungsten Granules 0.5-2 mm, 99.7% |
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GX2321-250 | Glentham Life Sciences | 250 | 201.1 EUR |
Beckman Tungsten Lamp |
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LT-BEC-100 | Scientific Laboratory Supplies | EACH | 58.8 EUR |
Jasco V500. 50 Tungsten Lamp |
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LT-JAS-102 | Scientific Laboratory Supplies | EACH | 58.8 EUR |
ICP Std Tungsten 1000ug/mL in 2% NH4OH |
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PW2A7 | Scientific Laboratory Supplies | 100ML | 221.95 EUR |
ICP Std Tungsten 1000ug/mL in 2% NH4OH |
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PW2B7 | Scientific Laboratory Supplies | 250ML | 359.04 EUR |
ICP Std Tungsten 1000ug/mL in 2% NH4OH |
|||
PW2C7 | Scientific Laboratory Supplies | 500ML | 489.6 EUR |
ICP Std Tungsten 10000ug/mL in 2% NH4OH |
|||
PW4A7 | Scientific Laboratory Supplies | 100ML | 386.46 EUR |
ICP Std Tungsten 10000ug/mL in 2% NH4OH |
|||
PW4B7 | Scientific Laboratory Supplies | 250ML | 462.18 EUR |
Rubidium tungstate, 99.9% |
|||
GX5881-10 | Glentham Life Sciences | 10 | 179.3 EUR |
Rubidium tungstate, 99.9% |
|||
GX5881-25 | Glentham Life Sciences | 25 | 311.3 EUR |
Tungsten Rod 20 mm diameter, 99.95+% |
|||
GX5958-100 | Glentham Life Sciences | 100 | 374.8 EUR |
Tungsten Rod 15 mm diameter, 99.95+% |
|||
GX8106-100 | Glentham Life Sciences | 100 | 255.7 EUR |
Tungsten Rod 12 mm diameter, 99.95+% |
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GX9070-150 | Glentham Life Sciences | 150 | 259.1 EUR |
Tungsten Rod 5.0 mm diameter, 99.95+% |
|||
GX3169-250 | Glentham Life Sciences | 250 | 120.5 EUR |
Tungsten Rod 10 mm diameter, 99.95+% |
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GX3271-150 | Glentham Life Sciences | 150 | 192.2 EUR |
Tungsten Rod 8.0 mm diameter, 99.95+% |
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GX3278-200 | Glentham Life Sciences | 200 | 171 EUR |
Tungsten Rod 8.0 mm diameter, 99.95+% |
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GX3278-500 | Glentham Life Sciences | 500 | 280.2 EUR |
Tungsten Rod 4.0 mm diameter, 99.95+% |
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GX0030-250 | Glentham Life Sciences | 250 | 107 EUR |
Tungsten Rod 3.0 mm diameter, 99.95+% |
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GX1465-500 | Glentham Life Sciences | 500 | 104 EUR |
Kinesis TSP uV100/1000/2000/3000 Tungsten Lamp |
|||
CHR6115 | Scientific Laboratory Supplies | EACH | 716.4 EUR |
Dionex Ultimate 3000 Tungsten Lamp |
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LT-DIO-105 | Scientific Laboratory Supplies | EACH | 387.6 EUR |
Tungsten Wire 0.5 mm diameter, 99.95+% |
|||
GX4458-10 | Glentham Life Sciences | 10 | 137.2 EUR |
Tungsten Wire 0.5 mm diameter, 99.95+% |
|||
GX4458-50 | Glentham Life Sciences | 50 | 332.3 EUR |
Tungsten Wire 0.1 mm diameter, 99.95+% |
|||
GX4882-100 | Glentham Life Sciences | 100 | 177.5 EUR |
Tungsten Wire 0.1 mm diameter, 99.95+% |
|||
GX4882-25 | Glentham Life Sciences | 25 | 92 EUR |
Manganese tungstate, 99.9% |
|||
GX4905-100 | Glentham Life Sciences | 100 | 304.8 EUR |
Manganese tungstate, 99.9% |
|||
GX4905-25 | Glentham Life Sciences | 25 | 125.1 EUR |
Tungsten Wire 1.4 mm diameter, 99.95+% |
|||
GX5029-10 | Glentham Life Sciences | 10 | 501.9 EUR |
Tungsten Wire 1.4 mm diameter, 99.95+% |
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GX5029-2 | Glentham Life Sciences | 2 | 167.9 EUR |
Tungsten Wire 0.15 mm diameter, 99.9% |
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GX6303-25 | Glentham Life Sciences | 25 | 119.1 EUR |
Tungsten Wire 0.4 mm diameter, 99.95+% |
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GX6591-10 | Glentham Life Sciences | 10 | 119.1 EUR |
Tungsten Wire 0.4 mm diameter, 99.95+% |
|||
GX6591-100 | Glentham Life Sciences | 100 | 355.3 EUR |
Tungsten Wire 0.4 mm diameter, 99.95+% |
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GX6591-50 | Glentham Life Sciences | 50 | 254.1 EUR |
Tungsten Nanopowder, 99,9 % |
|||
GX8256-10 | Glentham Life Sciences | 10 | 325.9 EUR |
Tungsten Nanopowder, 99,9 % |
|||
GX8256-25 | Glentham Life Sciences | 25 | 563.7 EUR |
Tungsten Powder 2.8 micron, 99.95% |
|||
GX8906-250 | Glentham Life Sciences | 250 | 131.1 EUR |
Tungsten Powder 2.8 micron, 99.95% |
|||
GX8906-500 | Glentham Life Sciences | 500 | 205.4 EUR |
Tungsten nanopowder, 99.0% |
|||
GX8914-10 | Glentham Life Sciences | 10 | 231.3 EUR |
Tungsten nanopowder, 99.0% |
|||
GX8914-100 | Glentham Life Sciences | 100 | 782.1 EUR |
Tungsten nanopowder, 99.0% |
|||
GX8914-25 | Glentham Life Sciences | 25 | 369.9 EUR |
Tungsten nanopowder, 99.0% |
|||
GX8914-5 | Glentham Life Sciences | 5 | 175.9 EUR |
Tungsten Wire 0.01 mm diameter, 99.9+% |
|||
GX9233-50 | Glentham Life Sciences | 50 | 563.7 EUR |
Tungsten Wire 0.3 mm diameter, 99.95+% |
|||
GX2248-100 | Glentham Life Sciences | 100 | 234.6 EUR |
Tungsten Wire 0.3 mm diameter, 99.95+% |
|||
GX2248-25 | Glentham Life Sciences | 25 | 110 EUR |
Tungsten Wire 0.7 mm diameter, 99.95+% |
|||
GX2815-25 | Glentham Life Sciences | 25 | 291.6 EUR |
Tungsten Wire 0.7 mm diameter, 99.95+% |
|||
GX2815-5 | Glentham Life Sciences | 5 | 123.5 EUR |
Tungsten Wire 0.25 mm diameter, 99.95+% |
|||
GX2906-25 | Glentham Life Sciences | 25 | 144.6 EUR |
Tungsten Wire 0.2 mm diameter, 99.95+% |
|||
GX3564-100 | Glentham Life Sciences | 100 | 246.1 EUR |
Tungsten Wire 0.2 mm diameter, 99.95+% |
|||
GX3564-25 | Glentham Life Sciences | 25 | 114.5 EUR |
Magnesium tungstate, 99.9% |
|||
GX3912-100 | Glentham Life Sciences | 100 | 325.9 EUR |
Magnesium tungstate, 99.9% |
|||
GX3912-25 | Glentham Life Sciences | 25 | 146.2 EUR |
Tungsten Wire 2.0 mm diameter, 99.95+% |
|||
GX0156-1 | Glentham Life Sciences | 1 | 166.2 EUR |
Tungsten Wire 2.0 mm diameter, 99.95+% |
|||
GX0156-5 | Glentham Life Sciences | 5 | 399 EUR |
Strontium tungstate, 99.9% |
|||
GX0472-10 | Glentham Life Sciences | 10 | 101 EUR |
Strontium tungstate, 99.9% |
|||
GX0472-50 | Glentham Life Sciences | 50 | 233 EUR |
Tungsten Wire 1.0 mm diameter, 99.95+% |
|||
GX0753-10 | Glentham Life Sciences | 10 | 490.3 EUR |
Tungsten Wire 1.0 mm diameter, 99.95+% |
|||
GX0753-2 | Glentham Life Sciences | 2 | 149.2 EUR |
Viral Nucleic Acid Extraction KitDNA/RNA |
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FYG401-100P | Yeastern Biotech | 100 Preps | Ask for price |
Viral Nucleic Acid Extraction KitDNA/RNA |
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FYG401-300P | Yeastern Biotech | 300 Preps | Ask for price |
Viral Nucleic Acid Extraction KitDNA/RNA |
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FYG401-50P | Yeastern Biotech | 50 Preps | Ask for price |
Tungsten Powder max. 10 micron, 99.99% |
|||
GX4644-100 | Glentham Life Sciences | 100 | 451.2 EUR |
Tungsten Powder 5 micron (Mo 1 ppm) |
|||
GX3247-GX324725g | Glentham Life Sciences | GX3247-25g | 417.1 EUR |
Tungsten Powder 5 micron (Mo 1 ppm) |
|||
GX3247-GX32475g | Glentham Life Sciences | GX3247-5g | 143.2 EUR |
Unicam 5625 5675 8600 8700 8800 Series Tungsten Lamp |
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LT-PHU-102 | Scientific Laboratory Supplies | EACH | 177.6 EUR |
Lamp Shimadzu Spec. Tungsten |
|||
IZ06265005AT | Scientific Laboratory Supplies | EACH | 49.74 EUR |
Tungsten(VI) oxide-Nano Powder, 99+% |
|||
GX7903-100 | Glentham Life Sciences | 100 | 1055.5 EUR |
Tungsten(VI) oxide-Nano Powder, 99+% |
|||
GX7903-25 | Glentham Life Sciences | 25 | 373.1 EUR |
Viral Nucleic Acid Extraction Kit II (50prep) |
|||
FAVNK-002 | Favorgen | 50 preps | 198 EUR |
Viral Nucleic Acid Extraction Kit II (100prep) |
|||
FAVNK-002-1 | Favorgen | 100 preps | 259.2 EUR |
Viral Nucleic Acid Extraction Kit II (300prep) |
|||
FAVNK-002-2 | Favorgen | 300 preps | 476.4 EUR |
Kinesis TSP uV6000 Tungsten Lamp |
|||
CHR6116 | Scientific Laboratory Supplies | EACH | 385.2 EUR |
Shimadzu M10AVP Tungsten Lamp |
|||
LT-SHI-104 | Scientific Laboratory Supplies | EACH | 57.6 EUR |
Sodium tungstate dihydrate |
|||
GK6152-100G | Glentham Life Sciences | 100 g | 79.2 EUR |
Sodium tungstate dihydrate |
|||
GK6152-250G | Glentham Life Sciences | 250 g | 122.4 EUR |
.A believable mannequin for this underlying distribution of phrase counts is given by means of modeling the DNA sequence as a Markov chain (MC). For single lengthy sequences, environment friendly statistics can be found to estimate the order of MCs and the transition chance matrix for the sequences. As NGS knowledge don’t present a single lengthy sequence, inference strategies on Markovian properties of sequences based mostly on single lengthy sequences can’t be instantly used for NGS quick learn knowledge.
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