Given the huge phenotypic and genetic heterogeneity of acute and continual myeloid malignancies, hematologists have eagerly awaited the introduction of next-generation sequencing (NGS) into the routine diagnostic armamentarium to allow a extra differentiated illness classification, threat stratification, and improved therapeutic choices. At current, an rising variety of hematologic laboratories are within the technique of integrating NGS procedures into the diagnostic algorithms of sufferers with acute myeloid leukemia (AML), myelodysplastic syndromes (MDS), and myeloproliferative neoplasms (MPNs).
Inevitably accompanying such developments, physicians and molecular biologists are dealing with sudden challenges concerning the interpretation and implementation of molecular genetic outcomes derived from NGS in myeloid malignancies. This text summarizes typical challenges (Desk 1) which will come up within the context of NGS-based analyses at analysis and through follow-up of myeloid malignancies.
Desk 1 Challenges accompanying the introduction of large parallel sequencing in scientific routine diagnostics in hemato-oncology Problem Background Present and future method Discrimination of leukemia-related mutations from polymorphisms or passenger mutations Driver mutations anticipated to happen at increased allele frequency in affected person samples than passenger mutations; driver mutations extra prone to have an effect on protein perform than polymorphisms or passenger mutations Optimization of cancer-specific databases together with reporting of uncommon physiological gene variants Implementation of novel bioinformatic algorithms primarily based on prediction of useful influence Quantitative and dynamic.
VAF monitoring (individually and along with different mutations) at follow-up Discrimination of somatic leukemia-related mutations from CHIP CHIP is offered in ~10% of people aged 70 to 80 and in as much as 20% within the age group>> 80 years Quantitative and dynamic VAF monitoring (individually and along with different mutations) at follow-up Clarifying the importance of CHIP within the context of myeloid malignancies.
Discrimination of leukemia-related somatic mutations from pathogenic germline alterations Problem to distinguish acquired somatic mutations from germline pathogenic variants at analysis Mutation detection in germline management samples (e.g., pores and skin fibroblasts, saliva) in mutations corresponding to in RUNX1, CEBPA Thorough medical household historical past adopted by molecular genetic checks in kinfolk if essential Excessive and secure VAF (e.g., 40-50%) at follow-up regardless of scientific response to therapy could also be indicative for germline alteration.
Discrimination of true genetic alterations from PCR, sequencing and post-sequencing artifacts Many artefacts are identified to come up throughout NGS library preparation, sequencing and information evaluation Error correction utilizing molecular identifiers that individually label unique enter DNA molecules Refinement of error-correction computational strategies in post-sequencing NGS information evaluation Affirmation utilizing Sanger sequencing Restricted sensitivity of NGS for minimal residual illness (MRD) evaluation Mutations detected at analysis could also be re-identified at finest to a VAF of 1-2% Error-corrected sequencing utilizing molecular identifiers.
Complementation of NGS by established MRD instruments like real-time PCR and movement cytometry Excessive monetary burden; demand on interdisciplinary approaches Costly technical and workers gear, refined information interpretation Advanced translation of NGS outcomes into therapeutic choices Growth of repeatedly up to date NGS interpretation units and algorithms for well-established mutational profiles inside distinct hematological malignancies Interdisciplinary leukemia boards VAF variant allele frequency, CHIP clonal hematopoiesis of indeterminate significance, bp base pairs, G guanine, C cytosine, ITDs inside tandem duplication.
Challenges in the introduction of next-generation sequencing (NGS) for diagnostics of myeloid malignancies into clinical routine use.

NGS-QCbox and Raspberry for Parallel, Automated and Fast High quality Management Evaluation of Massive-Scale Subsequent Technology Sequencing (Illumina) Knowledge.

Fast reputation and adaptation of subsequent technology sequencing (NGS) approaches have generated large volumes of knowledge. Excessive throughput platforms like Illumina HiSeq produce terabytes of uncooked information that requires fast processing. High quality management of the information is a crucial element previous to the downstream analyses. To deal with these points, we have now developed a high quality management pipeline, NGS-QCbox that scales as much as course of lots of or 1000’s of samples. Raspberry is an in-house device, developed in C language using HTSlib (v1.2.1) (http://htslib.org), for computing learn/base degree statistics.
It may be used as stand-alone utility and may course of each compressed and uncompressed FASTQ format recordsdata. NGS-QCbox integrates Raspberry with different open-source instruments for alignment (Bowtie2), SNP calling (SAMtools) and different utilities (bedtools) in the direction of analyzing uncooked NGS information at increased effectivity and in high-throughput method.
It stories learn and base statistics together with genome protection and variants in a consumer pleasant format. The pipeline developed presents a easy menu pushed interface and can be utilized in both fast or full mode. As well as, the pipeline in fast mode outperforms in pace in opposition to different related present QC pipeline/instruments.

Diminishing returns in next-generation sequencing (NGS) transcriptome information.

RNA-seq is more and more used to review gene expression of assorted organisms. Whereas it supplies an ideal alternative to discover genome-scale transcriptional patterns with large depth, it comes with prohibitive prices. Establishing a minimal sequencing depth for required accuracy will information cost-effective experimental design and promote the routine utility of RNA-seq. To deal with this subject, we chosen 36 RNA-seq datasets, every with greater than 20 million reads from six widely-used mannequin organisms: Saccharomyces cerevisiae, Homo sapiens, Drosophila melanogaster, Caenorhabditis elegans, Mus musculus, and Arabidopsis thaliana, and investigated statistical correlations between the sequencing depth and the result accuracy.

Diallyl disulfide

TBZ1224 ChemNorm unit Ask for price

Carbon disulfide

S-745 Scientific Laboratory Supplies 1ML 38.76 EUR

Methyl Disulfide

S-2599 Scientific Laboratory Supplies 1ML 72.96 EUR

Kinesis Hollow Cathode Lamp Tungsten 37mm Std

CHR6070 Scientific Laboratory Supplies EACH 267.9 EUR

Kinesis Hollow Cathode Lamp Tungsten 37mm unicam

CHR6071 Scientific Laboratory Supplies EACH 308.94 EUR

Kinesis Hollow Cathode Lamp Tungsten 37mm Varian

CHR6072 Scientific Laboratory Supplies EACH 308.94 EUR

Kinesis Hollow Cathode Lamp Tungsten 50mm Standard

CHR6073 Scientific Laboratory Supplies EACH 391.02 EUR

Shimadzu SPD-10AV. AVP. AVVP. 20AV Tungsten Lamp

LT-SHI-101 Scientific Laboratory Supplies EACH 225.72 EUR

ICP Std Tungsten 1000ug/mL in 2% NH4OH

PW2A7 Scientific Laboratory Supplies 100ML 177.84 EUR

ICP Std Tungsten 1000ug/mL in 2% NH4OH

PW2B7 Scientific Laboratory Supplies 250ML 287.28 EUR

ICP Std Tungsten 1000ug/mL in 2% NH4OH

PW2C7 Scientific Laboratory Supplies 500ML 392.16 EUR

ICP Std Tungsten 10000ug/mL in 2% NH4OH

PW4A7 Scientific Laboratory Supplies 100ML 310.08 EUR

ICP Std Tungsten 10000ug/mL in 2% NH4OH

PW4B7 Scientific Laboratory Supplies 250ML 370.5 EUR

Kinesis TSP uV100/1000/2000/3000 Tungsten Lamp

CHR6115 Scientific Laboratory Supplies EACH 591.66 EUR

Two (2) GAGG Sentinel Beamstop with Tungsten Housing

DBS-GAGGBS-2-B MiTeGen 2 UNITS 5358 EUR
Description: Two (2) GAGG Sentinel Beamstop with Tungsten Housing

Recombinant Disulfide Oxidoreductase

7-02692 CHI Scientific 10µg Ask for price

Recombinant Disulfide Oxidoreductase

7-02693 CHI Scientific 50µg Ask for price

Recombinant Disulfide Oxidoreductase

7-02694 CHI Scientific 1mg Ask for price

Disulfide Oxidoreductase (Recombinant)

20-abx073036 Abbexa
  • 276.00 EUR
  • 2214.00 EUR
  • 393.60 EUR
  • 10 ug
  • 1 mg
  • 50 ug

Formamidine disulfide dihydrochloride

GK5373-25G Glentham Life Sciences 25 g 160.8 EUR

LKB Pharmacia Ultrospec 2000 3000 Novaspec ii Tungsten Lamp

LT-LKB-100 Scientific Laboratory Supplies EACH 39.9 EUR

Unicam 5625 5675 8600 8700 8800 Series Tungsten Lamp

LT-PHU-102 Scientific Laboratory Supplies EACH 145.92 EUR

Kinesis Hollow Cathode Lamp Tungsten 50mm PE AAnalyst (Lumina)

CHR6074 Scientific Laboratory Supplies EACH 494.76 EUR

Recombinant Disulfide Bond Isomerase

7-02695 CHI Scientific 10µg Ask for price

Recombinant Disulfide Bond Isomerase

7-02696 CHI Scientific 50µg Ask for price

Recombinant Disulfide Bond Isomerase

7-02697 CHI Scientific 1mg Ask for price

Protein disulfide-isomerase Antibody

20-abx110136 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase Antibody

20-abx110137 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Disulfide Isomerase Protein (Recombinant)

20-abx073027 Abbexa
  • 1128.00 EUR
  • 444.00 EUR
  • 1713.60 EUR
  • 0.5 mg
  • 100 ug
  • 1 mg

Disulfide Bond Isomerase (Recombinant)

20-abx073037 Abbexa
  • 276.00 EUR
  • 2214.00 EUR
  • 393.60 EUR
  • 10 ug
  • 1 mg
  • 50 ug

N1-Glutathionyl-spermidine disulfide

H-1126.0005 Bachem 5.0mg 544.8 EUR
Description: Sum Formula: C34H66N12O10S2; CAS# [108081-77-2]

N1-Glutathionyl-spermidine disulfide

H-1126.0025 Bachem 25.0mg 2067.6 EUR
Description: Sum Formula: C34H66N12O10S2; CAS# [108081-77-2]

Recombinant (E.Coli) Disulfide Oxidoreductase

RP-365 Alpha Diagnostics 10 ug 270 EUR

Cecil Series 2. 1000 to 9000. CE Sereis Tungsten Lamp

LT-CEC-111 Scientific Laboratory Supplies EACH 87.78 EUR

Unicam UV1 Helios Alpha. Beta Gamma and Delta Tungsten Lamp

LT-PHU-103 Scientific Laboratory Supplies EACH 112.86 EUR

ICP Std Tungsten 1000ug/mL in 1% HNO3 + 2% HF

PW2A14 Scientific Laboratory Supplies 100ML 177.84 EUR

ICP Std Tungsten 1000ug/mL in 1% HNO3 + 2% HF

PW2B14 Scientific Laboratory Supplies 250ML 525.54 EUR

ICP Std Tungsten 10000ug/mL in 1% HNO3 + 2% HF

PW4B15 Scientific Laboratory Supplies 250ML 388.74 EUR

Human Protein disulfide-isomerase (P4HB)

1-CSB-RP002544h Cusabio
  • 456.00 EUR
  • 256.80 EUR
  • 1570.80 EUR
  • 672.00 EUR
  • 1047.60 EUR
  • 314.40 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Protein disulfide-isomerase(P4HB),partial expressed in E.coli

Human Protein disulfide-isomerase (P4hb)

1-CSB-YP017342HU Cusabio
  • 516.00 EUR
  • 280.80 EUR
  • 1809.60 EUR
  • 770.40 EUR
  • 1210.80 EUR
  • 349.20 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Protein disulfide-isomerase(P4hb) expressed in Yeast

Human Protein disulfide-isomerase (P4HB)

1-CSB-YP017342HUf8 Cusabio
  • 516.00 EUR
  • 280.80 EUR
  • 1809.60 EUR
  • 770.40 EUR
  • 1210.80 EUR
  • 349.20 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Protein disulfide-isomerase(P4HB),partial expressed in Yeast

Human Protein disulfide-isomerase (P4HB)

1-CSB-EP017342HU Cusabio
  • 456.00 EUR
  • 256.80 EUR
  • 1570.80 EUR
  • 672.00 EUR
  • 1047.60 EUR
  • 314.40 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Protein disulfide-isomerase(P4HB) expressed in E.coli

PDI (Protein Disulfide Isomerase) Antibody

5601-100 Biovision 379.2 EUR

PDI (Protein Disulfide Isomerase) Antibody

5601-30T Biovision 175.2 EUR

Recombinant Human Protein Disulfide Isomerase

7-03304 CHI Scientific 100µg Ask for price

Recombinant Human Protein Disulfide Isomerase

7-03305 CHI Scientific 500µg Ask for price

Recombinant Human Protein Disulfide Isomerase

7-03306 CHI Scientific 1mg Ask for price

Protein disulfide-isomerase Polyclonal Antibody

42079-100ul SAB 100ul 399.6 EUR

Protein Disulfide Isomerase (PDI) Antibody

20-abx102689 Abbexa
  • 510.00 EUR
  • 159.60 EUR
  • 1412.40 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide Isomerase (PDI) Antibody

20-abx102690 Abbexa
  • 526.80 EUR
  • 159.60 EUR
  • 1496.40 EUR
  • 710.40 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein disulfide-isomerase Antibody (HRP)

20-abx108658 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase Antibody (HRP)

20-abx108659 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase TMX3 Antibody

20-abx110139 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase Antibody (Biotin)

20-abx105821 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase Antibody (Biotin)

20-abx105825 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase Antibody (FITC)

20-abx107238 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase Antibody (FITC)

20-abx107239 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Disulfide Isomerase A3 Protein (Recombinant)

20-abx073097 Abbexa
  • 3433.20 EUR
  • 393.60 EUR
  • 276.00 EUR
  • 1 mg
  • 25 ug
  • 5 ug

Disulfide Isomerase A6 Protein (Recombinant)

20-abx073169 Abbexa
  • 4101.60 EUR
  • 393.60 EUR
  • 276.00 EUR
  • 1 mg
  • 20 ug
  • 5 ug

Disulfide Isomerase A4 Protein (Recombinant)

20-abx073751 Abbexa
  • 393.60 EUR
  • 7676.40 EUR
  • 276.00 EUR
  • 10 ug
  • 1 mg
  • 2 µg

Protein Disulfide Isomerase (PDI) Antibody

20-abx174245 Abbexa
  • 978.00 EUR
  • 510.00 EUR
  • 1 mg
  • 200 ug

Protein Disulfide-Isomerase (P4HB) Antibody

abx145769-100ug Abbexa 100 ug 469.2 EUR

Protein Disulfide Isomerase (PDI) Antibody

20-abx141570 Abbexa
  • 444.00 EUR
  • 727.20 EUR
  • 360.00 EUR
  • 100 ul
  • 200 ul
  • 50 ul

Protein Disulfide-Isomerase (P4HB) Antibody

20-abx126992 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 100 ul
  • 200 ul

Protein Disulfide Isomerase (PDI) Antibody

20-abx128556 Abbexa
  • 493.20 EUR
  • 159.60 EUR
  • 1378.80 EUR
  • 678.00 EUR
  • 376.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide-Isomerase (P4HB) Antibody

20-abx000827 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 218.40 EUR
  • 376.80 EUR
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Protein Disulfide-Isomerase (P4HB) Antibody

abx031784-400ul Abbexa 400 ul 627.6 EUR

Protein Disulfide-Isomerase (P4HB) Antibody

abx031784-80l Abbexa 80 µl 343.2 EUR

Protein Disulfide Isomerase (P4HB) Antibody

20-abx445165 Abbexa
  • 627.60 EUR
  • 393.60 EUR
  • 100 ul
  • 25 ul

Protein Disulfide-Isomerase (P4HB) Antibody

20-abx300560 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein Disulfide Isomerase (PDI) Antibody

abx236272-100ug Abbexa 100 ug 577.2 EUR

DsbA Disulfide Oxidoreductase Recombinant Protein

PROTP0AEG4 BosterBio Regular: 50ug 380.4 EUR
Description: Disulfide Oxidoreductase produced in E.Coli is a periplasmic protein isolated from E. coli, containing 208 amino acids having a molecular mass of 23,149 Dalton. ;The DsbA is purified by proprietary chromatographic techniques.

PDI, Protein Disulfide Isomerase, human

RC512-13 Bio Basic 100ug 307.96 EUR

Recombinant Protein Disulfide Isomerase (PDI)

4-RPB061Hu01 Cloud-Clone
  • 582.34 EUR
  • 279.60 EUR
  • 1853.76 EUR
  • 697.92 EUR
  • 1275.84 EUR
  • 465.60 EUR
  • 4454.40 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Protein Disulfide Isomerase expressed in: E.coli

Recombinant Protein Disulfide Isomerase (PDI)

4-RPB061Mu01 Cloud-Clone
  • 582.34 EUR
  • 279.60 EUR
  • 1853.76 EUR
  • 697.92 EUR
  • 1275.84 EUR
  • 465.60 EUR
  • 4454.40 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Protein Disulfide Isomerase expressed in: E.coli

Recombinant Protein Disulfide Isomerase (PDI)

4-RPB061Ra01 Cloud-Clone
  • 631.80 EUR
  • 292.80 EUR
  • 2039.23 EUR
  • 759.74 EUR
  • 1399.49 EUR
  • 498.00 EUR
  • 4918.08 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Protein Disulfide Isomerase expressed in: E.coli

Recombinant (E.Coli) Disulfide Bond Isomerase

RP-378 Alpha Diagnostics 10 ug 270 EUR

Cricetulus griseus Protein disulfide-isomerase (P4HB)

1-CSB-EP823188DXU Cusabio
  • 733.20 EUR
  • 370.80 EUR
  • 2192.40 EUR
  • 1126.80 EUR
  • 1461.60 EUR
  • 476.40 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Cricetulus griseus Protein disulfide-isomerase(P4HB) expressed in E.coli

Human Protein disulfide-isomerase TMX3 (TMX3)

1-CSB-EP846645HU Cusabio
  • 456.00 EUR
  • 256.80 EUR
  • 1570.80 EUR
  • 672.00 EUR
  • 1047.60 EUR
  • 314.40 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Protein disulfide-isomerase TMX3(TMX3) expressed in E.coli

Human Protein Disulfide Isomerase ELISA kit

E01P0078-192T BlueGene 192 tests 1524 EUR
Description: A competitive ELISA for quantitative measurement of Human Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Protein Disulfide Isomerase ELISA kit

E01P0078-48 BlueGene 1 plate of 48 wells 624 EUR
Description: A competitive ELISA for quantitative measurement of Human Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Protein Disulfide Isomerase ELISA kit

E01P0078-96 BlueGene 1 plate of 96 wells 822 EUR
Description: A competitive ELISA for quantitative measurement of Human Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Protein disulfide-isomerase A2 (Pdia2)

1-CSB-YP017719MO Cusabio
  • 604.80 EUR
  • 318.00 EUR
  • 2198.40 EUR
  • 915.60 EUR
  • 1459.20 EUR
  • 400.80 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Protein disulfide-isomerase A2(Pdia2) expressed in Yeast

Cricetulus griseus Protein disulfide-isomerase (P4HB)

1-CSB-YP823188DXU Cusabio
  • 814.80 EUR
  • 402.00 EUR
  • 2606.40 EUR
  • 1261.20 EUR
  • 1730.40 EUR
  • 522.00 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Cricetulus griseus Protein disulfide-isomerase(P4HB) expressed in Yeast

Anti-MSLN-disulfide linker-DM4 ADC

ADC-W-426 Creative Biolabs 1mg Ask for price
Description: This ADC product is comprised of an anti-MSLN monoclonal antibody conjugated via a disulfide linker to a DM4

Goat Protein Disulfide Isomerase ELISA kit

E06P0078-192T BlueGene 192 tests 1524 EUR
Description: A competitive ELISA for quantitative measurement of Goat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Protein Disulfide Isomerase ELISA kit

E06P0078-48 BlueGene 1 plate of 48 wells 624 EUR
Description: A competitive ELISA for quantitative measurement of Goat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Protein Disulfide Isomerase ELISA kit

E06P0078-96 BlueGene 1 plate of 96 wells 822 EUR
Description: A competitive ELISA for quantitative measurement of Goat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Protein Disulfide Isomerase ELISA kit

E08P0078-192T BlueGene 192 tests 1524 EUR
Description: A competitive ELISA for quantitative measurement of Canine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Protein Disulfide Isomerase ELISA kit

E08P0078-48 BlueGene 1 plate of 48 wells 624 EUR
Description: A competitive ELISA for quantitative measurement of Canine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Protein Disulfide Isomerase ELISA kit

E08P0078-96 BlueGene 1 plate of 96 wells 822 EUR
Description: A competitive ELISA for quantitative measurement of Canine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Protein Disulfide Isomerase ELISA kit

E04P0078-192T BlueGene 192 tests 1524 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Protein Disulfide Isomerase ELISA kit

E04P0078-48 BlueGene 1 plate of 48 wells 624 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Protein Disulfide Isomerase ELISA kit

E04P0078-96 BlueGene 1 plate of 96 wells 822 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Protein Disulfide Isomerase ELISA kit

E07P0078-192T BlueGene 192 tests 1524 EUR
Description: A competitive ELISA for quantitative measurement of Porcine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Protein Disulfide Isomerase ELISA kit

E07P0078-48 BlueGene 1 plate of 48 wells 624 EUR
Description: A competitive ELISA for quantitative measurement of Porcine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Protein Disulfide Isomerase ELISA kit

E07P0078-96 BlueGene 1 plate of 96 wells 822 EUR
Description: A competitive ELISA for quantitative measurement of Porcine Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Protein Disulfide Isomerase ELISA kit

E03P0078-192T BlueGene 192 tests 1524 EUR
Description: A competitive ELISA for quantitative measurement of Mouse Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Protein Disulfide Isomerase ELISA kit

E03P0078-48 BlueGene 1 plate of 48 wells 624 EUR
Description: A competitive ELISA for quantitative measurement of Mouse Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Protein Disulfide Isomerase ELISA kit

E03P0078-96 BlueGene 1 plate of 96 wells 822 EUR
Description: A competitive ELISA for quantitative measurement of Mouse Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Protein Disulfide Isomerase ELISA kit

E02P0078-192T BlueGene 192 tests 1524 EUR
Description: A competitive ELISA for quantitative measurement of Rat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Protein Disulfide Isomerase ELISA kit

E02P0078-48 BlueGene 1 plate of 48 wells 624 EUR
Description: A competitive ELISA for quantitative measurement of Rat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Protein Disulfide Isomerase ELISA kit

E02P0078-96 BlueGene 1 plate of 96 wells 822 EUR
Description: A competitive ELISA for quantitative measurement of Rat Protein Disulfide Isomerase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Protein disulfide-isomerase Polyclonal Conjugated Antibody

C42079 SAB 100ul 476.4 EUR

Mouse Protein disulfide-isomerase A2 (Pdia2)

1-CSB-EP017719MO Cusabio
  • 606.00 EUR
  • 318.00 EUR
  • 2192.40 EUR
  • 919.20 EUR
  • 1461.60 EUR
  • 402.00 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Protein disulfide-isomerase A2(Pdia2) expressed in E.coli

Human Protein disulfide-isomerase A3 (PDIA3)

1-CSB-EP017720HU Cusabio
  • 606.00 EUR
  • 318.00 EUR
  • 2192.40 EUR
  • 919.20 EUR
  • 1461.60 EUR
  • 402.00 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Protein disulfide-isomerase A3(PDIA3) expressed in E.coli

Recombinant E.Coli Thiol Disulfide Interchange Protein

7-06964 CHI Scientific 5µg Ask for price

Recombinant E.Coli Thiol Disulfide Interchange Protein

7-06965 CHI Scientific 25µg Ask for price

Recombinant E.Coli Thiol Disulfide Interchange Protein

7-06966 CHI Scientific 1mg Ask for price

Recombinant E.Coli Thiol Disulfide Interchange Protein

7-06967 CHI Scientific 5µg Ask for price

Recombinant E.Coli Thiol Disulfide Interchange Protein

7-06968 CHI Scientific 25µg Ask for price

Recombinant E.Coli Thiol Disulfide Interchange Protein

7-06969 CHI Scientific 1mg Ask for price

Recombinant Human Protein Disulfide Isomerase A3

7-03307 CHI Scientific 5µg Ask for price

Recombinant Human Protein Disulfide Isomerase A3

7-03308 CHI Scientific 25µg Ask for price

Recombinant Human Protein Disulfide Isomerase A3

7-03309 CHI Scientific 1mg Ask for price

Recombinant Human Protein Disulfide Isomerase A4

7-03310 CHI Scientific 2µg Ask for price

Recombinant Human Protein Disulfide Isomerase A4

7-03311 CHI Scientific 10µg Ask for price

Recombinant Human Protein Disulfide Isomerase A4

7-03312 CHI Scientific 1mg Ask for price

Recombinant Human Protein Disulfide Isomerase A6

7-03313 CHI Scientific 5µg Ask for price

Recombinant Human Protein Disulfide Isomerase A6

7-03314 CHI Scientific 20µg Ask for price

Recombinant Human Protein Disulfide Isomerase A6

7-03315 CHI Scientific 1mg Ask for price

Protein Disulfide-Isomerase A6 Recombinant Protein

91-476 ProSci 0.05 mg 651.3 EUR
Description: Protein Disulfide-Isomerase A6 (PDIA6) is a 48.5kDa protein that belongs to the protein disulfide isomerase family (PDI). PDIA6 is an enzyme in the endoplasmic reticulum in eukaryotes which catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. The PDIA6 expressed in platelets, its functions as a chaperone that inhibits aggregation of misfolded proteins. PDIA6 is part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1. PDIA6 also plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.

Protein Disulfide-Isomerase A4 Recombinant Protein

91-762 ProSci 0.05 mg 651.3 EUR
Description: Protein Disulfide-Isomerase A4 (PDIA4) is an endoplasmic reticulum luminal protein that belongs to the protein disulfide isomerase family. Human PDIA4 is synthesized as a 625 amino acid precursor that contains a 20 amino acid signal sequence, and a 625 amino acid mature chain, including three thioredoxin domains. PDIA4 catalyzes the rearrangement of -S-S- bonds in proteins and is thought to be a deoxycytidine kinase. In addition, PDIA4 serves as a proteases protein disulfide isomerase, phospholipase or an arrangement of these.

Protein disulfide-isomerase A3 Recombinant Protein

91-869 ProSci 0.05 mg 852.9 EUR
Description: PDIA3 protein is also known as Protein disulfide-isomerase A3. It is a protein that in humans is encoded by the PDIA3 gene.PDIA3 is an enzyme that belongs to the endoplasmic reticulum and interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. PDIA3 interacts with thiazide-sensitive sodium-chloride cotransporter in the kidney and is induced by glucose deprivation. PDIA3 is part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is important for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface.

Protein disulfide-isomerase A5 Recombinant Protein

91-965 ProSci 0.05 mg 852.9 EUR
Description: Protein disulfide-isomerase A5 is a 519 amino acids protein that belongs to the protein disulfide isomerase family.It contains 3 thioredoxin domains.It can catalyze the rearrangement of -S-S- bonds in proteins.

Protein disulfide-isomerase A2 Antibody (HRP)

20-abx108660 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase A3 Antibody (HRP)

20-abx108661 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase TMX3 Antibody (HRP)

20-abx108662 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein Disulfide-Isomerase A2 (Pdia2) Antibody

20-abx109353 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein Disulfide-Isomerase A2 (Pdia3) Antibody

20-abx110138 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase A2 Antibody (Biotin)

20-abx105822 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase A3 Antibody (Biotin)

20-abx105823 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase TMX3 Antibody (Biotin)

20-abx105824 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase A2 Antibody (FITC)

20-abx107240 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase A3 Antibody (FITC)

20-abx107241 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein disulfide-isomerase TMX3 Antibody (FITC)

20-abx107242 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Protein Disulfide Isomerase (PDI) Protein

20-abx068737 Abbexa
  • 811.20 EUR
  • 343.20 EUR
  • 2498.40 EUR
  • 961.20 EUR
  • 577.20 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Protein Disulfide Isomerase (PDI) Protein

20-abx068738 Abbexa
  • 878.40 EUR
  • 343.20 EUR
  • 2749.20 EUR
  • 1045.20 EUR
  • 627.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Protein Disulfide Isomerase (PDI) Protein

20-abx068739 Abbexa
  • 811.20 EUR
  • 343.20 EUR
  • 2498.40 EUR
  • 961.20 EUR
  • 577.20 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide Isomerase A2 (PDIA2) Antibody

20-abx103307 Abbexa
  • 510.00 EUR
  • 159.60 EUR
  • 1412.40 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide Isomerase A3 (PDIA3) Antibody

20-abx103308 Abbexa
  • 493.20 EUR
  • 159.60 EUR
  • 1378.80 EUR
  • 678.00 EUR
  • 376.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide Isomerase A2 (PDIA2) Antibody

20-abx174246 Abbexa
  • 978.00 EUR
  • 510.00 EUR
  • 1 mg
  • 200 ug

Protein Disulfide Isomerase A3 (PDIA3) Antibody

20-abx174247 Abbexa
  • 978.00 EUR
  • 510.00 EUR
  • 1 mg
  • 200 ug

Protein Disulfide Isomerase A4 (PDIA4) Antibody

20-abx174248 Abbexa
  • 1028.40 EUR
  • 526.80 EUR
  • 1 mg
  • 200 ug

Protein Disulfide Isomerase A5 (PDIA5) Antibody

20-abx174249 Abbexa
  • 1028.40 EUR
  • 526.80 EUR
  • 1 mg
  • 200 ug

Protein Disulfide Isomerase A6 (PDIA6) Antibody

20-abx174250 Abbexa
  • 1028.40 EUR
  • 526.80 EUR
  • 1 mg
  • 200 ug

Protein Disulfide-Isomerase (P4HB) Antibody Pair

abx117373-1pair5x96wellplates Abbexa 1 pair (5x96 well plates) 1212 EUR

Protein Disulfide Isomerase A6 (PDIA6) Antibody

20-abx131442 Abbexa
  • 510.00 EUR
  • 159.60 EUR
  • 1446.00 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide Isomerase A3 (PDIA3) Antibody

20-abx127001 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 100 ul
  • 200 ul

Protein Disulfide Isomerase A5 (PDIA5) Antibody

20-abx128192 Abbexa
  • 510.00 EUR
  • 159.60 EUR
  • 1446.00 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide Isomerase A4 (PDIA4) Antibody

20-abx128208 Abbexa
  • 510.00 EUR
  • 159.60 EUR
  • 1446.00 EUR
  • 693.60 EUR
  • 393.60 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide Isomerase A2 (PDIA2) Antibody

20-abx128942 Abbexa
  • 493.20 EUR
  • 159.60 EUR
  • 1378.80 EUR
  • 678.00 EUR
  • 376.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Disulfide Isomerase A6 (PDIA6) Antibody

20-abx005339 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 218.40 EUR
  • 376.80 EUR
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Protein Disulfide Isomerase A3 (PDIA3) Antibody

20-abx001008 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 218.40 EUR
  • 376.80 EUR
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Protein Disulfide Isomerase A6 (PDIA6) Antibody

20-abx002406 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 100 ul
  • 200 ul

Protein Disulfide-Isomerase A4 (PDIA4) Antibody

20-abx003217 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 100 ul
  • 200 ul

Protein Disulfide Isomerase A5 (PDIA5) Antibody

abx027813-400ul Abbexa 400 ul 627.6 EUR

Protein Disulfide Isomerase A5 (PDIA5) Antibody

abx027813-80l Abbexa 80 µl 343.2 EUR

Protein Disulfide-Isomerase A4 (PDIA4) Antibody

abx027892-400ul Abbexa 400 ul 627.6 EUR

Protein Disulfide-Isomerase A4 (PDIA4) Antibody

abx027892-80l Abbexa 80 µl 343.2 EUR

Protein Disulfide Isomerase A6 (PDIA6) Antibody

abx032922-400ul Abbexa 400 ul 627.6 EUR

Protein Disulfide Isomerase A6 (PDIA6) Antibody

abx032922-80l Abbexa 80 µl 343.2 EUR

Protein Disulfide Isomerase A6 (PDIA6) Antibody

abx032923-400ul Abbexa 400 ul 627.6 EUR

Protein Disulfide Isomerase A6 (PDIA6) Antibody

abx032923-80l Abbexa 80 µl 343.2 EUR

Protein Disulfide Isomerase A3 (PDIA3) Antibody

abx031794-400ul Abbexa 400 ul 627.6 EUR

Protein Disulfide Isomerase A3 (PDIA3) Antibody

abx031794-80l Abbexa 80 µl 343.2 EUR
To realize this, we randomly selected reads from every dataset, mapped them to the reference genomes, and analyzed the accuracy achieved with various protection. Our outcomes indicated that as little as a million reads can present the identical sequencing accuracy in transcript abundance (r=0.99) as >30 million reads for highly-expressed genes in all six species. As a result of many metabolically and pathologically-relevant genes are extremely expressed, our findings is perhaps instructive for cost-effective experimental designs in NGS-based analysis and likewise present helpful steerage to related analysis for different organisms.