Challenges in the introduction of next-generation sequencing (NGS) for diagnostics of myeloid malignancies into clinical routine use.
Given the huge phenotypic and genetic heterogeneity of acute and continual myeloid malignancies, hematologists have eagerly awaited the introduction of next-generation sequencing (NGS) into the routine diagnostic armamentarium to allow a extra differentiated illness classification, threat stratification, and improved therapeutic choices. At current, an rising variety of hematologic laboratories are within the technique of integrating NGS procedures into the diagnostic algorithms of sufferers with acute myeloid leukemia (AML), myelodysplastic syndromes (MDS), and myeloproliferative neoplasms (MPNs).
Inevitably accompanying such developments, physicians and molecular biologists are dealing with sudden challenges concerning the interpretation and implementation of molecular genetic outcomes derived from NGS in myeloid malignancies. This text summarizes typical challenges (Desk 1) which will come up within the context of NGS-based analyses at analysis and through follow-up of myeloid malignancies.
Desk 1 Challenges accompanying the introduction of large parallel sequencing in scientific routine diagnostics in hemato-oncology Problem Background Present and future method Discrimination of leukemia-related mutations from polymorphisms or passenger mutations Driver mutations anticipated to happen at increased allele frequency in affected person samples than passenger mutations; driver mutations extra prone to have an effect on protein perform than polymorphisms or passenger mutations Optimization of cancer-specific databases together with reporting of uncommon physiological gene variants Implementation of novel bioinformatic algorithms primarily based on prediction of useful influence Quantitative and dynamic.
VAF monitoring (individually and along with different mutations) at follow-up Discrimination of somatic leukemia-related mutations from CHIP CHIP is offered in ~10% of people aged 70 to 80 and in as much as 20% within the age group>> 80 years Quantitative and dynamic VAF monitoring (individually and along with different mutations) at follow-up Clarifying the importance of CHIP within the context of myeloid malignancies.
Discrimination of leukemia-related somatic mutations from pathogenic germline alterations Problem to distinguish acquired somatic mutations from germline pathogenic variants at analysis Mutation detection in germline management samples (e.g., pores and skin fibroblasts, saliva) in mutations corresponding to in RUNX1, CEBPA Thorough medical household historical past adopted by molecular genetic checks in kinfolk if essential Excessive and secure VAF (e.g., 40-50%) at follow-up regardless of scientific response to therapy could also be indicative for germline alteration.
Discrimination of true genetic alterations from PCR, sequencing and post-sequencing artifacts Many artefacts are identified to come up throughout NGS library preparation, sequencing and information evaluation Error correction utilizing molecular identifiers that individually label unique enter DNA molecules Refinement of error-correction computational strategies in post-sequencing NGS information evaluation Affirmation utilizing Sanger sequencing Restricted sensitivity of NGS for minimal residual illness (MRD) evaluation Mutations detected at analysis could also be re-identified at finest to a VAF of 1-2% Error-corrected sequencing utilizing molecular identifiers.
Complementation of NGS by established MRD instruments like real-time PCR and movement cytometry Excessive monetary burden; demand on interdisciplinary approaches Costly technical and workers gear, refined information interpretation Advanced translation of NGS outcomes into therapeutic choices Growth of repeatedly up to date NGS interpretation units and algorithms for well-established mutational profiles inside distinct hematological malignancies Interdisciplinary leukemia boards VAF variant allele frequency, CHIP clonal hematopoiesis of indeterminate significance, bp base pairs, G guanine, C cytosine, ITDs inside tandem duplication.
NGS-QCbox and Raspberry for Parallel, Automated and Fast High quality Management Evaluation of Massive-Scale Subsequent Technology Sequencing (Illumina) Knowledge.
Fast reputation and adaptation of subsequent technology sequencing (NGS) approaches have generated large volumes of knowledge. Excessive throughput platforms like Illumina HiSeq produce terabytes of uncooked information that requires fast processing. High quality management of the information is a crucial element previous to the downstream analyses. To deal with these points, we have now developed a high quality management pipeline, NGS-QCbox that scales as much as course of lots of or 1000’s of samples. Raspberry is an in-house device, developed in C language using HTSlib (v1.2.1) (http://htslib.org), for computing learn/base degree statistics.
It may be used as stand-alone utility and may course of each compressed and uncompressed FASTQ format recordsdata. NGS-QCbox integrates Raspberry with different open-source instruments for alignment (Bowtie2), SNP calling (SAMtools) and different utilities (bedtools) in the direction of analyzing uncooked NGS information at increased effectivity and in high-throughput method.
It stories learn and base statistics together with genome protection and variants in a consumer pleasant format. The pipeline developed presents a easy menu pushed interface and can be utilized in both fast or full mode. As well as, the pipeline in fast mode outperforms in pace in opposition to different related present QC pipeline/instruments.
Diminishing returns in next-generation sequencing (NGS) transcriptome information.
RNA-seq is more and more used to review gene expression of assorted organisms. Whereas it supplies an ideal alternative to discover genome-scale transcriptional patterns with large depth, it comes with prohibitive prices. Establishing a minimal sequencing depth for required accuracy will information cost-effective experimental design and promote the routine utility of RNA-seq. To deal with this subject, we chosen 36 RNA-seq datasets, every with greater than 20 million reads from six widely-used mannequin organisms: Saccharomyces cerevisiae, Homo sapiens, Drosophila melanogaster, Caenorhabditis elegans, Mus musculus, and Arabidopsis thaliana, and investigated statistical correlations between the sequencing depth and the result accuracy.
Diallyl disulfide |
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| TBZ1224 | ChemNorm | unit | Ask for price |
Carbon disulfide |
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| S-745 | Scientific Laboratory Supplies | 1ML | 38.76 EUR |
Methyl Disulfide |
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| S-2599 | Scientific Laboratory Supplies | 1ML | 72.96 EUR |
Kinesis Hollow Cathode Lamp Tungsten 37mm Std |
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| CHR6070 | Scientific Laboratory Supplies | EACH | 267.9 EUR |
Kinesis Hollow Cathode Lamp Tungsten 37mm unicam |
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| CHR6071 | Scientific Laboratory Supplies | EACH | 308.94 EUR |
Kinesis Hollow Cathode Lamp Tungsten 37mm Varian |
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| CHR6072 | Scientific Laboratory Supplies | EACH | 308.94 EUR |
Kinesis Hollow Cathode Lamp Tungsten 50mm Standard |
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| CHR6073 | Scientific Laboratory Supplies | EACH | 391.02 EUR |
Shimadzu SPD-10AV. AVP. AVVP. 20AV Tungsten Lamp |
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| LT-SHI-101 | Scientific Laboratory Supplies | EACH | 225.72 EUR |
ICP Std Tungsten 1000ug/mL in 2% NH4OH |
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| PW2A7 | Scientific Laboratory Supplies | 100ML | 177.84 EUR |
ICP Std Tungsten 1000ug/mL in 2% NH4OH |
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| PW2B7 | Scientific Laboratory Supplies | 250ML | 287.28 EUR |
ICP Std Tungsten 1000ug/mL in 2% NH4OH |
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| PW2C7 | Scientific Laboratory Supplies | 500ML | 392.16 EUR |
ICP Std Tungsten 10000ug/mL in 2% NH4OH |
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| PW4A7 | Scientific Laboratory Supplies | 100ML | 310.08 EUR |
ICP Std Tungsten 10000ug/mL in 2% NH4OH |
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| PW4B7 | Scientific Laboratory Supplies | 250ML | 370.5 EUR |
Kinesis TSP uV100/1000/2000/3000 Tungsten Lamp |
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| CHR6115 | Scientific Laboratory Supplies | EACH | 591.66 EUR |
Two (2) GAGG Sentinel Beamstop with Tungsten Housing |
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| DBS-GAGGBS-2-B | MiTeGen | 2 UNITS | 5358 EUR |
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Description: Two (2) GAGG Sentinel Beamstop with Tungsten Housing |
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Recombinant Disulfide Oxidoreductase |
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| 7-02692 | CHI Scientific | 10µg | Ask for price |
Recombinant Disulfide Oxidoreductase |
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| 7-02693 | CHI Scientific | 50µg | Ask for price |
Recombinant Disulfide Oxidoreductase |
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| 7-02694 | CHI Scientific | 1mg | Ask for price |
Disulfide Oxidoreductase (Recombinant) |
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| 20-abx073036 | Abbexa |
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Formamidine disulfide dihydrochloride |
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| GK5373-25G | Glentham Life Sciences | 25 g | 160.8 EUR |
LKB Pharmacia Ultrospec 2000 3000 Novaspec ii Tungsten Lamp |
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| LT-LKB-100 | Scientific Laboratory Supplies | EACH | 39.9 EUR |
Unicam 5625 5675 8600 8700 8800 Series Tungsten Lamp |
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| LT-PHU-102 | Scientific Laboratory Supplies | EACH | 145.92 EUR |
Kinesis Hollow Cathode Lamp Tungsten 50mm PE AAnalyst (Lumina) |
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| CHR6074 | Scientific Laboratory Supplies | EACH | 494.76 EUR |
Recombinant Disulfide Bond Isomerase |
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| 7-02695 | CHI Scientific | 10µg | Ask for price |
Recombinant Disulfide Bond Isomerase |
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| 7-02696 | CHI Scientific | 50µg | Ask for price |
Recombinant Disulfide Bond Isomerase |
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| 7-02697 | CHI Scientific | 1mg | Ask for price |
Protein disulfide-isomerase Antibody |
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| 20-abx110136 | Abbexa |
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Protein disulfide-isomerase Antibody |
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| 20-abx110137 | Abbexa |
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Disulfide Isomerase Protein (Recombinant) |
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| 20-abx073027 | Abbexa |
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Disulfide Bond Isomerase (Recombinant) |
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| 20-abx073037 | Abbexa |
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N1-Glutathionyl-spermidine disulfide |
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| H-1126.0005 | Bachem | 5.0mg | 544.8 EUR |
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Description: Sum Formula: C34H66N12O10S2; CAS# [108081-77-2] |
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N1-Glutathionyl-spermidine disulfide |
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| H-1126.0025 | Bachem | 25.0mg | 2067.6 EUR |
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Description: Sum Formula: C34H66N12O10S2; CAS# [108081-77-2] |
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Recombinant (E.Coli) Disulfide Oxidoreductase |
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| RP-365 | Alpha Diagnostics | 10 ug | 270 EUR |
Cecil Series 2. 1000 to 9000. CE Sereis Tungsten Lamp |
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| LT-CEC-111 | Scientific Laboratory Supplies | EACH | 87.78 EUR |
Unicam UV1 Helios Alpha. Beta Gamma and Delta Tungsten Lamp |
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| LT-PHU-103 | Scientific Laboratory Supplies | EACH | 112.86 EUR |
ICP Std Tungsten 1000ug/mL in 1% HNO3 + 2% HF |
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| PW2A14 | Scientific Laboratory Supplies | 100ML | 177.84 EUR |
ICP Std Tungsten 1000ug/mL in 1% HNO3 + 2% HF |
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| PW2B14 | Scientific Laboratory Supplies | 250ML | 525.54 EUR |
ICP Std Tungsten 10000ug/mL in 1% HNO3 + 2% HF |
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| PW4B15 | Scientific Laboratory Supplies | 250ML | 388.74 EUR |
Human Protein disulfide-isomerase (P4HB) |
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| 1-CSB-RP002544h | Cusabio |
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Description: Recombinant Human Protein disulfide-isomerase(P4HB),partial expressed in E.coli |
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Human Protein disulfide-isomerase (P4hb) |
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| 1-CSB-YP017342HU | Cusabio |
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Description: Recombinant Human Protein disulfide-isomerase(P4hb) expressed in Yeast |
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Human Protein disulfide-isomerase (P4HB) |
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| 1-CSB-YP017342HUf8 | Cusabio |
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Description: Recombinant Human Protein disulfide-isomerase(P4HB),partial expressed in Yeast |
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Human Protein disulfide-isomerase (P4HB) |
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| 1-CSB-EP017342HU | Cusabio |
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Description: Recombinant Human Protein disulfide-isomerase(P4HB) expressed in E.coli |
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Recombinant Human Protein Disulfide Isomerase |
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| 7-03304 | CHI Scientific | 100µg | Ask for price |
Recombinant Human Protein Disulfide Isomerase |
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| 7-03305 | CHI Scientific | 500µg | Ask for price |
Recombinant Human Protein Disulfide Isomerase |
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| 7-03306 | CHI Scientific | 1mg | Ask for price |
Protein disulfide-isomerase Polyclonal Antibody |
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| 42079-100ul | SAB | 100ul | 399.6 EUR |
Protein Disulfide Isomerase (PDI) Antibody |
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| 20-abx102689 | Abbexa |
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Protein Disulfide Isomerase (PDI) Antibody |
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| 20-abx102690 | Abbexa |
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Protein disulfide-isomerase Antibody (HRP) |
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| 20-abx108658 | Abbexa |
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Protein disulfide-isomerase Antibody (HRP) |
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| 20-abx108659 | Abbexa |
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Protein disulfide-isomerase TMX3 Antibody |
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| 20-abx110139 | Abbexa |
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Protein disulfide-isomerase Antibody (Biotin) |
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| 20-abx105821 | Abbexa |
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Protein disulfide-isomerase Antibody (Biotin) |
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| 20-abx105825 | Abbexa |
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Protein disulfide-isomerase Antibody (FITC) |
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| 20-abx107238 | Abbexa |
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Protein disulfide-isomerase Antibody (FITC) |
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| 20-abx107239 | Abbexa |
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Disulfide Isomerase A3 Protein (Recombinant) |
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| 20-abx073097 | Abbexa |
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Disulfide Isomerase A6 Protein (Recombinant) |
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| 20-abx073169 | Abbexa |
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Disulfide Isomerase A4 Protein (Recombinant) |
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| 20-abx073751 | Abbexa |
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Protein Disulfide Isomerase (PDI) Antibody |
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| 20-abx174245 | Abbexa |
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Protein Disulfide-Isomerase (P4HB) Antibody |
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| abx145769-100ug | Abbexa | 100 ug | 469.2 EUR |
Protein Disulfide Isomerase (PDI) Antibody |
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| 20-abx141570 | Abbexa |
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Protein Disulfide-Isomerase (P4HB) Antibody |
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| 20-abx126992 | Abbexa |
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Protein Disulfide Isomerase (PDI) Antibody |
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| 20-abx128556 | Abbexa |
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Protein Disulfide-Isomerase (P4HB) Antibody |
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| 20-abx000827 | Abbexa |
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Protein Disulfide-Isomerase (P4HB) Antibody |
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| abx031784-400ul | Abbexa | 400 ul | 627.6 EUR |
Protein Disulfide-Isomerase (P4HB) Antibody |
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| abx031784-80l | Abbexa | 80 µl | 343.2 EUR |
Protein Disulfide Isomerase (P4HB) Antibody |
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| 20-abx445165 | Abbexa |
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Protein Disulfide-Isomerase (P4HB) Antibody |
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| 20-abx300560 | Abbexa |
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Protein Disulfide Isomerase (PDI) Antibody |
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| abx236272-100ug | Abbexa | 100 ug | 577.2 EUR |
DsbA Disulfide Oxidoreductase Recombinant Protein |
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| PROTP0AEG4 | BosterBio | Regular: 50ug | 380.4 EUR |
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Description: Disulfide Oxidoreductase produced in E.Coli is a periplasmic protein isolated from E. coli, containing 208 amino acids having a molecular mass of 23,149 Dalton. ;The DsbA is purified by proprietary chromatographic techniques. |
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PDI, Protein Disulfide Isomerase, human |
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| RC512-13 | Bio Basic | 100ug | 307.96 EUR |
Recombinant Protein Disulfide Isomerase (PDI) |
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| 4-RPB061Hu01 | Cloud-Clone |
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Description: Recombinant Human Protein Disulfide Isomerase expressed in: E.coli |
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Recombinant Protein Disulfide Isomerase (PDI) |
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| 4-RPB061Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Protein Disulfide Isomerase expressed in: E.coli |
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Recombinant Protein Disulfide Isomerase (PDI) |
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| 4-RPB061Ra01 | Cloud-Clone |
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Description: Recombinant Rat Protein Disulfide Isomerase expressed in: E.coli |
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Recombinant (E.Coli) Disulfide Bond Isomerase |
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| RP-378 | Alpha Diagnostics | 10 ug | 270 EUR |
PDI (Protein Disulfide Isomerase) Antibody |
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| 5601-100 | Biovision | each | 379.2 EUR |
PDI (Protein Disulfide Isomerase) Antibody |
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| 5601-30T | Biovision | each | 175.2 EUR |
Cricetulus griseus Protein disulfide-isomerase (P4HB) |
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| 1-CSB-EP823188DXU | Cusabio |
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Description: Recombinant Cricetulus griseus Protein disulfide-isomerase(P4HB) expressed in E.coli |
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Human Protein disulfide-isomerase TMX3 (TMX3) |
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| 1-CSB-EP846645HU | Cusabio |
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Description: Recombinant Human Protein disulfide-isomerase TMX3(TMX3) expressed in E.coli |
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Human Protein Disulfide Isomerase ELISA kit |
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| E01P0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Human Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Protein Disulfide Isomerase ELISA kit |
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| E01P0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Human Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Protein Disulfide Isomerase ELISA kit |
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| E01P0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Human Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Protein disulfide-isomerase A2 (Pdia2) |
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| 1-CSB-YP017719MO | Cusabio |
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Description: Recombinant Mouse Protein disulfide-isomerase A2(Pdia2) expressed in Yeast |
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Cricetulus griseus Protein disulfide-isomerase (P4HB) |
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| 1-CSB-YP823188DXU | Cusabio |
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Description: Recombinant Cricetulus griseus Protein disulfide-isomerase(P4HB) expressed in Yeast |
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Anti-MSLN-disulfide linker-DM4 ADC |
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| ADC-W-426 | Creative Biolabs | 1mg | Ask for price |
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Description: This ADC product is comprised of an anti-MSLN monoclonal antibody conjugated via a disulfide linker to a DM4 |
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Goat Protein Disulfide Isomerase ELISA kit |
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| E06P0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Goat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Protein Disulfide Isomerase ELISA kit |
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| E06P0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Goat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Protein Disulfide Isomerase ELISA kit |
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| E06P0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Goat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Protein Disulfide Isomerase ELISA kit |
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| E08P0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Protein Disulfide Isomerase ELISA kit |
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| E08P0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Protein Disulfide Isomerase ELISA kit |
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| E08P0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Protein Disulfide Isomerase ELISA kit |
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| E04P0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Rabbit Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Protein Disulfide Isomerase ELISA kit |
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| E04P0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Rabbit Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Protein Disulfide Isomerase ELISA kit |
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| E04P0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Rabbit Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Protein Disulfide Isomerase ELISA kit |
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| E07P0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Porcine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Protein Disulfide Isomerase ELISA kit |
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| E07P0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Porcine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Protein Disulfide Isomerase ELISA kit |
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| E07P0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Porcine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Protein Disulfide Isomerase ELISA kit |
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| E03P0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Mouse Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Protein Disulfide Isomerase ELISA kit |
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| E03P0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Mouse Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Protein Disulfide Isomerase ELISA kit |
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| E03P0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Mouse Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Protein Disulfide Isomerase ELISA kit |
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| E02P0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Rat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Protein Disulfide Isomerase ELISA kit |
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| E02P0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Rat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Protein Disulfide Isomerase ELISA kit |
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| E02P0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Rat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Protein disulfide-isomerase Polyclonal Conjugated Antibody |
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| C42079 | SAB | 100ul | 476.4 EUR |
Mouse Protein disulfide-isomerase A2 (Pdia2) |
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| 1-CSB-EP017719MO | Cusabio |
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Description: Recombinant Mouse Protein disulfide-isomerase A2(Pdia2) expressed in E.coli |
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Human Protein disulfide-isomerase A3 (PDIA3) |
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| 1-CSB-EP017720HU | Cusabio |
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Description: Recombinant Human Protein disulfide-isomerase A3(PDIA3) expressed in E.coli |
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Recombinant E.Coli Thiol Disulfide Interchange Protein |
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| 7-06964 | CHI Scientific | 5µg | Ask for price |
Recombinant E.Coli Thiol Disulfide Interchange Protein |
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| 7-06965 | CHI Scientific | 25µg | Ask for price |
Recombinant E.Coli Thiol Disulfide Interchange Protein |
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| 7-06966 | CHI Scientific | 1mg | Ask for price |
Recombinant E.Coli Thiol Disulfide Interchange Protein |
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| 7-06967 | CHI Scientific | 5µg | Ask for price |
Recombinant E.Coli Thiol Disulfide Interchange Protein |
|||
| 7-06968 | CHI Scientific | 25µg | Ask for price |
Recombinant E.Coli Thiol Disulfide Interchange Protein |
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| 7-06969 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A3 |
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| 7-03307 | CHI Scientific | 5µg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A3 |
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| 7-03308 | CHI Scientific | 25µg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A3 |
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| 7-03309 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A4 |
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| 7-03310 | CHI Scientific | 2µg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A4 |
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| 7-03311 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A4 |
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| 7-03312 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A6 |
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| 7-03313 | CHI Scientific | 5µg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A6 |
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| 7-03314 | CHI Scientific | 20µg | Ask for price |
Recombinant Human Protein Disulfide Isomerase A6 |
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| 7-03315 | CHI Scientific | 1mg | Ask for price |
Protein Disulfide-Isomerase A6 Recombinant Protein |
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| 91-476 | ProSci | 0.05 mg | 651.3 EUR |
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Description: Protein Disulfide-Isomerase A6 (PDIA6) is a 48.5kDa protein that belongs to the protein disulfide isomerase family (PDI). PDIA6 is an enzyme in the endoplasmic reticulum in eukaryotes which catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. The PDIA6 expressed in platelets, its functions as a chaperone that inhibits aggregation of misfolded proteins. PDIA6 is part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1. PDIA6 also plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. |
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Protein Disulfide-Isomerase A4 Recombinant Protein |
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| 91-762 | ProSci | 0.05 mg | 651.3 EUR |
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Description: Protein Disulfide-Isomerase A4 (PDIA4) is an endoplasmic reticulum luminal protein that belongs to the protein disulfide isomerase family. Human PDIA4 is synthesized as a 625 amino acid precursor that contains a 20 amino acid signal sequence, and a 625 amino acid mature chain, including three thioredoxin domains. PDIA4 catalyzes the rearrangement of -S-S- bonds in proteins and is thought to be a deoxycytidine kinase. In addition, PDIA4 serves as a proteases protein disulfide isomerase, phospholipase or an arrangement of these. |
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Protein disulfide-isomerase A3 Recombinant Protein |
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| 91-869 | ProSci | 0.05 mg | 852.9 EUR |
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Description: PDIA3 protein is also known as Protein disulfide-isomerase A3. It is a protein that in humans is encoded by the PDIA3 gene.PDIA3 is an enzyme that belongs to the endoplasmic reticulum and interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. PDIA3 interacts with thiazide-sensitive sodium-chloride cotransporter in the kidney and is induced by glucose deprivation. PDIA3 is part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is important for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. |
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Protein disulfide-isomerase A5 Recombinant Protein |
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| 91-965 | ProSci | 0.05 mg | 852.9 EUR |
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Description: Protein disulfide-isomerase A5 is a 519 amino acids protein that belongs to the protein disulfide isomerase family.It contains 3 thioredoxin domains.It can catalyze the rearrangement of -S-S- bonds in proteins. |
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Protein disulfide-isomerase A2 Antibody (HRP) |
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| 20-abx108660 | Abbexa |
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Protein disulfide-isomerase A3 Antibody (HRP) |
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| 20-abx108661 | Abbexa |
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Protein disulfide-isomerase TMX3 Antibody (HRP) |
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| 20-abx108662 | Abbexa |
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Protein Disulfide-Isomerase A2 (Pdia2) Antibody |
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| 20-abx109353 | Abbexa |
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Protein Disulfide-Isomerase A2 (Pdia3) Antibody |
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| 20-abx110138 | Abbexa |
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Protein disulfide-isomerase A2 Antibody (Biotin) |
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| 20-abx105822 | Abbexa |
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Protein disulfide-isomerase A3 Antibody (Biotin) |
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| 20-abx105823 | Abbexa |
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Protein disulfide-isomerase TMX3 Antibody (Biotin) |
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| 20-abx105824 | Abbexa |
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Protein disulfide-isomerase A2 Antibody (FITC) |
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| 20-abx107240 | Abbexa |
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Protein disulfide-isomerase A3 Antibody (FITC) |
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| 20-abx107241 | Abbexa |
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Protein disulfide-isomerase TMX3 Antibody (FITC) |
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| 20-abx107242 | Abbexa |
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Human Protein Disulfide Isomerase (PDI) Protein |
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| 20-abx068737 | Abbexa |
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Rat Protein Disulfide Isomerase (PDI) Protein |
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| 20-abx068738 | Abbexa |
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Mouse Protein Disulfide Isomerase (PDI) Protein |
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| 20-abx068739 | Abbexa |
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Protein Disulfide Isomerase A2 (PDIA2) Antibody |
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| 20-abx103307 | Abbexa |
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Protein Disulfide Isomerase A3 (PDIA3) Antibody |
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| 20-abx103308 | Abbexa |
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Protein Disulfide Isomerase A2 (PDIA2) Antibody |
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| 20-abx174246 | Abbexa |
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Protein Disulfide Isomerase A3 (PDIA3) Antibody |
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| 20-abx174247 | Abbexa |
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Protein Disulfide Isomerase A4 (PDIA4) Antibody |
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| 20-abx174248 | Abbexa |
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Protein Disulfide Isomerase A5 (PDIA5) Antibody |
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| 20-abx174249 | Abbexa |
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Protein Disulfide Isomerase A6 (PDIA6) Antibody |
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| 20-abx174250 | Abbexa |
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Protein Disulfide-Isomerase (P4HB) Antibody Pair |
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| abx117373-1pair5x96wellplates | Abbexa | 1 pair (5x96 well plates) | 1212 EUR |
Protein Disulfide Isomerase A6 (PDIA6) Antibody |
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| 20-abx131442 | Abbexa |
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Protein Disulfide Isomerase A3 (PDIA3) Antibody |
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| 20-abx127001 | Abbexa |
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Protein Disulfide Isomerase A5 (PDIA5) Antibody |
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| 20-abx128192 | Abbexa |
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Protein Disulfide Isomerase A4 (PDIA4) Antibody |
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| 20-abx128208 | Abbexa |
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Protein Disulfide Isomerase A2 (PDIA2) Antibody |
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| 20-abx128942 | Abbexa |
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Protein Disulfide Isomerase A6 (PDIA6) Antibody |
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| 20-abx005339 | Abbexa |
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Protein Disulfide Isomerase A3 (PDIA3) Antibody |
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| 20-abx001008 | Abbexa |
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Protein Disulfide Isomerase A6 (PDIA6) Antibody |
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| 20-abx002406 | Abbexa |
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Protein Disulfide-Isomerase A4 (PDIA4) Antibody |
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| 20-abx003217 | Abbexa |
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Protein Disulfide Isomerase A5 (PDIA5) Antibody |
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| abx027813-400ul | Abbexa | 400 ul | 627.6 EUR |
Protein Disulfide Isomerase A5 (PDIA5) Antibody |
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| abx027813-80l | Abbexa | 80 µl | 343.2 EUR |
Protein Disulfide-Isomerase A4 (PDIA4) Antibody |
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| abx027892-400ul | Abbexa | 400 ul | 627.6 EUR |
Protein Disulfide-Isomerase A4 (PDIA4) Antibody |
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| abx027892-80l | Abbexa | 80 µl | 343.2 EUR |
Protein Disulfide Isomerase A6 (PDIA6) Antibody |
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| abx032922-400ul | Abbexa | 400 ul | 627.6 EUR |
Protein Disulfide Isomerase A6 (PDIA6) Antibody |
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| abx032922-80l | Abbexa | 80 µl | 343.2 EUR |
Protein Disulfide Isomerase A6 (PDIA6) Antibody |
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| abx032923-400ul | Abbexa | 400 ul | 627.6 EUR |
Protein Disulfide Isomerase A6 (PDIA6) Antibody |
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| abx032923-80l | Abbexa | 80 µl | 343.2 EUR |
Protein Disulfide Isomerase A3 (PDIA3) Antibody |
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| abx031794-400ul | Abbexa | 400 ul | 627.6 EUR |
Protein Disulfide Isomerase A3 (PDIA3) Antibody |
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| abx031794-80l | Abbexa | 80 µl | 343.2 EUR |
To realize this, we randomly selected reads from every dataset, mapped them to the reference genomes, and analyzed the accuracy achieved with various protection. Our outcomes indicated that as little as a million reads can present the identical sequencing accuracy in transcript abundance (r=0.99) as >30 million reads for highly-expressed genes in all six species. As a result of many metabolically and pathologically-relevant genes are extremely expressed, our findings is perhaps instructive for cost-effective experimental designs in NGS-based analysis and likewise present helpful steerage to related analysis for different organisms.
dna template strand sequencing
dna template strand to amino acid translation
dna template strand transcription
dna template strand translation
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