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Rapid identification of fruit length loci in cucumber (Cucumis sativus L.) using next-generation sequencing (NGS)-based QTL analysis.

The cucumber (Cucumis sativus L.) reveals intensive variations in fruit measurement and form. Fruit size is a crucial agronomic and domesticated trait managed by quantitative trait loci (QTLs). Nonetheless, the underlying molecular and genetic mechanisms that decide cucumber fruit size stay unclear. QTL-seq is an environment friendly technique for QTL identification that takes benefit of bulked-segregant evaluation (BSA) and next-generation sequencing (NGS).
Within the current examine, we performed QTL mapping and QTL-seq of cucumber fruit size. QTL mapping recognized eight QTLs for immature and mature fruit size. A significant-effect QTL fl3.2, which defined a most of 38.87% of the phenotypic variation, was detected. A genome-wide comparability of SNP profiles between two DNA bulks recognized 6 QTLs for ovary size. QTLs ovl3.1 and ovl3.2 each had main results on ovary size with a △ (SNP-index) of 0.80 (P < 0.01) and 0.74 (P < 0.01), respectively.
Quantitative RT-PCR of fruit size-related homologous genes localized within the consensus QTL FL3.2 was performed. 4 candidate genes exhibited elevated expression ranges in lengthy fruit genotypes. Our outcomes demonstrated the ability of the QTL-seq methodology in speedy QTL detection and supplied dependable QTL areas for fantastic mapping of fruit length-related loci and for figuring out candidate genes.
Dependable multiplex sequencing with uncommon index mis-assignment on DNB-based NGS platform.
Massively-parallel-sequencing, coupled with pattern multiplexing, has made genetic exams broadly inexpensive. Nonetheless, intractable index mis-assignments (generally exceeds 1%) have been repeatedly reported on some extensively used sequencing platforms.Right here, we investigated this high quality subject on BGI sequencers utilizing three library preparation strategies: complete genome sequencing (WGS) with PCR, PCR-free WGS, and two-step focused PCR.

BGI’s sequencers make the most of a novel DNA nanoball (DNB) expertise which makes use of rolling circle replication for DNA-nanoball preparation; this linear amplification is PCR free and might keep away from error accumulation. We demonstrated that single index mis-assignment from free listed oligos happens at a fee of 1 in 36 million reads, suggesting just about no index hopping throughout DNB creation and arraying.
Moreover, the DNB-based NGS libraries have achieved an unprecedentedly low sample-to-sample mis-assignment fee of 0.0001 to 0.0004% underneath really helpful procedures.Single indexing with DNB expertise supplies a easy however efficient methodology for delicate genetic assays with giant pattern numbers.
Subsequent-generation sequencing of immunoglobulin gene rearrangements for clonality evaluation: a technical feasibility examine by EuroClonality-NGS.
One of many hallmarks of B lymphoid malignancies is a B cell clone characterised by a novel footprint of clonal immunoglobulin (IG) gene rearrangements that serves as a diagnostic marker for clonality evaluation. The EuroClonality/BIOMED-2 assay is presently the gold customary for analyzing IG heavy chain (IGH) and κ mild chain (IGK) gene rearrangements of suspected B cell lymphomas.
Right here, the EuroClonality-NGS Working Group presents a multicentre technical feasibility examine of a novel method involving next-generation sequencing (NGS) of IGH and IGK loci rearrangements that’s extremely appropriate for detecting IG gene rearrangements in frozen and formalin-fixed paraffin-embedded tissue specimens.
By using gene-specific primers for IGH and IGK amplifying smaller amplicon sizes together with deep sequencing expertise, this NGS-based IG clonality evaluation confirmed strong efficiency, even in DNA samples of suboptimal DNA integrity, and a excessive medical sensitivity for the detection of clonal rearrangements.
Bioinformatics analyses of the high-throughput sequencing knowledge with ARResT/Interrogate, a platform developed inside the EuroClonality-NGS Working Group, allowed correct identification of clonotypes in each polyclonal cell populations and monoclonal lymphoproliferative problems. This multicentre feasibility examine is a crucial step in direction of implementation of NGS-based clonality evaluation in medical observe, which is able to finally enhance lymphoma diagnostics.
Excessive-throughput evaluation of the actions of xCas9, SpCas9-NG and SpCas9 at matched and mismatched goal sequences in human cells.
The functions of clustered commonly interspaced quick palindromic repeats (CRISPR)-based genome modifying may be restricted by a scarcity of appropriate protospacer adjoining motifs (PAMs), inadequate on-target exercise and off-target results. Right here, we report an in depth comparability of the PAM-sequence compatibilities and the on-target and off-target actions of Cas9 from Streptococcus pyogenes (SpCas9) and the SpCas9 variants xCas9 and SpCas9-NG (that are identified to have broader PAM compatibility than SpCas9) at 26,478 lentivirally built-in goal sequences and 78 endogenous goal websites in human cells.
We discovered that xCas9 has the bottom tolerance for mismatched goal sequences and that SpCas9-NG has the broadest PAM compatibility. We additionally present, on the premise of newly recognized non-NGG PAM sequences, that SpCas9-NG and SpCas9 can edit six beforehand unedited endogenous websites related to genetic illnesses.
Picric Acid extrapure AR, 99.8% |
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47998 | Sisco Laboratories | 100 Gms | 3.01 EUR |
Description: Part A |
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Benzoic Acid extrapure AR, 99.5% |
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64239 | Sisco Laboratories | 500 Gms | 4.93 EUR |
Description: Part A |
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Malonic Acid extrapure AR, 99% |
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26504 | Sisco Laboratories | 100 Gms | 2.48 EUR |
Description: Part B |
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Fumaric Acid extrapure AR, 99% |
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79503 | Sisco Laboratories | 500 Gms | 4.11 EUR |
Description: Part A |
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Molybdic Acid extrapure AR, 85% |
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98466 | Sisco Laboratories | 100 Gms | 11.97 EUR |
Description: Part A |
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Succinic Acid extrapure AR, 99.5% |
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85752 | Sisco Laboratories | 100 Gms | 2.87 EUR |
Description: Part A |
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Tungstic Acid extrapure AR, 99% |
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55401 | Sisco Laboratories | 100 Gms | 10.13 EUR |
Description: Part B |
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Calconcarboxylic Acid extrapure AR |
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65326 | Sisco Laboratories | 5 Gms | 1.5 EUR |
Description: Part B |
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Salicylic Acid extrapure AR, 99.9% |
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42568 | Sisco Laboratories | 500 Gms | 5.13 EUR |
Description: Part A |
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Sulphamic Acid extrapure AR, 99.5% |
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42773 | Sisco Laboratories | 100 Gms | 2.26 EUR |
Description: Part A |
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Barbituric Acid extrapure AR, 99% |
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21154 | Sisco Laboratories | 25 Gms | 2.33 EUR |
Description: Part A |
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EDTA Free Acid extrapure AR, 99% |
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18240 | Sisco Laboratories | 100 Gms | 1.71 EUR |
Description: Part A |
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L-Lactic Acid extrapure AR, 88% |
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41242 | Sisco Laboratories | 500 ml | 4.79 EUR |
Description: Part A |
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L-Ascorbic Acid extrapure AR, 99.7% |
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23006 | Sisco Laboratories | 100 Gms | 1.37 EUR |
Description: Part A |
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Salicylic Acid extrapure AR, ACS, 99.9% |
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62657 | Sisco Laboratories | 500 Gms | 5.82 EUR |
Description: Part A |
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m-Aminobenzoic Acid extrapure AR, 99% |
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74004 | Sisco Laboratories | 25 Gms | 6 EUR |
Description: Part B |
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m-Nitrobenzoic Acid extrapure AR, 99% |
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81431 | Sisco Laboratories | 25 Gms | 3.23 EUR |
Description: Part B |
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p-Chlorobenzoic Acid extrapure AR, 99.7% |
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97199 | Sisco Laboratories | 100 Gms | 8.33 EUR |
Description: Part B |
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o-Chlorobenzoic Acid extrapure AR, 99.5% |
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30519 | Sisco Laboratories | 100 Gms | 3.68 EUR |
Description: Part B |
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3,5-Dinitrobenzoic Acid extrapure AR, 99% |
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66764 | Sisco Laboratories | 100 Gms | 5.4 EUR |
Description: Part B |
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Orthophosphoric Acid extrapure AR, ACS, 85% |
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50056 | Sisco Laboratories | 500 ml | 4.45 EUR |
Description: Part A |
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Trichloroacetic Acid extrapure AR, ACS, 99.5% |
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60677 | Sisco Laboratories | 100 Gms | 1.98 EUR |
Description: Part A |
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Phosphomolybdic Acid extrapure AR, ACS, 99% |
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82322 | Sisco Laboratories | 25 Gms | 8.21 EUR |
Description: Part A |
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3,5-Dinitrosalicylic Acid extrapure AR, 99% |
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38707 | Sisco Laboratories | 25 Gms | 10.35 EUR |
Description: Part B |
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Myristic Acid extrapure AR, C14-99% (GC) |
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36459 | Sisco Laboratories | 100 Gms | 1.8 EUR |
Description: Part B |
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Propionic Acid extrapure AR, C3-99.5% (GC) |
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43883 | Sisco Laboratories | 500 ml | 4.38 EUR |
Description: Part A |
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Acetic Acid Glacial extrapure AR, 99.9% |
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85801 | Sisco Laboratories | 500 ml | 1.78 EUR |
Description: Part A |
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Tungstosilicic Acid Hydrate extrapure AR |
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71290 | Sisco Laboratories | 25 Gms | 7.39 EUR |
Description: Part A |
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Dithiooxamide (Rubeanic Acid) extrapure AR, 99% |
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48086 | Sisco Laboratories | 1 Gms | 8.93 EUR |
Description: Part B |
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Phosphotungstic Acid Hydrate extrapure AR |
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57855 | Sisco Laboratories | 25 Gms | 6.16 EUR |
Description: Part A |
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n-Butyric Acid extrapure AR, C4-99.5% (GC) |
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55964 | Sisco Laboratories | 250 ml | 2.19 EUR |
Description: Part A |
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Trifluoroacetic Acid (TFA) extrapure AR, 99.5% |
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65415 | Sisco Laboratories | 100 ml | 16.56 EUR |
Description: Part A |
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Hypophosphorous Acid extrapure AR, 50% in water |
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92379 | Sisco Laboratories | 500 ml | 20.53 EUR |
Description: Part A |
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2-Thiobarbituric Acid (TBA) extrapure AR, 99% |
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75241 | Sisco Laboratories | 25 Gms | 7.53 EUR |
Description: Part A |
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Sulphanilic Acid (High Purity) extrapure AR, 99.5% |
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93360 | Sisco Laboratories | 100 Gms | 2.26 EUR |
Description: Part A |
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2,3,5-Triiodobenzoic Acid (TIBA) extrapure AR, 98% |
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37758 | Sisco Laboratories | 5 Gms | 6.75 EUR |
Description: Part B |
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Nitrilotriacetic Acid (NTA) extrapure AR, ACS, 99% |
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48081 | Sisco Laboratories | 100 Gms | 2.05 EUR |
Description: Part A |
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N-Phenylanthranilic Acid (2-Anilinobenzoic Acid) extrapure AR, 99% |
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14059 | Sisco Laboratories | 25 Gms | 10.73 EUR |
Description: Part B |
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Maleic Acid extrapure AR, ExiPlus, Multi-Compendial, 99.5% |
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68589 | Sisco Laboratories | 100 Gms | 1.71 EUR |
Description: Part A |
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Fumaric Acid extrapure AR, ExiPlus, Multi-Compendial, 99% |
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97328 | Sisco Laboratories | 500 Gms | 5.47 EUR |
Description: Part A |
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Tannic Acid extrapure AR, ACS, ExiPlus, Multi-Compendial |
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92101 | Sisco Laboratories | 100 Gms | 8.28 EUR |
Description: Part A |
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Cinnamic Acid extrapure AR, ExiPlus, Multi-Compendial, 99% |
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23277 | Sisco Laboratories | 250 Gms | 9.58 EUR |
Description: Part A |
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Diethylenetriaminepentaacetic Acid (DTPA) extrapure AR, 99% |
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64699 | Sisco Laboratories | 100 Gms | 3.68 EUR |
Description: Part B |
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a-Ketoglutaric Acid (High Purity) extrapure AR, 99.5% |
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28386 | Sisco Laboratories | 25 Gms | 5.78 EUR |
Description: Part B |
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Calconcarboxylic Acid extrapure AR, ExiPlus, Multi-Compendial |
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66406 | Sisco Laboratories | 25 Gms | 6.6 EUR |
Description: Part B |
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Boric Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
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61871 | Sisco Laboratories | 500 Gms | 3.76 EUR |
Description: Part A |
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Arsenazo III Free Acid (High Purity) extrapure AR, 85% |
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47014 | Sisco Laboratories | 1 Gms | 56.25 EUR |
Description: Part B |
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Benzoic Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
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10105 | Sisco Laboratories | 500 Gms | 9.58 EUR |
Description: Part A |
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Succinic Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
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62321 | Sisco Laboratories | 100 Gms | 3.15 EUR |
Description: Part A |
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Molybdic Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 85% |
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71772 | Sisco Laboratories | 100 Gms | 13.82 EUR |
Description: Part A |
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Sulphamic Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
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81645 | Sisco Laboratories | 100 Gms | 2.94 EUR |
Description: Part A |
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1-Amino-2-Naphthol-4-Sulphonic Acid (1,2,4-Acid) extrapure AR, ACS, 98% |
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79911 | Sisco Laboratories | 25 Gms | 3.9 EUR |
Description: Part B |
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p-Toluenesulphonic Acid Monohydrate extrapure AR, ACS, 99% |
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77948 | Sisco Laboratories | 100 Gms | 2.19 EUR |
Description: Part A |
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EDTA Free Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 99% |
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73816 | Sisco Laboratories | 500 Gms | 7.73 EUR |
Description: Part A |
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L-Lactic Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 88% |
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21206 | Sisco Laboratories | 500 ml | 5.13 EUR |
Description: Part A |
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Sodium Pyruvate (Pyruvic Acid Sodium Salt) extrapure AR, 99% |
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23569 | Sisco Laboratories | 25 Gms | 2.55 EUR |
Description: Part B |
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L-(+)-Tartaric Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.7% |
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60301 | Sisco Laboratories | 500 Gms | 4.5 EUR |
Description: Part B |
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3,5-Dinitrobenzoic Acid extrapure AR, ExiPlus, Multi-Compendial, 99% |
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87464 | Sisco Laboratories | 100 Gms | 6.3 EUR |
Description: Part B |
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L-Ascorbic Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.7% |
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40704 | Sisco Laboratories | 100 Gms | 1.71 EUR |
Description: Part A |
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Propionic Acid extrapure AR, ACS, ExiPlus, Multi-Compendial, C3-99.5% (GC) |
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42263 | Sisco Laboratories | 500 ml | 4.79 EUR |
Description: Part A |
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Tungstosilicic Acid Hydrate extrapure AR, ExiPlus, Multi-Compendial |
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67134 | Sisco Laboratories | 25 Gms | 8.21 EUR |
Description: Part A |
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Trifluoroacetic Acid (TFA) extrapure AR, ExiPlus, Multi-Compendial, 99.5% |
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67869 | Sisco Laboratories | 100 ml | 19.29 EUR |
Description: Part A |
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1,2-Naphthoquinone-4- Sulphonic Acid Sodium Salt extrapure AR, 99% |
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45838 | Sisco Laboratories | 5 Gms | 3.75 EUR |
Description: Part B |
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Silica Diol Bonded extrapure |
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30753 | Sisco Laboratories | 5 Gms | 43.35 EUR |
Description: Part B |
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Acetic Acid Glacial extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.9% |
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93602 | Sisco Laboratories | 500 ml | 1.98 EUR |
Description: Part A |
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Silver Oxide extrapure AR, 99% |
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76610 | Sisco Laboratories | 10 Gms | 24.63 EUR |
Description: Part A |
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Silica Cyano Bonded extrapure |
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35228 | Sisco Laboratories | 5 Gms | 44.03 EUR |
Description: Part B |
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Silver Iodide extrapure AR, 99% |
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24607 | Sisco Laboratories | 10 Gms | 17.11 EUR |
Description: Part A |
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Citric Acid Anhydrous extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
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16842 | Sisco Laboratories | 500 Gms | 2.6 EUR |
Description: Part A |
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Oxalic Acid Dihydrate extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
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72425 | Sisco Laboratories | 500 Gms | 2.94 EUR |
Description: Part A |
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Silica DPP-Pd Catalyst extrapure |
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20356 | Sisco Laboratories | 5 Gms | 283.43 EUR |
Description: Part B |
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Silica TEMPO Catalyst extrapure |
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45570 | Sisco Laboratories | 5 Gms | 138.83 EUR |
Description: Part B |
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Silver Phosphate extrapure AR, 99% |
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86887 | Sisco Laboratories | 5 Gms | 13.68 EUR |
Description: Part A |
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3-Methylphenylboronic Acid extrapure (m-Tolylboronic Acid) extrapure, 97% |
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20622 | Sisco Laboratories | 1 Gms | 6.3 EUR |
Description: Part B |
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Silica Carbonate Bonded extrapure |
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43139 | Sisco Laboratories | 5 Gms | 69.45 EUR |
Description: Part B |
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Silica C18 (17%) Bonded monomeric extrapure |
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78339 | Sisco Laboratories | 5 Gms | 39.45 EUR |
Description: Part B |
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Linoleic Acid (Free Acid) extrapure, 98% |
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98099 | Sisco Laboratories | 5 ml | 22.5 EUR |
Description: Part B |
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Sulphanilic Acid (High Purity) extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
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97979 | Sisco Laboratories | 100 Gms | 3.22 EUR |
Description: Part A |
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Silver Sulphate extrapure AR, ACS, 99% |
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59940 | Sisco Laboratories | 10 Gms | 19.71 EUR |
Description: Part A |
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Nalidixic Acid free acid extrapure, 99% |
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20346 | Sisco Laboratories | 1 Gms | 2.25 EUR |
Description: Part B |
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Oleic Acid extrapure, 98% |
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55081 | Sisco Laboratories | 25 ml | 20.53 EUR |
Description: Part A |
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Mucic Acid extrapure, 99% |
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14189 | Sisco Laboratories | 25 Gms | 5.18 EUR |
Description: Part B |
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Boric Acid extrapure, 99.5% |
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18952 | Sisco Laboratories | 500 Gms | 2.39 EUR |
Description: Part A |
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Kojic acid extrapure, 99% |
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19744 | Sisco Laboratories | 5 Gms | 1.58 EUR |
Description: Part B |
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Osmic Acid extrapure, 99% |
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83577 | Sisco Laboratories | 1 Gms | 51.73 EUR |
Description: Part A |
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Tiglic Acid extrapure, 98% |
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90196 | Sisco Laboratories | 25 Gms | 9.83 EUR |
Description: Part B |
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Quinic Acid extrapure, 98% |
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81076 | Sisco Laboratories | 5 Gms | 8.48 EUR |
Description: Part B |
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Cholic Acid extrapure, 98% |
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54908 | Sisco Laboratories | 25 Gms | 25.5 EUR |
Description: Part B |
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Picric Acid extrapure, 99% |
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94847 | Sisco Laboratories | 100 Gms | 2.53 EUR |
Description: Part A |
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Silver Diethyldithiocarbamate extrapure AR |
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14417 | Sisco Laboratories | 5 Gms | 12.38 EUR |
Description: Part A |
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Behenic Acid extrapure, 99% |
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35591 | Sisco Laboratories | 5 Gms | 30 EUR |
Description: Part B |
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Succinic Acid extrapure, 99% |
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96330 | Sisco Laboratories | 100 Gms | 2.05 EUR |
Description: Part A |
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Cinnamic Acid extrapure, 99% |
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29955 | Sisco Laboratories | 250 Gms | 5.95 EUR |
Description: Part A |
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Trimesic Acid extrapure, 98% |
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57355 | Sisco Laboratories | 5 Gms | 5.55 EUR |
Description: Part B |
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Benzilic Acid extrapure, 99% |
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93528 | Sisco Laboratories | 100 Gms | 9.6 EUR |
Description: Part B |
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Periodic Acid extrapure, 99% |
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26312 | Sisco Laboratories | 25 Gms | 6.5 EUR |
Description: Part A |
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Vanillic Acid extrapure, 99% |
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83080 | Sisco Laboratories | 25 Gms | 8.76 EUR |
Description: Part A |
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Pimelic Acid (Heptanedioic acid) extrapure, 98% |
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90670 | Sisco Laboratories | 25 Gms | 24.68 EUR |
Description: Part B |
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Silica TAAcOH-Functionalized extrapure |
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68360 | Sisco Laboratories | 5 Gms | 72 EUR |
Description: Part B |
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Silica Thiol-Functionalized (STF) extrapure |
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39343 | Sisco Laboratories | 5 Gms | 60.23 EUR |
Description: Part B |
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EDTA Free Acid extrapure, 99% |
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40648 | Sisco Laboratories | 100 Gms | 1.57 EUR |
Description: Part A |
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Arachidic Acid extrapure, 99% |
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89657 | Sisco Laboratories | 1 Gms | 18.75 EUR |
Description: Part B |
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Silica TAAcONa-Functionalized extrapure |
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28466 | Sisco Laboratories | 5 Gms | 72 EUR |
Description: Part B |
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p-Anisic Acid extrapure, 98% |
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90820 | Sisco Laboratories | 100 Gms | 4.43 EUR |
Description: Part B |
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Thorin extrapure AR |
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41862 | Sisco Laboratories | 1 Gms | 18 EUR |
Description: Part B |
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Isobutyric Acid extrapure, 99% |
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39634 | Sisco Laboratories | 500 ml | 7.5 EUR |
Description: Part B |
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Citrazinic Acid extrapure, 97% |
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41694 | Sisco Laboratories | 25 Gms | 16.5 EUR |
Description: Part B |
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Oxalacetic Acid extrapure, 98% |
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62704 | Sisco Laboratories | 1 Gms | 8.33 EUR |
Description: Part B |
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Barbituric Acid extrapure, 99% |
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16411 | Sisco Laboratories | 100 Gms | 5.47 EUR |
Description: Part A |
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Silica TMA Acetate Bonded non-endcapped extrapure |
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92868 | Sisco Laboratories | 5 Gms | 61.8 EUR |
Description: Part B |
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Silica Cysteine-Functionalized extrapure |
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77996 | Sisco Laboratories | 5 Gms | 149.78 EUR |
Description: Part B |
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R-(-)-Mandelic Acid extrapure, 99% |
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58199 | Sisco Laboratories | 25 Gms | 6.9 EUR |
Description: Part B |
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S-(+)-Mandelic Acid extrapure, 99% |
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30727 | Sisco Laboratories | 25 Gms | 6.9 EUR |
Description: Part B |
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D-(-)-Tartaric Acid extrapure, 99% |
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28682 | Sisco Laboratories | 100 Gms | 4.05 EUR |
Description: Part B |
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Methacrylic Acid extrapure, 99% |
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86556 | Sisco Laboratories | 500 ml | 3.69 EUR |
Description: Part A |
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Chlorogenic Acid extrapure, 98% |
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40881 | Sisco Laboratories | 100 Mg | 3.75 EUR |
Description: Part B |
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Deoxycholic Acid extrapure, 99% |
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41143 | Sisco Laboratories | 25 Gms | 27 EUR |
Description: Part B |
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Xanthurenic Acid extrapure, 96% |
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62630 | Sisco Laboratories | 500 Mg | 24 EUR |
Description: Part B |
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Caproic Acid extrapure, C6-99% |
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89028 | Sisco Laboratories | 100 ml | 2.05 EUR |
Description: Part A |
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Homovanillic Acid extrapure, 99% |
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69080 | Sisco Laboratories | 250 Mg | 8.78 EUR |
Description: Part B |
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Pentadecylic Acid extrapure, 98% |
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38215 | Sisco Laboratories | 5 Gms | 18.75 EUR |
Description: Part B |
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DL-6,8-Thioctic Acid extrapure (a-Lipoic Acid), 98% |
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46913 | Sisco Laboratories | 1 Gms | 5.33 EUR |
Description: Part B |
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DL-Tartaric Acid extrapure, 99% |
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63904 | Sisco Laboratories | 500 Gms | 3.98 EUR |
Description: Part B |
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Stearic Acid extrapure, C18-99% |
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73878 | Sisco Laboratories | 5 Gms | 2.25 EUR |
Description: Part B |
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Polyguluronic Acid extrapure, 80% |
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67258 | Sisco Laboratories | 1 Gms | 108.75 EUR |
Description: Part B |
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Phenylboronic Acid extrapure, 95% |
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92890 | Sisco Laboratories | 25 Gms | 14.25 EUR |
Description: Part B |
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Phenoxyacetic Acid extrapure, 99% |
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95444 | Sisco Laboratories | 100 Gms | 3.83 EUR |
Description: Part B |
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Urea extrapure AR, 99.5% |
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69120 | Sisco Laboratories | 500 Gms | 4.24 EUR |
Description: Part A |
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Alar extrapure AR, 99% |
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38211 | Sisco Laboratories | 1 Gms | 3.53 EUR |
Description: Part B |
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D-Glucuronic Acid extrapure, 98% |
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88229 | Sisco Laboratories | 1 Gms | 7.13 EUR |
Description: Part B |
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Chromotropic Acid Disodium Salt Dihydrate extrapure AR, ACS, ExiPlus, Multi-Compendial, 99% |
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72580 | Sisco Laboratories | 10 Gms | 7.73 EUR |
Description: Part B |
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BOC-Pipecolic Acid extrapure, 99% |
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53665 | Sisco Laboratories | 10 Gms | 44.1 EUR |
Description: Part B |
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BOC-Nipecotic Acid extrapure, 99% |
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91418 | Sisco Laboratories | 10 Gms | 47.93 EUR |
Description: Part B |
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Trichloroacetic Acid extrapure, 99% |
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90544 | Sisco Laboratories | 100 Gms | 1.37 EUR |
Description: Part A |
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Isobutylboronic Acid extrapure, 98% |
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34516 | Sisco Laboratories | 1 Gms | 11.48 EUR |
Description: Part B |
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Orthophosphoric Acid extrapure, 85% |
|||
36678 | Sisco Laboratories | 500 ml | 4.11 EUR |
Description: Part A |
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Benzohydroxamic Acid extrapure, 99% |
|||
17851 | Sisco Laboratories | 10 Gms | 33.83 EUR |
Description: Part B |
|||
D-Aspartic Acid extrapure CHR, 99% |
|||
90601 | Sisco Laboratories | 5 Gms | 6 EUR |
Description: Part B |
|||
L-Aspartic Acid extrapure CHR, 99% |
|||
84025 | Sisco Laboratories | 100 Gms | 2.74 EUR |
Description: Part A |
|||
Z-L-Glutamic Acid extrapure, 99% |
|||
67271 | Sisco Laboratories | 5 Gms | 2.7 EUR |
Description: Part B |
|||
Coenzyme A Free Acid extrapure, 90% |
|||
40804 | Sisco Laboratories | 10 Mg | 24.83 EUR |
Description: Part B |
|||
D-Glutamic Acid extrapure CHR, 99% |
|||
61126 | Sisco Laboratories | 1 Gms | 4.73 EUR |
Description: Part B |
|||
Z-L-Aspartic Acid extrapure, 99% |
|||
63143 | Sisco Laboratories | 5 Gms | 3.45 EUR |
Description: Part B |
|||
L-Glutamic Acid extrapure CHR, 99% |
|||
20149 | Sisco Laboratories | 100 Gms | 2.39 EUR |
Description: Part A |
|||
Z-D-Aspartic Acid extrapure, 99% |
|||
32899 | Sisco Laboratories | 1 Gms | 4.8 EUR |
Description: Part B |
|||
Z-D-Glutamic Acid extrapure, 98% |
|||
73971 | Sisco Laboratories | 1 Gms | 5.7 EUR |
Description: Part B |
|||
Isatin extrapure AR, 99% |
|||
90354 | Sisco Laboratories | 25 Gms | 7.05 EUR |
Description: Part A |
|||
Xylene extrapure AR, 99.5% |
|||
90998 | Sisco Laboratories | 500 ml | 3.08 EUR |
Description: Part A |
|||
Biuret extrapure AR, 97% |
|||
17798 | Sisco Laboratories | 25 Gms | 7.87 EUR |
Description: Part A |
|||
Z-Isonipecotic Acid extrapure, 99% |
|||
92186 | Sisco Laboratories | 10 Gms | 30 EUR |
Description: Part B |
|||
a-Ketoglutaric Acid extrapure, 99% |
|||
93929 | Sisco Laboratories | 25 Gms | 4.58 EUR |
Description: Part B |
|||
3-Bromobenzoic Acid extrapure, 99% |
|||
20107 | Sisco Laboratories | 10 Gms | 6.68 EUR |
Description: Part B |
|||
L-Pyroglutamic Acid extrapure, 99% |
|||
77577 | Sisco Laboratories | 25 Gms | 4.73 EUR |
Description: Part B |
|||
6-Aminocaproic Acid extrapure, 99% |
|||
82623 | Sisco Laboratories | 25 Gms | 8.14 EUR |
Description: Part A |
|||
Vanillylmandelic Acid extrapure, 99% |
|||
72241 | Sisco Laboratories | 250 Mg | 9.75 EUR |
Description: Part B |
|||
Isopropylboronic Acid extrapure, 97% |
|||
45273 | Sisco Laboratories | 1 Gms | 22.28 EUR |
Description: Part B |
|||
Z-DL-Aspartic Acid extrapure, 99% |
|||
84275 | Sisco Laboratories | 5 Gms | 5.25 EUR |
Description: Part B |
|||
DL-Aspartic Acid extrapure CHR, 99% |
|||
64848 | Sisco Laboratories | 100 Gms | 3.83 EUR |
Description: Part A |
|||
Propionic Acid extrapure, C3-99% (GC) |
|||
12931 | Sisco Laboratories | 500 ml | 3.28 EUR |
Description: Part A |
|||
Toluene extrapure AR, 99.5% |
|||
85577 | Sisco Laboratories | 500 ml | 2.39 EUR |
Description: Part A |
|||
Kinetin extrapure AR, 99% |
|||
61341 | Sisco Laboratories | 1 Gms | 2.03 EUR |
Description: Part B |
|||
Anisole extrapure AR, 99.5% |
|||
64447 | Sisco Laboratories | 500 ml | 7.58 EUR |
Description: Part B |
|||
Glycine extrapure AR, 99.5% |
|||
66327 | Sisco Laboratories | 500 Gms | 4.45 EUR |
Description: Part A |
|||
Aniline extrapure AR, 99.5% |
|||
22285 | Sisco Laboratories | 500 ml | 3.83 EUR |
Description: Part A |
|||
Benzene extrapure AR, 99.7% |
|||
16410 | Sisco Laboratories | 500 ml | 1.71 EUR |
Description: Part A |
|||
SPADNS extrapure AR, ACS |
|||
47886 | Sisco Laboratories | 1 Gms | 2.74 EUR |
Description: Part A |
|||
Methyl Red extrapure AR |
|||
49449 | Sisco Laboratories | 25 Gms | 1.71 EUR |
Description: Part A |
|||
m-Chlorobenzoic Acid extrapure, 99% |
|||
34366 | Sisco Laboratories | 25 Gms | 6.08 EUR |
Description: Part B |
|||
2-Ethylhexanoic Acid extrapure, 99% |
|||
78214 | Sisco Laboratories | 250 ml | 1.88 EUR |
Description: Part B |
|||
BOC-L-Glutamic acid extrapure, 99% |
|||
69376 | Sisco Laboratories | 10 Gms | 8.03 EUR |
Description: Part B |
|||
BOC-L-Aspartic acid extrapure, 99% |
|||
30394 | Sisco Laboratories | 5 Gms | 12 EUR |
Description: Part B |
|||
BOC-D-Glutamic Acid extrapure, 99% |
|||
92584 | Sisco Laboratories | 10 Gms | 40.65 EUR |
Description: Part B |
|||
BOC-D-Aspartic Acid extrapure, 99% |
|||
38230 | Sisco Laboratories | 10 Gms | 29.33 EUR |
Description: Part B |
|||
Phosphorous Acid Crystals (Phosphonic Acid) extrapure, 98% |
|||
78861 | Sisco Laboratories | 500 Gms | 5.75 EUR |
Description: Part A |
|||
Sucrose extrapure AR, ACS |
|||
90701 | Sisco Laboratories | 500 Gms | 3.01 EUR |
Description: Part A |
|||
Anthrone extrapure AR, 98% |
|||
96476 | Sisco Laboratories | 10 Gms | 3.98 EUR |
Description: Part B |
|||
Methanol extrapure AR, 99.8% |
|||
65524 | Sisco Laboratories | 500 ml | 1.85 EUR |
Description: Part A |
|||
Hexamine extrapure AR, 99.5% |
|||
92391 | Sisco Laboratories | 500 Gms | 3.22 EUR |
Description: Part A |
|||
Vanillin extrapure AR, 99% |
|||
20563 | Sisco Laboratories | 25 Gms | 2.33 EUR |
Description: Part A |
|||
Carbinol extrapure AR, 99.8% |
|||
82911 | Sisco Laboratories | 500 ml | 1.85 EUR |
Description: Part A |
|||
n-Butyric Acid extrapure, C4-99% (GC) |
|||
81503 | Sisco Laboratories | 500 ml | 3.63 EUR |
Description: Part A |
|||
BOC-Isonipecotic Acid extrapure, 99% |
|||
55665 | Sisco Laboratories | 10 Gms | 47.93 EUR |
Description: Part B |
|||
3,5-Dinitrobenzoic Acid extrapure, 98% |
|||
58586 | Sisco Laboratories | 100 Gms | 4.2 EUR |
Description: Part B |
|||
3,5-Diaminobenzoic Acid extrapure, 98% |
|||
17758 | Sisco Laboratories | 100 Gms | 6.9 EUR |
Description: Part B |
|||
Dithizone extrapure AR, 85% |
|||
89257 | Sisco Laboratories | 5 Gms | 6.75 EUR |
Description: Part B |
|||
Ninhydrin extrapure AR, 99% |
|||
55058 | Sisco Laboratories | 10 Gms | 4.79 EUR |
Description: Part A |
|||
Isooctane extrapure AR, 99.5% |
|||
57075 | Sisco Laboratories | 500 ml | 7.39 EUR |
Description: Part A |
|||
Bilirubin extrapure AR, 99% |
|||
64839 | Sisco Laboratories | 100 Mg | 6.53 EUR |
Description: Part B |
|||
Imidazole extrapure AR, 99% |
|||
32822 | Sisco Laboratories | 100 Gms | 4.45 EUR |
Description: Part A |
|||
Formamide extrapure AR, 99.5% |
|||
71714 | Sisco Laboratories | 500 ml | 3.97 EUR |
Description: Part A |
|||
FMOC-L-Glutamic Acid extrapure, 99% |
|||
86254 | Sisco Laboratories | 10 Gms | 37.43 EUR |
Description: Part B |
|||
FMOC-L-Aspartic Acid extrapure, 99% |
|||
30074 | Sisco Laboratories | 1 Gms | 51.38 EUR |
Description: Part B |
|||
L-a-Amino Adipic Acid extrapure, 98% |
|||
35418 | Sisco Laboratories | 250 Mg | 20.18 EUR |
Description: Part B |
|||
D-a-Amino Adipic Acid extrapure, 98% |
|||
62360 | Sisco Laboratories | 250 Mg | 23.1 EUR |
Description: Part B |
|||
2,4-Dichlorobenzoic Acid extrapure, 98% |
|||
62122 | Sisco Laboratories | 100 Gms | 2.63 EUR |
Description: Part B |
|||
3-Pyridineboronic Acid extrapure, 98% |
|||
73512 | Sisco Laboratories | 1 Gms | 15.3 EUR |
Description: Part B |
|||
5-Sulphosalicylic Acid extrapure, 99% |
|||
44445 | Sisco Laboratories | 250 Gms | 3.83 EUR |
Description: Part A |
|||
trans-1,2-Diaminocyclohexane-N,N,N,N Tetraacetic Acid Monohydrate extrapure AR (CDTA), 99% |
|||
16368 | Sisco Laboratories | 10 Gms | 10.58 EUR |
Description: Part B |
|||
Isobutanol extrapure AR, 99.5% |
|||
98646 | Sisco Laboratories | 500 ml | 3.08 EUR |
Description: Part A |
|||
Chloroform extrapure AR, 99.5% |
|||
84155 | Sisco Laboratories | 500 ml | 2.33 EUR |
Description: Part A |
Furthermore, we offer deep-learning fashions that predict the actions of xCas9 and SpCas9-NG on the goal sequences. The ensuing deeper understanding of the actions of xCas9, SpCas9-NG and SpCas9 in human cells ought to facilitate their use.
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