Challenges in the introduction of next-generation sequencing (NGS) for diagnostics of myeloid malignancies into clinical routine use.
Given the huge phenotypic and genetic heterogeneity of acute and continual myeloid malignancies, hematologists have eagerly awaited the introduction of next-generation sequencing (NGS) into the routine diagnostic armamentarium to allow a extra differentiated illness classification, threat stratification, and improved therapeutic choices. At current, an rising variety of hematologic laboratories are within the technique of integrating NGS procedures into the diagnostic algorithms of sufferers with acute myeloid leukemia (AML), myelodysplastic syndromes (MDS), and myeloproliferative neoplasms (MPNs).
Inevitably accompanying such developments, physicians and molecular biologists are dealing with sudden challenges concerning the interpretation and implementation of molecular genetic outcomes derived from NGS in myeloid malignancies. This text summarizes typical challenges (Desk 1) which will come up within the context of NGS-based analyses at analysis and through follow-up of myeloid malignancies.
Desk 1 Challenges accompanying the introduction of large parallel sequencing in scientific routine diagnostics in hemato-oncology Problem Background Present and future method Discrimination of leukemia-related mutations from polymorphisms or passenger mutations Driver mutations anticipated to happen at increased allele frequency in affected person samples than passenger mutations; driver mutations extra prone to have an effect on protein perform than polymorphisms or passenger mutations Optimization of cancer-specific databases together with reporting of uncommon physiological gene variants Implementation of novel bioinformatic algorithms primarily based on prediction of useful influence Quantitative and dynamic.
VAF monitoring (individually and along with different mutations) at follow-up Discrimination of somatic leukemia-related mutations from CHIP CHIP is offered in ~10% of people aged 70 to 80 and in as much as 20% within the age group>> 80 years Quantitative and dynamic VAF monitoring (individually and along with different mutations) at follow-up Clarifying the importance of CHIP within the context of myeloid malignancies.
Discrimination of leukemia-related somatic mutations from pathogenic germline alterations Problem to distinguish acquired somatic mutations from germline pathogenic variants at analysis Mutation detection in germline management samples (e.g., pores and skin fibroblasts, saliva) in mutations corresponding to in RUNX1, CEBPA Thorough medical household historical past adopted by molecular genetic checks in kinfolk if essential Excessive and secure VAF (e.g., 40-50%) at follow-up regardless of scientific response to therapy could also be indicative for germline alteration.
Discrimination of true genetic alterations from PCR, sequencing and post-sequencing artifacts Many artefacts are identified to come up throughout NGS library preparation, sequencing and information evaluation Error correction utilizing molecular identifiers that individually label unique enter DNA molecules Refinement of error-correction computational strategies in post-sequencing NGS information evaluation Affirmation utilizing Sanger sequencing Restricted sensitivity of NGS for minimal residual illness (MRD) evaluation Mutations detected at analysis could also be re-identified at finest to a VAF of 1-2% Error-corrected sequencing utilizing molecular identifiers.
Complementation of NGS by established MRD instruments like real-time PCR and movement cytometry Excessive monetary burden; demand on interdisciplinary approaches Costly technical and workers gear, refined information interpretation Advanced translation of NGS outcomes into therapeutic choices Growth of repeatedly up to date NGS interpretation units and algorithms for well-established mutational profiles inside distinct hematological malignancies Interdisciplinary leukemia boards VAF variant allele frequency, CHIP clonal hematopoiesis of indeterminate significance, bp base pairs, G guanine, C cytosine, ITDs inside tandem duplication.
NGS-QCbox and Raspberry for Parallel, Automated and Fast High quality Management Evaluation of Massive-Scale Subsequent Technology Sequencing (Illumina) Knowledge.
Fast reputation and adaptation of subsequent technology sequencing (NGS) approaches have generated large volumes of knowledge. Excessive throughput platforms like Illumina HiSeq produce terabytes of uncooked information that requires fast processing. High quality management of the information is a crucial element previous to the downstream analyses. To deal with these points, we have now developed a high quality management pipeline, NGS-QCbox that scales as much as course of lots of or 1000’s of samples. Raspberry is an in-house device, developed in C language using HTSlib (v1.2.1) (http://htslib.org), for computing learn/base degree statistics.
It may be used as stand-alone utility and may course of each compressed and uncompressed FASTQ format recordsdata. NGS-QCbox integrates Raspberry with different open-source instruments for alignment (Bowtie2), SNP calling (SAMtools) and different utilities (bedtools) in the direction of analyzing uncooked NGS information at increased effectivity and in high-throughput method.
It stories learn and base statistics together with genome protection and variants in a consumer pleasant format. The pipeline developed presents a easy menu pushed interface and can be utilized in both fast or full mode. As well as, the pipeline in fast mode outperforms in pace in opposition to different related present QC pipeline/instruments.
Diminishing returns in next-generation sequencing (NGS) transcriptome information.
RNA-seq is more and more used to review gene expression of assorted organisms. Whereas it supplies an ideal alternative to discover genome-scale transcriptional patterns with large depth, it comes with prohibitive prices. Establishing a minimal sequencing depth for required accuracy will information cost-effective experimental design and promote the routine utility of RNA-seq. To deal with this subject, we chosen 36 RNA-seq datasets, every with greater than 20 million reads from six widely-used mannequin organisms: Saccharomyces cerevisiae, Homo sapiens, Drosophila melanogaster, Caenorhabditis elegans, Mus musculus, and Arabidopsis thaliana, and investigated statistical correlations between the sequencing depth and the result accuracy.
Molybdenum(IV) disulfide Nanopowder, 99 % |
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| GX4987-25 | Glentham Life Sciences | 25 | 593.2 EUR |
Cobalt Nanopowder, 99,8 % |
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| GX4966-10 | Glentham Life Sciences | 10 | 252.7 EUR |
Cobalt Nanopowder, 99,8 % |
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| GX4966-100 | Glentham Life Sciences | 100 | 1052.7 EUR |
Cobalt Nanopowder, 99,8 % |
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| GX4966-25 | Glentham Life Sciences | 25 | 369.9 EUR |
Cobalt Nanopowder, 99,8 % |
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| GX4966-5 | Glentham Life Sciences | 5 | 195.6 EUR |
Silver Nanopowder, 99,9 % |
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| GX9825-10 | Glentham Life Sciences | 10 | 264 EUR |
Silver Nanopowder, 99,9 % |
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| GX9825-100 | Glentham Life Sciences | 100 | 1046 EUR |
Silver Nanopowder, 99,9 % |
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| GX9825-25 | Glentham Life Sciences | 25 | 428.6 EUR |
Silver Nanopowder, 99,9 % |
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| GX9825-5 | Glentham Life Sciences | 5 | 208.6 EUR |
Copper Nanopowder, 99,9 % |
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| GX1781-10 | Glentham Life Sciences | 10 | 194.7 EUR |
Copper Nanopowder, 99,9 % |
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| GX1781-100 | Glentham Life Sciences | 100 | 575.7 EUR |
Copper Nanopowder, 99,9 % |
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| GX1781-25 | Glentham Life Sciences | 25 | 291.1 EUR |
Copper Nanopowder, 99,9 % |
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| GX1781-5 | Glentham Life Sciences | 5 | 158.2 EUR |
Silver Nanopowder, 99.95 % |
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| GX4085-10 | Glentham Life Sciences | 10 | 375.8 EUR |
Silver Nanopowder, 99.95 % |
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| GX4085-100 | Glentham Life Sciences | 100 | 1522.5 EUR |
Silver Nanopowder, 99.95 % |
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| GX4085-25 | Glentham Life Sciences | 25 | 675.2 EUR |
Silver Nanopowder, 99.5 % |
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| GX0585-10 | Glentham Life Sciences | 10 | 387.8 EUR |
Silver Nanopowder, 99.5 % |
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| GX0585-100 | Glentham Life Sciences | 100 | 1518.6 EUR |
Silver Nanopowder, 99.5 % |
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| GX0585-5 | Glentham Life Sciences | 5 | 275.2 EUR |
Silicon Nanopowder, 99+ % |
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| GX8061-10 | Glentham Life Sciences | 10 | 252.7 EUR |
Silicon Nanopowder, 99+ % |
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| GX8061-100 | Glentham Life Sciences | 100 | 1079.3 EUR |
Silicon Nanopowder, 99+ % |
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| GX8061-25 | Glentham Life Sciences | 25 | 424.8 EUR |
Tantalum Nanopowder, 99+ % |
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| GX3404-10 | Glentham Life Sciences | 10 | 325.9 EUR |
Tantalum Nanopowder, 99+ % |
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| GX3404-25 | Glentham Life Sciences | 25 | 546 EUR |
Tantalum Nanopowder, 99+ % |
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| GX3404-50 | Glentham Life Sciences | 50 | 752.9 EUR |
Titanium Nanopowder, 99,9 % |
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| GX3635-10 | Glentham Life Sciences | 10 | 294.9 EUR |
Titanium Nanopowder, 99,9 % |
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| GX3635-100 | Glentham Life Sciences | 100 | 1153.6 EUR |
Titanium Nanopowder, 99,9 % |
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| GX3635-25 | Glentham Life Sciences | 25 | 475.8 EUR |
Graphite Nanopowder, 99.9 % |
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| GX0508-10 | Glentham Life Sciences | 10 | 175.9 EUR |
Graphite Nanopowder, 99.9 % |
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| GX0508-100 | Glentham Life Sciences | 100 | 599.6 EUR |
Graphite Nanopowder, 99.9 % |
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| GX0508-500 | Glentham Life Sciences | 500 | 1646.9 EUR |
Iron Nanopowder, 99.7% [50nm] |
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| GX0385-10 | Glentham Life Sciences | 10 | 245.2 EUR |
Iron Nanopowder, 99.7% [50nm] |
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| GX0385-100 | Glentham Life Sciences | 100 | 1044.1 EUR |
Iron Nanopowder, 99.7% [50nm] |
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| GX0385-25 | Glentham Life Sciences | 25 | 444.5 EUR |
Zinc oxide, Nanopowder, 99.9% |
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| GX7801-100 | Glentham Life Sciences | 100 | 171 EUR |
Zinc oxide, Nanopowder, 99.9% |
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| GX7801-50 | Glentham Life Sciences | 50 | 111.5 EUR |
Zinc oxide, Nanopowder, 99.5% |
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| GX9146-100 | Glentham Life Sciences | 100 | 238.8 EUR |
Zinc oxide, Nanopowder, 99.5% |
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| GX9146-250 | Glentham Life Sciences | 250 | 476.6 EUR |
Zinc oxide, Nanopowder, 99.5% |
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| GX9146-50 | Glentham Life Sciences | 50 | 141 EUR |
Copper nanopowder, 99.5% [50nm] |
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| GX2223-10 | Glentham Life Sciences | 10 | 245.2 EUR |
Copper nanopowder, 99.5% [50nm] |
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| GX2223-100 | Glentham Life Sciences | 100 | 901.7 EUR |
Copper nanopowder, 99.5% [50nm] |
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| GX2223-25 | Glentham Life Sciences | 25 | 376.5 EUR |
Tin(IV) oxide Nanopowder, 99.9 % |
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| GX4773-1 | Glentham Life Sciences | 1 | 1370.1 EUR |
Tin(IV) oxide Nanopowder, 99.9 % |
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| GX4773-250 | Glentham Life Sciences | 250 | 475.8 EUR |
Tin(IV) oxide Nanopowder, 99.9 % |
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| GX4773-50 | Glentham Life Sciences | 50 | 187.5 EUR |
Tin(IV) oxide Nanopowder, 99.5% |
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| GX8443-100 | Glentham Life Sciences | 100 | 466 EUR |
Tin(IV) oxide Nanopowder, 99.5% |
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| GX8443-25 | Glentham Life Sciences | 25 | 187.5 EUR |
Boron nitride Nanopowder, 99.0% |
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| GX5901-10 | Glentham Life Sciences | 10 | 252.7 EUR |
Boron nitride Nanopowder, 99.0% |
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| GX5901-5 | Glentham Life Sciences | 5 | 192.2 EUR |
Barium titanate Nanopowder, 100nm, 99.9% |
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| GX4731-1 | Glentham Life Sciences | 1 | 633.8 EUR |
Barium titanate Nanopowder, 100nm, 99.9% |
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| GX4731-250 | Glentham Life Sciences | 250 | 226.6 EUR |
Barium titanate Nanopowder, 100nm, 99.9% |
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| GX4731-50 | Glentham Life Sciences | 50 | 114.5 EUR |
Barium titanate Nanopowder, 99,95 % |
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| GX3373-1 | Glentham Life Sciences | 1 | 1075.4 EUR |
Barium titanate Nanopowder, 99,95 % |
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| GX3373-100 | Glentham Life Sciences | 100 | 252.7 EUR |
Barium titanate Nanopowder, 99,95 % |
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| GX3373-250 | Glentham Life Sciences | 250 | 296.5 EUR |
Barium titanate Nanopowder, 99,95 % |
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| GX3373-50 | Glentham Life Sciences | 50 | 144.6 EUR |
Cerium(IV) oxide, nanopowder, 99.95% |
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| GX5310-25 | Glentham Life Sciences | 25 | 187.5 EUR |
Copper(II) oxide Nanopowder, 99,9 % |
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| GX2222-250 | Glentham Life Sciences | 250 | 385.7 EUR |
Copper(II) oxide Nanopowder, 99,9 % |
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| GX2222-50 | Glentham Life Sciences | 50 | 149.3 EUR |
Nickel Nanopowder, 99.8%, APS 20nm |
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| GX0127-10 | Glentham Life Sciences | 10 | 252.7 EUR |
Tin nanopowder, 99.9% [APS LT 100nm] |
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| GX7490-10 | Glentham Life Sciences | 10 | 194.7 EUR |
Tin nanopowder, 99.9% [APS LT 100nm] |
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| GX7490-100 | Glentham Life Sciences | 100 | 615.3 EUR |
Tin nanopowder, 99.9% [APS LT 100nm] |
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| GX7490-25 | Glentham Life Sciences | 25 | 302.2 EUR |
Silicon(IV) oxide Nanopowder, 99%+ |
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| GX5961-1 | Glentham Life Sciences | 1 | 1016.7 EUR |
Silicon(IV) oxide Nanopowder, 99%+ |
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| GX5961-250 | Glentham Life Sciences | 250 | 344 EUR |
Silicon(IV) oxide Nanopowder, 99%+ |
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| GX5961-50 | Glentham Life Sciences | 50 | 129.6 EUR |
Strontium titanate Nanopowder, 99.9% |
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| GX7135-1 | Glentham Life Sciences | 1 | 633.8 EUR |
Strontium titanate Nanopowder, 99.9% |
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| GX7135-100 | Glentham Life Sciences | 100 | 192.2 EUR |
Strontium titanate Nanopowder, 99.9% |
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| GX7135-250 | Glentham Life Sciences | 250 | 281.9 EUR |
Strontium titanate Nanopowder, 99.9% |
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| GX7135-50 | Glentham Life Sciences | 50 | 116.1 EUR |
Yttrium(III) oxide Nanopowder, 99,95 % |
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| GX8397-1 | Glentham Life Sciences | 1 | 1182.9 EUR |
Yttrium(III) oxide Nanopowder, 99,95 % |
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| GX8397-100 | Glentham Life Sciences | 100 | 221.7 EUR |
Yttrium(III) oxide Nanopowder, 99,95 % |
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| GX8397-250 | Glentham Life Sciences | 250 | 389.5 EUR |
Yttrium(III) oxide Nanopowder, 99,95 % |
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| GX8397-50 | Glentham Life Sciences | 50 | 129.6 EUR |
Graphene Nanopowder, 99.5 %, 11 - 15 nm |
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| GX6750-100 | Glentham Life Sciences | 100 | 482.9 EUR |
Graphene Nanopowder, 99.5 %, 11 - 15 nm |
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| GX6750-25 | Glentham Life Sciences | 25 | 208.7 EUR |
Graphene Nanopowder, 99.5 %, 11 - 15 nm |
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| GX6750-250 | Glentham Life Sciences | 250 | 964.8 EUR |
Graphene Nanopowder, 99.5 %, 6 - 8 nm |
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| GX3231-100 | Glentham Life Sciences | 100 | 488.9 EUR |
Graphene Nanopowder, 99.5 %, 6 - 8 nm |
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| GX3231-25 | Glentham Life Sciences | 25 | 244.3 EUR |
Graphene Nanopowder, 99.5 %, 6 - 8 nm |
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| GX3231-250 | Glentham Life Sciences | 250 | 733.2 EUR |
Boron carbide nanopowder, 50nm, 99% |
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| GX3429-10 | Glentham Life Sciences | 10 | 294.9 EUR |
Boron carbide nanopowder, 50nm, 99% |
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| GX3429-100 | Glentham Life Sciences | 100 | 1370.1 EUR |
Boron carbide nanopowder, 50nm, 99% |
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| GX3429-25 | Glentham Life Sciences | 25 | 487.2 EUR |
Boron carbide nanopowder, 50nm, 99% |
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| GX3429-5 | Glentham Life Sciences | 5 | 192.2 EUR |
Chromium(III) oxide Nanopowder, 99+ % |
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| GX0244-25 | Glentham Life Sciences | 25 | 144.6 EUR |
Chromium(III) oxide Nanopowder, 99+ % |
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| GX0244-250 | Glentham Life Sciences | 250 | 669.8 EUR |
Samarium(III) oxide Nanopowder, 99,9 % |
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| GX0514-100 | Glentham Life Sciences | 100 | 261.2 EUR |
Samarium(III) oxide Nanopowder, 99,9 % |
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| GX0514-250 | Glentham Life Sciences | 250 | 389.9 EUR |
Samarium(III) oxide Nanopowder, 99,9 % |
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| GX0514-50 | Glentham Life Sciences | 50 | 149.3 EUR |
Neodymium(III) oxide Nanopowder, 99,9 % |
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| GX5515-100 | Glentham Life Sciences | 100 | 602.6 EUR |
Graphene Nanopowder, 99.5 %, approx. 2nm |
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| GX7364-100 | Glentham Life Sciences | 100 | 325.9 EUR |
Graphene Nanopowder, 99.5 %, approx. 2nm |
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| GX7364-25 | Glentham Life Sciences | 25 | 175.9 EUR |
Graphene Nanopowder, 99.5 %, approx. 2nm |
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| GX7364-250 | Glentham Life Sciences | 250 | 415.5 EUR |
Stainless steel 316L Nanopowder, 99,9 % |
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| GX7816-10 | Glentham Life Sciences | 10 | 407.4 EUR |
Stainless steel 316L Nanopowder, 99,9 % |
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| GX7816-100 | Glentham Life Sciences | 100 | 1694.5 EUR |
Stainless steel 316L Nanopowder, 99,9 % |
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| GX7816-25 | Glentham Life Sciences | 25 | 723.4 EUR |
Stainless steel 316L Nanopowder, 99,9 % |
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| GX7816-5 | Glentham Life Sciences | 5 | 294.9 EUR |
gamma-Iron(III) oxide nanopowder |
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| GX8246-100 | Glentham Life Sciences | 100 | 309.6 EUR |
gamma-Iron(III) oxide nanopowder |
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| GX8246-25 | Glentham Life Sciences | 25 | 123.5 EUR |
Titanium(IV) carbide Nanopowder, 99 % |
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| GX0735-10 | Glentham Life Sciences | 10 | 140.2 EUR |
Titanium(IV) carbide Nanopowder, 99 % |
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| GX0735-100 | Glentham Life Sciences | 100 | 557.7 EUR |
Titanium(IV) carbide Nanopowder, 99 % |
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| GX0735-25 | Glentham Life Sciences | 25 | 217.9 EUR |
Titanium(IV) carbide Nanopowder, 99 % |
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| GX0735-50 | Glentham Life Sciences | 50 | 281.9 EUR |
Manganese(III) oxide Nanopowder, 98+ % |
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| GX1319-10 | Glentham Life Sciences | 10 | 146.2 EUR |
Manganese(III) oxide Nanopowder, 98+ % |
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| GX1319-100 | Glentham Life Sciences | 100 | 840.8 EUR |
Manganese(III) oxide Nanopowder, 98+ % |
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| GX1319-50 | Glentham Life Sciences | 50 | 599.6 EUR |
Silicon(IV) carbide Nanopowder (beta) |
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| GX4183-GX4183100g | Glentham Life Sciences | GX4183-100g | 487.2 EUR |
Silicon(IV) carbide Nanopowder (beta) |
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| GX4183-GX4183250g | Glentham Life Sciences | GX4183-250g | 793.5 EUR |
Silicon(IV) carbide Nanopowder (beta) |
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| GX4183-GX418325g | Glentham Life Sciences | GX4183-25g | 221.7 EUR |
Silicon(IV) carbide Nanopowder (beta) |
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| GX4183-GX4183500g | Glentham Life Sciences | GX4183-500g | 1458.3 EUR |
Silicon(IV) carbide Nanopowder (beta) |
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| GX3494-GX349410g | Glentham Life Sciences | GX3494-10g | 140.2 EUR |
Silicon(IV) carbide Nanopowder (beta) |
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| GX3494-GX349425g | Glentham Life Sciences | GX3494-25g | 210.1 EUR |
Titanium(III) nitride Nanopowder, 97 % |
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| GX0376-10 | Glentham Life Sciences | 10 | 163 EUR |
Titanium(III) nitride Nanopowder, 97 % |
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| GX0376-100 | Glentham Life Sciences | 100 | 666.8 EUR |
Titanium(III) nitride Nanopowder, 97 % |
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| GX0376-25 | Glentham Life Sciences | 25 | 259.8 EUR |
Titanium(III) nitride Nanopowder, 97 % |
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| GX0376-50 | Glentham Life Sciences | 50 | 369.9 EUR |
Titanium(III) nitride Nanopowder, 97 % |
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| GX0376-500 | Glentham Life Sciences | 500 | 1782.5 EUR |
Aluminium(III) nitride Nanopowder, 99 % |
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| GX5582-100 | Glentham Life Sciences | 100 | 598.3 EUR |
Aluminium(III) nitride Nanopowder, 99 % |
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| GX5582-25 | Glentham Life Sciences | 25 | 253.2 EUR |
Aluminium(III) nitride Nanopowder, 99 % |
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| GX5582-50 | Glentham Life Sciences | 50 | 354.9 EUR |
Aluminium(III) nitride Nanopowder, 99 % |
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| GX3387-100 | Glentham Life Sciences | 100 | 487.2 EUR |
Aluminium(III) nitride Nanopowder, 99 % |
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| GX3387-25 | Glentham Life Sciences | 25 | 239.6 EUR |
Aluminium(III) nitride Nanopowder, 99 % |
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| GX3387-50 | Glentham Life Sciences | 50 | 280.2 EUR |
Titanium oxide Nanopowder, rutile, 99,5 % |
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| GX3990-1 | Glentham Life Sciences | 1 | 1005.2 EUR |
Titanium oxide Nanopowder, rutile, 99,5 % |
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| GX3990-100 | Glentham Life Sciences | 100 | 221.7 EUR |
Titanium oxide Nanopowder, rutile, 99,5 % |
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| GX3990-250 | Glentham Life Sciences | 250 | 358.4 EUR |
Titanium oxide Nanopowder, rutile, 99,5 % |
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| GX3990-50 | Glentham Life Sciences | 50 | 129.6 EUR |
Titanium oxide Nanopowder, anatase, 99,5% |
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| GX7199-1 | Glentham Life Sciences | 1 | 1005.2 EUR |
Titanium oxide Nanopowder, anatase, 99,5% |
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| GX7199-100 | Glentham Life Sciences | 100 | 221.7 EUR |
Titanium oxide Nanopowder, anatase, 99,5% |
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| GX7199-250 | Glentham Life Sciences | 250 | 392.6 EUR |
Titanium oxide Nanopowder, anatase, 99,5% |
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| GX7199-50 | Glentham Life Sciences | 50 | 129.6 EUR |
alpha-Aluminium oxide Nanopowder, 99,5 % |
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| GX4775-100 | Glentham Life Sciences | 100 | 252.7 EUR |
alpha-Aluminium oxide Nanopowder, 99,5 % |
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| GX4775-50 | Glentham Life Sciences | 50 | 144.6 EUR |
gamma-Aluminium oxide, nanopowder, 99.95% |
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| GX1999-25 | Glentham Life Sciences | 25 | 143.2 EUR |
alpha-Aluminium oxide Nanopowder, 99,9 % |
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| GX2399-1 | Glentham Life Sciences | 1 | 633.8 EUR |
alpha-Aluminium oxide Nanopowder, 99,9 % |
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| GX2399-250 | Glentham Life Sciences | 250 | 259.1 EUR |
alpha-Aluminium oxide Nanopowder, 99,9 % |
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| GX2399-50 | Glentham Life Sciences | 50 | 114.5 EUR |
alpha-Aluminium oxide Nanopowder, 99+ % |
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| GX2901-1 | Glentham Life Sciences | 1 | 694 EUR |
gamma-Aluminium oxide Nanopowder, 99,9 % |
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| GX3420-1 | Glentham Life Sciences | 1 | 782.1 EUR |
gamma-Aluminium oxide Nanopowder, 99,9 % |
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| GX3420-250 | Glentham Life Sciences | 250 | 280.2 EUR |
gamma-Aluminium oxide Nanopowder, 99,9 % |
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| GX3420-50 | Glentham Life Sciences | 50 | 125.1 EUR |
alpha-Aluminium oxide Nanopowder, 99,85 % |
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| GX1021-1 | Glentham Life Sciences | 1 | 487.2 EUR |
alpha-Aluminium oxide Nanopowder, 99,85 % |
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| GX1021-250 | Glentham Life Sciences | 250 | 216.7 EUR |
alpha-Aluminium oxide Nanopowder, 99,85 % |
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| GX1021-50 | Glentham Life Sciences | 50 | 101 EUR |
Boron nitride Nanopowder, 99.0% [APS 70 nm] |
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| GX5307-10 | Glentham Life Sciences | 10 | 140.2 EUR |
Boron nitride Nanopowder, 99.0% [APS 70 nm] |
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| GX5307-100 | Glentham Life Sciences | 100 | 417.1 EUR |
Boron nitride Nanopowder, 99.0% [APS 70 nm] |
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| GX5307-25 | Glentham Life Sciences | 25 | 180.9 EUR |
Boron nitride Nanopowder, 99.0% [APS 70 nm] |
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| GX5307-50 | Glentham Life Sciences | 50 | 299.9 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
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| GX7444-GX7444100g | Glentham Life Sciences | GX7444-100g | 270.4 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
|||
| GX7444-GX7444250g | Glentham Life Sciences | GX7444-250g | 436.6 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
|||
| GX7444-GX744425g | Glentham Life Sciences | GX7444-25g | 140.2 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
|||
| GX7444-GX7444500g | Glentham Life Sciences | GX7444-500g | 782.1 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
|||
| GX7444-GX744450g | Glentham Life Sciences | GX7444-50g | 164.5 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
|||
| GX3828-GX3828100g | Glentham Life Sciences | GX3828-100g | 369.9 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
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| GX3828-GX3828250g | Glentham Life Sciences | GX3828-250g | 606.1 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
|||
| GX3828-GX382825g | Glentham Life Sciences | GX3828-25g | 192.2 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
|||
| GX3828-GX3828500g | Glentham Life Sciences | GX3828-500g | 1106.4 EUR |
Silicon(IV) nitride Nanopowder (amorphous) |
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| GX3828-GX382850g | Glentham Life Sciences | GX3828-50g | 216.7 EUR |
alpha-Iron(III) oxide Nanopowder (Hämatite) |
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| GX7168-GX7168100g | Glentham Life Sciences | GX7168-100g | 339 EUR |
Magnesium oxide Nanopowder + Stearic Acid, 99,9 % |
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| GX8585-1 | Glentham Life Sciences | 1 | 1811.7 EUR |
Magnesium oxide Nanopowder + Stearic Acid, 99,9 % |
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| GX8585-25 | Glentham Life Sciences | 25 | 140.2 EUR |
Magnesium oxide Nanopowder + Stearic Acid, 99,9 % |
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| GX8585-250 | Glentham Life Sciences | 250 | 585 EUR |
Magnesium oxide Nanopowder + Stearic Acid, 99,9 % |
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| GX8585-50 | Glentham Life Sciences | 50 | 211.8 EUR |
Silicon(IV) oxide Nanopowder non-porous, 99.5 % |
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| GX3021-100 | Glentham Life Sciences | 100 | 299.9 EUR |
Silicon(IV) oxide Nanopowder non-porous, 99.5 % |
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| GX3021-50 | Glentham Life Sciences | 50 | 224.9 EUR |
Silver Nanopowder, coated with fatty acids, 99+ % |
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| GX4779-10 | Glentham Life Sciences | 10 | 387.8 EUR |
To realize this, we randomly selected reads from every dataset, mapped them to the reference genomes, and analyzed the accuracy achieved with various protection. Our outcomes indicated that as little as a million reads can present the identical sequencing accuracy in transcript abundance (r=0.99) as >30 million reads for highly-expressed genes in all six species. As a result of many metabolically and pathologically-relevant genes are extremely expressed, our findings is perhaps instructive for cost-effective experimental designs in NGS-based analysis and likewise present helpful steerage to related analysis for different organisms.
dna template strand sequencing
dna template strand to amino acid translation
dna template strand transcription
dna template strand translation
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